ORIGINAL_ARTICLE
Association of MTHFR 677C>T Polymorphism with Susceptibility to Ovarian and Cervical Cancers: A Systematic Review and Meta-Analysis
Background: Previous studies have evaluated the impact of MTHFR 677C>T polymorphism on susceptibility toovarian and cervical cancers in women, but the conclusions are still controversial. To get a more precise evaluation ofthe association between MTHFR 677C>T polymorphism and risk of ovarian and cervical cancers, we performeda meta-analysis of the association of all eligible studies. Methods: A comprehensive search performed in PubMed,Google Scholar, CNKI, and Web of Science databases to identify the relevant studies up to October 15, 2018. Thestrength of the association was estimated by odds ratios (OR) with 95% confidence interval (CI). Results: A total of 27case-control studies including eleven studies with 4990 cases 7730 controls on ovarian cancer and 16 studies with 4990cases and 7730 controls on cervical cancer were selected. Pooled data revealed that the MTHFR 677C>T polymorphismnot significantly associated with an increased risk of ovarian and cervical cancers under all five genetic models.However, stratified analysis by ethnicity showed that the MTHFR 677C>T polymorphism was significantly associatedwith risk of ovarian cancer in Asians. No publication bias was found in the current meta-analysis. Conclusions: Theresults of this meta-analysis proposes that the MTHFR 677C>T polymorphism may not play a role in development ofovarian and cervical cancers in overall population. Further well-designed studies are necessary to clarify the preciserole of the MTHFR 677C>T polymorphism on ovarian and cervical cancers risk.
https://journal.waocp.org/article_88718_c600934455eea15d9188e6a17b4bf049.pdf
2019-09-01
2569
2577
10.31557/APJCP.2019.20.9.2569
Ovarian Cancer
Cervical cancer
MTHFR gene
Polymorphism
Meta-analysis
Mojgan
Karimi-Zarchi
mk.zarchi55@gmail.com
1
Department of Gynecology and Obstetrics, Iran University of Medical Sciences, Tehran, Iran.
AUTHOR
Mansour
Moghimi
shilrzfbxgey81@yahoo.com
2
Department of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
LEAD_AUTHOR
Hajar
Abbasi
3
Department of Gynecology and Obstetrics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Amaneh
Hadadan
mor.fattahi76@gmail.com
4
Department of Gynecology and Obstetrics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Erfaneh
Salimi
5
Department of Gynecology and Obstetrics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Majid
Morovati-Sharifabad
6
Department of Basic Science, Faculty of Veterinary Medicine, Ardakan University, Ardakan, Iran.
AUTHOR
Mohammad Javad
Akbarian-Bafghi
7
Department of Healthcare Management, Bam University of Medical Sciences, Bam, Iran.
AUTHOR
Masoud
Zare-Shehneh
8
Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
AUTHOR
Alireza
Mosavi-Jarrahi
journal@waocp.org
9
Department of Social Medicine, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Hossein
Neamatzadeh
neamatzadehh@gmail.com
10
Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
AUTHOR
ORIGINAL_ARTICLE
Colposcopic and Histological Outcome of Atypical Squamous Cells of Undetermined Significance and Atypical Squamous Cell of Undetermined Significance Cannot Exclude High-Grade in Women Screened for Cervical Cancer
Objectives: The objectives of the study are to assess the prevalence of colposcopic and histological abnormalities in patients diagnosed with ASCUS and ASC-H and to compare the prevalence of CIN in each group. Methods: Population-based cross-sectional retrospective study was conducted in one of tertiary hospitals in UAE. All cervical smears reported as ASCUS or ASC-H in 2015 were included in this study. The local guideline in 2015 was to refer all cases of ASC for colposcopy assessment. Results: Overall 7,418 cervical smears were processed at our laboratory service, 5.6% (n=413) were reported as ASC. 95% of them (n=394) were ASCUS and 5% (n=19) were ASC-H. The overall prevalence of high grade CIN in patients with ASC-H is 26% compared with 0.8% for patients with ASCUS regardless the age. The relative risk of patients with ASC-H is 8 folds higher than patients with ASCUS to have low grade CIN but 29 fold higher risk of having High grade CIN and the P value =0.0001.Conclusion: ASC-H cytology confers a substantially higher risk for high grade CIN than ASCUS regardless of age. HPV test is an important triage test in patients with ASCUS to predict cellular changes and CIN.
https://journal.waocp.org/article_88744_c44b024d13ac38d82d906633c7ba1032.pdf
2019-09-01
2579
2582
10.31557/APJCP.2019.20.9.2579
cervical smear
Pap smear
ASCUS
Osman
Ortashi
osmanortashi@hotmail.com
1
Sidra Medical and Research Center, Qatar, Specialist Physician, Womens Health Institute, Al Ain Hopital, United Arab Emirates.
