%0 Journal Article %T Evaluation of Cisplatin Efficacy on HepG2 and E. coli Cells under Acidic Conditions %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %A Babaei, Faezeh %A Ebrahimi Shahmabadi, Hasan %A Rajabi, Mohammad Reza %A Haddad Kashani, Hamed %A Izadpanah, Fatemeh %D 2019 %\ 03/01/2019 %V 20 %N 3 %P 723-726 %! Evaluation of Cisplatin Efficacy on HepG2 and E. coli Cells under Acidic Conditions %K Acidic cisplatin %K HepG2 %K MIC assay %K MTT assay %K Standard cisplatin %R 10.31557/APJCP.2019.20.3.723 %X Background: Cisplatin (Cispt) is a common anticancer drug for the treatment of several malignancies, includinghepatocarcinoma. However, this drug suffers from instability in aqueous solutions. The study aimed to evaluate cisplatinefficacy on HepG2 and E. coli cells under an acidic condition. Methods: Acidic Cispt was prepared via incubation inacidic condition (pH=2) for a month duration. The chemical structure of the acidic Cispt was evaluated by using FourierTransform Infrared Spectroscopy (FTIR) method. The cytotoxicity of the standard and acidic Cispt were then determinedby 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and minimum inhibitory concentration (MIC)assays on HepG2 and E. coli cells, respectively. Results: After preparing of acidic Cispt, its chemical structure wasdetermined by FTIR method. In addition, cytotoxicity effects of Cispt in the standard and acidic forms were calculated58 ± 2.9 and 65 ± 3.25 μM, respectively. MIC results also confirmed the results of MTT assay. MIC results for thestandard and acidic Cispt were estimated 9.5 ± 0.47 and 9.8 ± 0.49 μM, respectively. Conclusion: Preparing Cispt inacidic condition not only did not degrade the drug, but also kept the potency of the drug approximately equal to parentdrug. Regarding the instability issues of Cispt, keeping Cispt in acidic condition could be a promising approach topreserve its efficacy. %U https://journal.waocp.org/article_81620_e525d17e475a1f8d0a0a9686e674cc4f.pdf