%0 Journal Article %T Targeting the BRAF Signaling Pathway in CD133pos Cancer Stem Cells of Anaplastic Thyroid Carcinoma %J Asian Pacific Journal of Cancer Prevention %I West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter. %Z 1513-7368 %A Bozorg-Ghalati, Farzaneh %A Hedayati, Mehdi %A Dianatpour, Mehdi %A Mosaffa, Narimann %A Azizi, Fereidoun %D 2019 %\ 05/01/2019 %V 20 %N 5 %P 1353-1360 %! Targeting the BRAF Signaling Pathway in CD133pos Cancer Stem Cells of Anaplastic Thyroid Carcinoma %K Anaplastic thyroid carcinoma %K Positive CD133 cancer stem cells %K BRAF inhibition %K Sodium-iodide symporter %R 10.31557/APJCP.2019.20.5.1353 %X Background: Cancer stem cells (CSCs) with a self-renewal ability in tumor cells population, execute a pivotalfunction in tumorigenesis, retrogression, and metastasis of malignant cancers such as anaplastic thyroid carcinoma(ATC). Materials and Methods: In this study, we isolated CSCs subpopulation with CD133 surface marker fromthree ATC cell lines by magnetic cell sorting assay. After confirming the segregation by the flow cytometry method,BRAF and sodium-iodide symporter (NIS) genes were investigated in them before and after incubation with BRAFinhibitor. Also, we evaluated the NIS protein expression and localization. Results: Established upon q-RT PCR data,when compared to human normal thyrocytes, the BRAFV600E gene was over-expressed in CD133pos cells (>1705.99 ±55.55 fold, Mean ± SEM, n=3, P- value<0.05), whilst the expression of NIS gene was very restricted (< 0.0008 ± 5.43fold, Mean ± SEM, n=3, P- value<0.05) in them. Also, our results showed that BRAF inhibition affected NIS proteinexpression and localization. Conclusions: Current study showed that the differentiate genes/proteins expression canbe induced in the CSCs via focus on signal transduction pathways and targeting their molecules, that are involved inexpression of these genes/proteins. Therefore, attention to targeting CSCs along with routine thyroid cancer therapy,can help to ATC treatment. %U https://journal.waocp.org/article_86820_058f5512033a587709b144839c181686.pdf