LEAD_AUTHOR
Dana
Abdalla
dhabdulla@seha.ae
2
Sidra Medical and Research Center, Qatar, Specialist Physician, Womens Health Institute, Al Ain Hopital, United Arab Emirates.
AUTHOR
ORIGINAL_ARTICLE
Detecting Important Risk Factors of Survival Time of Lung Cancer Patients Using Censored Quintile Regression
Objective: Lung cancer is the most frequently diagnosed cancer and the most leading cause of death due to cancerworldwide. This study aimed to use censored quintile regression model to estimate the effect of potential risk factorson survival of lung cancer patients. Methods: In this study we used a dataset of a retrospective cohort study conductedin West Azerbaijan (during 2007 and 2014). Demographic variables included age, and gender and biological variablesincluded Eastern Cooperative Oncology Group, smoking, tumor type, stage, metastasis, and treatment were investigatedas risk factors of survival of lung cancer patients by using censored quintile regression. Result: The mean (± standarddeviation) age of the 347 lung cancer patients was 63.48 (± 13.55) years. The survival time ranges from 11 to 91. Atotal of 240 (67.6%) experienced death by the end of the study. The impact of Eastern Cooperative Oncology Group(IV), smoking and treatment were significant for most of quintiles (p<0.05). Stage of cancer and metastasis are the otheraffective risk factors on the survival of lung cancer patients(p<0.05). It was shown that Eastern Cooperative Oncology,smoking habit and treatment were significantly associated with a shorter time-to-death progression. Conclusion: Ascensored quintile regression could consider time-varying effects and the interpretation of its regression coefficients areeasy it could be an adequate choice for analyzing survival data.
https://journal.waocp.org/article_88738_683057514350f16ddc5e1ad68066a619.pdf
2019-09-01
2583
2588
10.31557/APJCP.2019.20.9.2583
Censored Quintile Regression
Lung cancer
survival
Cohort studies
Payam
Amini
payam.amini87@gmail.com
1
Department of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
AUTHOR
Malek
Abazari
abazari.malek@gmail.com
2
Department of Public Health, School of Public Health, Ardabil University of Medical Sciences, Ardabil, Iran.
AUTHOR
Behnaz
Alafchi
be.alafchi@edu.umsha.ac.ir
3
Department of Biostatistics, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Perceptions Regarding Tobacco Cessation Counselling among Dental Students and Graduates: A Cross-Sectional Study
Background: Tobacco usage is a global concern and it is essential to curb its usage and increase awarenessamong patients. Dental professionals’ contribution in tobacco cessation will definitely make a stark difference. Thusit is important to assess dental students’ and graduates’ knowledge, attitude and practice towards Tobacco cessationcounselling (TCC). Materials and Methods: The descriptive, cross-sectional survey was carried out among 286students of a dental college in South India. A well-structured, pretested, self-administered questionnaire consistingof 17 close-ended questions was employed to assess knowledge, attitude and practice towards TCC. Results: Almostall study participants agreed that tobacco cessation counselling is under the scope of dental practice and admitted thatit is the responsibility of dental professionals to educate patients for the same. Majority of graduates responded thatthey have done tobacco cessation counselling whereas less than two-third of undergraduates have done the same anddifference between two groups was statistically significant (p<0.001). A significantly higher number of graduates (79%)were aware of 5A’s and 5R’s protocol for TCC as compared to undergraduates (50%). But less than one-fifth of studypopulation have actually applied this protocol for TCC in clinical practice. Conclusion: There is a need to modifythe dental curriculum and motivate young graduates and students about TCC. This will develop a more professionalcompetence and helps to encourage the dental professionals in developing a preventive mind set about tobacco use.Thus, it will help in enhancing the long-term rates of quitting tobacco among patients, which will prove beneficial incontrolling tobacco related diseases in near future.
https://journal.waocp.org/article_88739_d06dba0bfa8859912655bb9e9c4c973f.pdf
2019-09-01
2589
2593
10.31557/APJCP.2019.20.9.2589
Tobacco cessation
counselling
dental professionals
role model
Attitude
Priyanka
Kachwaha
prishagun@gmail.com
1
Manipal College of Dental Sciences, Manipal, Manipal Academy of Higher Education, Karnataka, India.
