%0 Journal Article %T Plasma Peptidome Pattern of Breast Cancer Using Magnetic Beads-Based Plasma Fractionation and MALDI-TOF MS: A Case Control Study in Egypt %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %A Zaki, Ahmad %A Ramadan, Ragaa Abdelkader %A Moez, Pacint %A Ghareeb, Hala %A Elkarmouty, Ahmad %D 2019 %\ 01/01/2019 %V 20 %N 1 %P 175-184 %! Plasma Peptidome Pattern of Breast Cancer Using Magnetic Beads-Based Plasma Fractionation and MALDI-TOF MS: A Case Control Study in Egypt %K breast cancer %K Matrix assisted laser desorption ionization/ time of flight mass spectrometry %K magnetic beads %K ClinProTools %R 10.31557/APJCP.2019.20.1.175 %X Objective: The present study aimed to determine peptidome patterns in breast cancer (BC). Methods: We analyzedthe plasma proteomic profiling of 80 BC patients and 50 healthy controls, using hydrophobic interaction chromatographymagnetic beads (MB-HIC8) separation followed by Matrix assisted laser desorption ionization/ time of flight massspectrometry (MALDI-TOF MS). Results: ClinProTools software identified 92 peaks that differed among the analyzedgroups, 33 peaks were significantly different (P < 0.05). Of those, 22 peaks were up-regulated while 11 peaks weredown-regulated in BC patients compared with the healthy controls. Three peptide ion signatures (m/z 1,570.31, 1,897.4and 2,568.17) were provided by the Quick Classifier model to discriminate BC patients from healthy control subjectswith 96.4% accuracy. External validation was performed by an independent group and this achieved a sensitivity of100% and a specificity of 76.9%. Conclusion: MALDI-TOF MS has good analytical performance in distinguishingBC patients from healthy controls. %U https://journal.waocp.org/article_81799_0a45dba6ceef92586c4393c6e66fb247.pdf