%0 Journal Article %T Protease Cargo in Circulating Exosomes of Breast Cancer and Ovarian Cancer Patients %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %A Tamkovich, Svetlana N %A Yunusova, Natalia V %A Tugutova, Elena %A Somov, Anton K %A Proskura, Ksenia V %A Kolomiets, Larisa A %A Stakheyeva, Marina N %A Grigor’eva, Alina E %A Laktionov, Pavel P %A Kondakova, Irina V %D 2019 %\ 01/01/2019 %V 20 %N 1 %P 255-262 %! Protease Cargo in Circulating Exosomes of Breast Cancer and Ovarian Cancer Patients %K exosomes %K ADAM-10 %K 20S proteasome %K breast cancer %K Ovarian Cancer %R 10.31557/APJCP.2019.20.1.255 %X Background: As is known, exosomes play an important role in promoting progression of cancers by increasingits invasive potential. The aim of this study was to evaluate the levels of tetraspanine-associated (ADAM-10) andtetraspanine-nonassociated proteases (20S proteasomes) in exosomes from culture medium, plasma exosomes ofpatients with breast tumors and plasma and ascites of ovarian tumor patients. Methods: MCF-7 and SVO-3 culturemediums and blood samples from healthy females (n = 30, HFs), patients with diffuse dyshormonal dysplasia of thebreast (n=28, BBTPs), breast cancer patients (n=32, BCPs), borderline ovarian tumor patients (n=20, BOTPs) andblood and ascites samples ovarian cancer patients (n=35, OCPs) were included in the study. Exosomes from plasma,ascites and culture mediums were isolated and characterized in according to Extracellular Vesicles Society. Theexpression levels of 20S proteasome and ADAM-10 in exosomes were determined using flow cytometry and westernblot analysis, correspondingly. Results: The subpopulation composition of the exosomes from MCF-7 culture mediumand from blood plasma of HFs and breast diseases patients is similar, however CD9/CD24 subpopulation significantlyincreased at cell supernatant. The similar results was obtained for exosomes from SVO-3 medium and blood plasmaand ascites of ovary tumor patients, but CD9/CD24 subpopulation significantly decreased at cells and illness samples,however CD63/CD24 exosomes increased significantly from cell supernatant. 20S proteasome level is significantlyincreased in exosomes from MCF-7 and SVO-3 culture medium, breast tumor patients and OCPs plasma in comparisonto HUVEC culture medium and HFs plasma samples. At CD9-positive exosomes from BCPs plasma and MCF-7 wasreveal a high expression of ADAM-10 and low expression is from BBDPs plasma and ovarian tumor patients plasma/ascites samples. Exosomes from ascites OCP had high expression of ADAM-10 in the CD24-positive subpopulation.Conclusion: Breast and ovarian cancer development is connected with functioning of immune proteasome forms inplasma and ascites exosomes, while increased ADAM10 expression at CD9-positive exosome was associated withbreast cancer and at CD24-positive subpopulation – with ovarian cancer. Obtained data confirm role of exosomalproteases in tumor progression. %U https://journal.waocp.org/article_81713_5de6d9c24facd37fb33a65b8943b371d.pdf