AUTHOR
Deepak Kumar
Singhal
dk.singhal1981@gmail.com
2
Department of Public Health Dentistry, Manipal College of Dental Sciences, Manipal, Manipal Academy of Higher Education, Karnataka, India.
LEAD_AUTHOR
Nishtha
Singh
nishthasingh11@gmail.com
3
Manipal College of Dental Sciences, Manipal, Manipal Academy of Higher Education, Karnataka, India.
AUTHOR
ORIGINAL_ARTICLE
Association of ACE I/D and AGTR1 A1166C Gene Polymorphisms and Risk of Uterine Leiomyoma: A Case-Control Study
Objective: Uterine leiomyoma (UL) can be considered as the most common benign gynecological tumors of the smooth muscle cells in the myometrium. They are likely to be associated with infertility and recurrent abortion as well as obstructed labor and post-partum hemorrhage. Moreover, altered vascular-related genes can be linked to developing leiomyoma. Polymorphisms of the angiotensin-converting enzyme (ACE) gene are associated with some vascular diseases. The present study was carried out to investigate the association of ACE I/D and AGTR1 A1166C gene polymorphisms and the risk of uterine leiomyoma in a sample of Iranian population. Methods: The study was carried out on a total of 413 women divided into 202 patients with diagnosed uterine leiomyomas and a control group of 211. Genotyping was performed using the PCR or PCR-RFLP methods. Results: The ID and DD genotypes of ACE I/D polymorphism were associated with 2 and 2.9 fold higher risk of UL compared to II genotype (OR, 2 [95% CI, 1.3 to 3.2]; P = 0.004 and OR, 2.9 [95% CI, 1.6 to 5]; P = 0.0002). The frequencies of ACE D alleles were 53.7% in women with UL and 40.3% in controls, which were observed to be statistically different (P < 0.0001). The alleles and genotypes of AGTR1 A1166C polymorphism were not different between UL and control women (P=0.9). Conclusion: The ACE ID and DD genotypes were associated with a higher risk of UL. No relationship was found between AGTR1 A1166C polymorphism and UL.
https://journal.waocp.org/article_88753_a7e52df2a7c4a81f751f170fd324e6d7.pdf
2019-09-01
2595
2599
10.31557/APJCP.2019.20.9.2595
ACE
AGTR1
Polymorphism
Uterine leiomyoma
Farshid
Keshavarzi
keshavaezifarshid@gmail.com
1
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
AUTHOR
Batool
Teimoori
teimooribatool@gmail.com
2
Department of Obstetrics and Gynecology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
AUTHOR
Farahnaz
Farzaneh
farahnaz1826@yahoo.com
3
Department of Obstetrics and Gynecology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
AUTHOR
Mojgan
Mokhtari
mmokhtari1345@hotmail.com
4
Department of Obstetrics and Gynecology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
AUTHOR
Darya
Najafi
daryanajafi@gmail.com
5
Medical College, Iran University of Medical Sciences, Tehran, Iran.
AUTHOR
Saeedeh
Salimi
sasalimi@yahoo.com
6
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Microsatellite Instability in Endometrial Carcinoma by Immunohistochemistry, Association with Clinical and Histopathologic Parameters
Objective: We aimed to investigate the frequency of microsatellite instability (MSI) in endometrial carcinoma in ourpopulation and its association with clinico-pathologic features. Methods: A total of 126 cases of primary endometrialcarcinoma were included in the study that underwent surgical resections. All slides of these cases were reviewed andrepresentative paraffin fixed tissue blocks were selected for MLH1, MSH2, MSH6 and PMS2 IHC staining. IHCexpression was categorized into five groups: no loss of expression; loss of expression of all four antibodies; combinedloss of MLH1/PMS2; combined loss of MSH2/MSH6; and isolated loss of MLH1. Pathological records of all caseswere retrieved from patient files. Result: Abnormal expression of MSI was noted in 56 cases (44.4%) among which16 cases showed loss of nuclear expression of all markers, 34 cases showed loss of MLH1/PMS2 expression, 4 casesshowed loss of MSH2/MSH6 while only 2 cases revealed isolated loss of MLH. Personal and family history suggestiveof inherited cancer susceptibility was revealed in 11 cases most of which were associated with MSH2/MSH6 loss.Significant association of MSI expression was found with tumor stage and personal/family history of endometrial/colon cancer. Conclusion: A high frequency of endometrioid cancers in our study showed abnormal expression ofMSI markers, most of which depicted MLH1/PMS2 loss and were not associated with inherited cancer susceptibility.On the other hand, a minority of cases showed loss of all MSI markers or MSH2/MSH6 loss and were significantlyassociated with family/personal history of cancer. Therefore, we suggest that epigenetic changes in MLH1 locus maybe a predominant pathway of tumorigenesis in our population rather than inherited mutation of MSI genes; howevermore large scale studies with genetic testing are required to validate this observation.
https://journal.waocp.org/article_88727_e5c2ad67dd0744316580da637073bc66.pdf
2019-09-01
2601
2606
10.31557/APJCP.2019.20.9.2601
Microsatellite Instability (MSI)
endometrial carcinoma
Lynch syndrome
hereditary nonpolyposis colorectal cancer (HNPCC) syndrome
Atif Ali
Hashmi
atif.hashmi@lnh.edu.pk
1
Department of Histopathology, Liaquat National Hospital and Medical College, Karachi, Pakistan.
LEAD_AUTHOR
Ghazala
Mudassir
ghazalamudassir@yahoo.com
2
Department of Pathology, Shifa College of Medicine, Islamabad, Pakistan.
AUTHOR
Rozina Nooreen
Hashmi
dr.rozina123@gmail.com
3
Department of Physiology, CMH Institute of Medical Sciences, Multan, Pakistan.
AUTHOR
Muhammad
Irfan
irfan.zafar@lnh.edu.pk
4
Department of Statistics, Liaquat National Hospital and Medical College, Karachi, Pakistan,
AUTHOR
Huda
Asif
huda.ha16@gmail.com
5
Medical Student, CMH Institute of Medical Sciences, Multan, Pakistan.
AUTHOR
Erum Yousuf
Khan
erum.yousuf@lnh.edu.pk
6
Department of Histopathology, Liaquat National Hospital and Medical College, Karachi, Pakistan.
AUTHOR
Syed Muhammad
Abu Bakar
syed.abubakar@lnh.edu.pk
7
Department of Histopathology, Liaquat National Hospital and Medical College, Karachi, Pakistan.
AUTHOR
Naveen
Faridi
dr.naveen.faridi@lnh.edu.pk
8
Department of Histopathology, Liaquat National Hospital and Medical College, Karachi, Pakistan.
AUTHOR
ORIGINAL_ARTICLE
Make Intelligent of Gastric Cancer Diagnosis Error in Qazvin’s Medical Centers: Using Data Mining Method
Objective: Gastric cancer is one of the most common types of cancers, which will result in irreparable harm in the case of misdiagnosis or late diagnosis. The purpose of this study is to investigate the capability of data mining techniques and disease risk factor characteristics to predict and diagnose the gastric cancer. Methods: In this retrospective descriptive-analytic study, we selected 405 samples from two groups of patient and healthy participants. A total of 11 characteristics and risk factors were examined. we used four Machine learning methods, Include support vector machine (SVM), decision tree (DT), naive Bayesian model, and k nearest neighborhood (KNN) to classify the patients with gastric cancer. The evaluation criteria to investigate the model on the database of patients with gastric cancer included Recall, Precision, F-score, and Accuracy. Data was analyzed using MATLAB® software, version 3.2 (Mathworks Inc., Natick, MA, USA). Results: Based on the results achieved from the evaluation of four methods, the accuracy rates of SVM, DT, naive Bayesian model, and KNN algorithms were 90.08, 87.89, 87.60, and 87.60 percent, respectively. The findings showed that the highest level of F-Score was related to the SVM (91.99); whereas, the lowest rate was associated with the KNN algorithm (87.17). Conclusion: According to the findings, the SVM algorithm showed the best results in classification of Test samples. So, this intelligent system can be used as a physician assistant in medical education hospitals, where the diagnosis processes are performed by medical students.
https://journal.waocp.org/article_88754_f5d63b936a7bd2c1cfa465fe22638371.pdf
2019-09-01
2607
2610
10.31557/APJCP.2019.20.9.2607
Gastric cancer
risk factors
Early Diagnosis
Data mining
Artificial intelligence
Asghar
Mortezagholi
a.mortezagholi@gmail.com
1
Department of Computer Engineering, Islamic Azad University of Qazvin (QIAU), Qazvin, Iran.
AUTHOR
Omid
Khosravizadeh
omid.khosravizadeh@gmail.com
2
Social Determinants of Health Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
AUTHOR
Mohammad Bagher
Menhaj
r.kalhor@yahoo.com
3
Department of Electrical Engineering, Amirkabir University of Technology, Tehran, Iran.
AUTHOR
Younes
Shafigh
r.kalhor@qums.ac.ir
4
General Surgeon, Assistant Professor. School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
AUTHOR
Rohollah
Kalhor
r.kalhor@gmail.com
5
Social Determinants of Health Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Modified Liquid-Based Cytology Technique for Immunocytochemistry in Effusion Specimen
Objective: Immunocytochemistry (ICC) of serous effusion is an important tool for the diagnosis of benign andmalignant cells. Our aim was to develop a modified liquid-based cytological technique for ICC (i.e., a modified LBC).Methods: Serous effusions of 110 cases were collected for cytological examination: 50 were negative for malignancyalbeit benign mesothelium was found, and 60 were confirmed metastatic adenocarcinoma according to the modified LBCpreparation. The latter were stained for EMA, Ber-EP4, Calretinin, and p63 then interpreted by both a cytotechnologistand a pathologist. A comparative analysis of the diagnostic results was conducted. Results: The results of the metastaticadenocarcinoma were 100% (60/60) positive for EMA and 91.7% (55/60) positive for Ber-Ep4 but negative for calretininand p63. Cases negative for malignancy were 100% (50/50) positive for calretinin but negative for carcinoma markers.The difference between ‘positive for metastatic adenocarcinoma’ and ‘negative for malignancy’ in ICC was statisticallysignificant (p < 0.001). Conclusion: The current study demonstrated that a panel marker, comprising EMA, Ber-EP4,and calretinin can be used for differentiating between cases of metastatic adenocarcinoma and benign mesothelium.The serous effusion specimen collected by the modified LBC technique is an effective preparation method for ICC.
https://journal.waocp.org/article_88735_95aeaae0c09a1b2bf8f010759393b474.pdf
2019-09-01
2611
2617
10.31557/APJCP.2019.20.9.2611
Modified liquid-based cytology
Immunocytochemistry
Cytology
Effusion
Natcha
Patarapadungkit
nuapat@kku.ac.th
1
Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
LEAD_AUTHOR
Porntip
Jangsiriwitayakorn
prangmeme@gmail.com
2
Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
AUTHOR
Surachat
Chaiwiriyakul
cusurac@kku.ac.th
3
Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
AUTHOR
Phannatorn
Sirivech
phannatornso@kku.ac.th
4
Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
AUTHOR
Ratchaneekorn
Thongbor
ratcth@kku.ac.th
5
Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
AUTHOR
Em-Orn
Phanomsri
emornph@yahoo.com
6
Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
AUTHOR
Luxkana
Nititarakul
niticyto@gmail.com
7
Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
AUTHOR
ORIGINAL_ARTICLE
The Effect of Combination of Steroid and L-Asparaginase on Hyperglycemia in Children with Acute Lymphoblastic Leukemia (ALL)
Background: Hyperglycaemia is a common side effect of steroid and L-asparaginase combinations, occurring mostoften during acute lymphoblastic leukemia (ALL) induction phase. To date in Indonesia, it has not been obtained dataon the incidence of hyperglycemia in children with ALL in the induction phase and how the role of combinations ofL-asparaginase and different type of steroid used. The purpose of this study is to determine the incidence of hyperglycemiain children ALL induction phase, knowing the difference between prednisone and dexamethasone (in combination withL-asparaginase) in causing hyperglycemia in children with ALL and determine the relationship of other factors relatedto hyperglycaemia. Methods: This was a prospective analytic study with a pre- and post-test design, conducted inthree hospitals (Cipto Mangunkusumo Hospital, Dharmais Cancer Hospital, and Gatot Soebroto Hospital). Patient’sblood glucose levels were checked at the 3rd (pretest), 4th, 5th and 6th week of protocol (post-test). Result: Of the57 patients, 5.26% had hyperglycemia. The patients’ age ranged from 1.4 years old to 15.8 years old (6.7 years old).There was no relationship between age, CNS infiltration, leukocytosis, Down syndrome, nutritional status, familyhistory of diabetes, infections and ALL stratification with hyperglycemia (p>0.05). Dexamethasone has more chanceof obtaining higher mean rate of change in blood glucose levels compared to prednisone. (p < 0.05; RR 10.68; CI 95%1.52-74.73). Conclusion: The incidence of hyperglycemia in this study is 5.26%. Dexamethasone, in combinationwith L-asparaginase, despite having no difference in causing hyperglycemia, has an increased risk of changing bloodglucose levels compared to prednisone.
https://journal.waocp.org/article_88751_48145c0d3b21013bd6c2d93094ab9722.pdf
2019-09-01
2619
2624
10.31557/APJCP.2019.20.9.2619
Hyperglycemia
ALL induction phase
steroid
L-asparaginase
Mururul
Aisyi
aci97nabil@yahoo.com
1
Department of Pediatric Hematology Oncology, Indonesian National Cancer Center “Dharmais” Cancer Hospital, Jakarta, Indonesia.
LEAD_AUTHOR
Murti
Andriastuti
murtiandri@yahoo.com
2
Department of Child Health, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
AUTHOR
Nia
Kurniati
niawidj@gmail.com
3
Department of Child Health, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
AUTHOR
ORIGINAL_ARTICLE
Role of CD135/CD117 on Prognosis and Overall Survival of Acute Myeloid Leukemia
Background: The key proliferative RTKs for AML include c-KIT receptor (CD117) and FLT-3 receptor (CD135).The aim of this study was to evaluate the CD135 and CD117 expression, co-expression of CD135 and CD117(CD135+CD117), and the association of that co-expression with Event Free Survival (DFS) and Overall Survival (OS)rates. Material and Methods: We analyzed CD117 and CD135 expression on AML blasts by flow cytometry and itsassociation with Event Free Survival (DFS) and Overall Survival (OS) in 66 AML treated on Hematology-OncologyResearch Center, Iran, Tabriz. Results: The overall OS and EFS were 50% and 80.3% respectively during our study.Cox-regression analysis revealed that a poor EFS was significantly associated a low CD135 (HR 0.34, 95% CI0.13–0.88, P = 0.02). Conclusion: This is the first study from Iran to show that the expressions of CD135, CD117 iseasily measurable by routine diagnostic flow-cytometry, and CD135+117 were not significantly associated with CR,EFS, or OS .
https://journal.waocp.org/article_88716_19bc8696ef67c2980ad4dd12f388c879.pdf
2019-09-01
2625
2631
10.31557/APJCP.2019.20.9.2625
acute myeloid leukemia
CD135
CD117
Flow-cytometry
Mortaza
Raeisi
raiisy@yahoo.com
1
Tabriz University of Medical Sciences, Tabriz, Iran.
AUTHOR
Ali Reza
Nikhanfar
nikhanfarar@hotmail.com
2
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
AUTHOR
Babak
Nejate
babaknejati88@gmail.com
3
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
AUTHOR
Ali Akabr
Movassaghpour Akbari
movassaghpour@gmail.com
4
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
AUTHOR
Roya
Dolatkhah
royadolatkhah@yahoo.com
5
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
AUTHOR
Yousef
Roosta
yroosta@gmail.com
6
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
AUTHOR
Zohreh
Sanaat
zohrehsanaat@yahoo.com
7
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Effect of Pentoxifylline on Staurosporine-Induced Neurite Elongation in PC12 Cells
Objective: Pentoxifylline enhances neurite elongation in PC12 cells. This study investigated the effects of pentoxifylline on staurosporine-induced neurite elongation in PC12 cells. Materials and Methods: There were five treatment groups, including treatment group I (1 nM), treatment group II (10 nM), treatment group III (100 nM), treatment group IV (1uM), and treatment group V (10 mM of pentoxifylline), together with 214 nM staurosporine for a range of time (6, 12 and 24 hours). Cells only treated with staurosporine at a concentration of 214 nM were used as the control group. Cell proliferation, cell death, immunocytochemistry assay, and Total Neurite Length were assessed. Results: The results showed that pentoxifylline increased cell viability (p<0.05) in a dose- and time-dependent manner, and cell death assay showed that cell death decreased in a dose- and time-dependent manner (p<0.05). TNL increased significantly compared with control cells (p<0.05). Immunocytochemistry assay showed that pentoxifylline at low and high concentrations enhanced β-tubulin III and GFAP protein expression compared with control cells. Conclusion: It can be concluded that pentoxifylline has positive effects on the staurosporine-induced neurite outgrowth process in PC12 cells.
https://journal.waocp.org/article_88717_a7c6332218503da89e1a00c1c2765411.pdf
2019-09-01
2633
2638
10.31557/APJCP.2019.20.9.2633
pentoxifylline
cell death
Neurite
staurosporine
Loghman
Diojan
zagrosdio@gmail.com
1
Department of Radiology and Nuclear Medicine, School of Allied Medical Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran.
AUTHOR
Hossein
Zhaleh
hos.zhale@gmail.com
2
Substance Abuse Prevention Research Center, Institute of Health, Kermanshah University of Medical Sciences, Kermanshah, Iran.
AUTHOR
Mehri
Azadbakht
azadbakht.tm@gmail.com
3
Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran.
AUTHOR
Ali
Bidmeshkipour
al.bidmeshki@gmail.com
4
Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran.
AUTHOR
Ehsan
Khodamoradi
eh_medph@yahoo.com
5
Department of Radiology and Nuclear Medicine, School of Allied Medical Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran.
LEAD_AUTHOR
ORIGINAL_ARTICLE
The Comparison of the Plasma Levels of the Lead Element in Patients with Gastrointestinal Cancers and Healthy Individuals
Back ground and Aim: Heavy metals are considered as risk factors in the development of some types of cancers. In this context, the lead (Pb) along with its biological impacts on the human body has raised significant concerns in public health. The aim of this study was to compare the plasma levels of the lead element in patients with gastrointestinal (GI) cancer and healthy subjects to examine whether this element has a role in the susceptibility of cancer. Methods: In a case-control study conducted between March 2016 to February 2017, the plasma levels of the lead were assessed. One-hundred patients with upper and lower GI cancers, as well as one-hundered healthy subjects who were age- and sex-matched participated in our study. A classic flame atomic absorption spectroscopy (FAAS) method was employed for the determination of the lead element in plasma levels of all subjects. Results: The mean age of patients was 53.8±10.6 years old. The patient group consisted of 51 male and 49 female patients. The results showed that the concentrations of Pb were lower than the defined toxic levels. The comparison of the mean levels of Pb between the case and control groups revealed that there was no statistically significant difference even when the gender, age, and history of smoking were included in the statistical analysis. Our findings showed that the concentration of Pb is significantly associated with the type of cancer (p<0.003) and the location of the tumor (whether upper or lower tract was affected) (p<0.003). Conclusion: Lead may contributes to the pathology and progression of GI cancers but we can not conclude that it involved in the causation or susceptibility of healthy individuals to develop GI cancer.
https://journal.waocp.org/article_88728_c95b5ae1ff366a48972b3e8e64b05194.pdf
2019-09-01
2639
2644
10.31557/APJCP.2019.20.9.2639
Lead
cancer
Gastrointestinal
trace elements
Masoudreza
Sohrabi
sohrab_r@yahoo.com
1
Gastrointestinal and Liver Diseases Research Center (GILDRC), Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.
AUTHOR
Zahedine
Kheiri
kheyri.zahedin@yahoo.com
2
Department of Internal Medicine, Arak University of Medical Sciences, Arak, Iran.
AUTHOR
Ali
Gholami
aagholami80@yahoo.com
3
3Noncommunicable Diseases Research Center, Neyshabur University of Medical Sciences, Neyshabur, Iran.
AUTHOR
Mehran
Haghighi
mehran.haghighi@gmail.com
4
Gastrointestinal and Liver Diseases Research Center (GILDRC), Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.
AUTHOR
Fahimeh
Safarnezhad Tameshkel
fahime.1615@yahoo.com
5
Gastrointestinal and Liver Diseases Research Center (GILDRC), Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.
AUTHOR
Mahmood
Khoonsari
6
Gastrointestinal and Liver Diseases Research Center (GILDRC), Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.
AUTHOR
Majidreza
Adelani
koh.z@yahoo.com
7
Gastrointestinal and Liver Diseases Research Center (GILDRC), Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.
AUTHOR
Amirhossein
Mirhosseini
8
Gastrointestinal and Liver Diseases Research Center (GILDRC), Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.
AUTHOR
Melika
Sohrabi
malikhah@hotmail.com
9
Department of Analytic Chemistry, Faculty of Basic Sciences, Tehran Islamic Azad University, North Branch, Tehran, Iran.
AUTHOR
Azam
Rezaei Farimani
salehpoor.d@yahoo.com
10
Department of Basic Medical Science, Neyshabur University of Medical Sciences, Neyshabur, Iran.
AUTHOR
Farhad
Zamani
articlesubmitmed@yahoo.com
11
Gastrointestinal and Liver Diseases Research Center (GILDRC), Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.
AUTHOR
Hossein
Ajdarkosh
ajdarkosh1345@yahoo.com
12
Gastrointestinal and Liver Diseases Research Center (GILDRC), Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.
AUTHOR
Amir Hossein
Faraji
amirfaraji1350@gmail.com
13
Gastrointestinal and Liver Diseases Research Center (GILDRC), Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran.
LEAD_AUTHOR
ORIGINAL_ARTICLE
In Vivo Anti Cancer Potential of Pyrogallol in Murine Model of Colon Cancer
Background: Colon cancer is aggressive and it causes 0.5 million deaths per year. Practicing natural medicinesfor cancer treatment is safer than conventional drugs. World health organization emphasizes on the importance ofpracticing natural medicines and developing natural product based drugs for cancer treatment. Recently we reportedan anti colon cancer activity associated with pyrogallol isolated from medicinal plant Acacia nilotica in HT-29 cells invitro. To extend our observation in this study we evaluated in vivo colon tumor remission property of acetone extractof A. nilotica (ACE) and pyrogallol. Materials and Methods: In vivo toxicity of ACE and pyrogallol was assessed andIn vivo tumor remission activity of ACE and pyrogallol was determined in murine model. Results: Mice were tolerateddifferent doses of ACE and pyrogallol. Tumor size was considerably reduced in pyrogallol treated mice similar todoxorubicin. Tumor bearing mice treated with ACE and pyrogallol showed mild decline in body weight. Conclusion:Pyrogallol was found to be an effective anti colon cancer agent with less toxicity.
https://journal.waocp.org/article_88713_82fdc64a5bae2ab65e0e7e20eeb9c621.pdf
2019-09-01
2645
2651
10.31557/APJCP.2019.20.9.2645
Colon cancer
Acacia nilotica
Pyrogallol
Helicobacter pylori
Toxicity
Seemaisamy
Revathi
srevathi155@gmail.com
1
Department of Zoology, Periyar University, Salem 636 011, Tamil Nadu, India.
AUTHOR
Faruck Lukmanul
Hakkim
clonehakkim@gmail.com
2
Department of Mathematics and Sciences, College of Arts and Applied Sciences, , Dhofar University, Salalah, Oman.
LEAD_AUTHOR
Neelamegam
Ramesh Kumar
neelamegamramesh@gmail.com
3
Insect Molecular Biology Laboratory, Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
AUTHOR
Hamid A
Bakshi
hamid.bakshi@gmail.com
4
School of Pharmacy and Pharmaceutical Sciences, Saad Centre for Pharmacy and Diabetes, Ulster University, Cromore Road, Coleraine, Co. Londonderry, BT52 1SA, United Kingdom.
AUTHOR
Alagar Yadav
Sangilimuthu
smuthu.al@gmail.com
5
Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, India.
AUTHOR
Murtaza M
Tambuwala
m.tambuwala@ulster.ac.uk
6
School of Pharmacy and Pharmaceutical Sciences, Saad Centre for Pharmacy and Diabetes, Ulster University, Cromore Road, Coleraine, Co. Londonderry, BT52 1SA, United Kingdom.
AUTHOR
Mohammad
Changez
changejm12@gmail.com
7
Chemistry Division, Department of Basic Sciences, College of Applied and Health Sciences, Sharqiyah University, Ibra, Oman.
AUTHOR
Mohamed M
Nasef
mohamed.nasef@hud.ac.uk
8
Department of Pharmacy, School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield, United Kingdom.
AUTHOR
Muthukalingan
Krishnan
profmkrish@gmail.com
9
Insect Molecular Biology Laboratory, Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
AUTHOR
Nagarajan
Kayalvizhi
kayalvizhinagarajan@gmail.com
10
Department of Zoology, Periyar University, Salem 636 011, Tamil Nadu, India.
AUTHOR
ORIGINAL_ARTICLE
Can Chemotherapy be Integrated with Brachytherapy in Locally Advanced Carcinoma Cervix- A Proof of Principle Study
Introduction: Cancer of the cervix is the second most common cancer in women in India. Chemoradiotherapy is the standard treatment for locally advanced carcinoma cervix. Chemotherapy is not given on days of brachytherapy due to the fear of increased toxicity though studies supporting or refuting it are limited. We intended to study feasibility of adding chemotherapy to brachytherapy with assessment of acute toxicity and response rates. Methods: 29 patients of locally advanced carcinoma cervix (FIGO IIB to IIIB) were assigned to receive either three sessions of high dose rate (HDR) brachytherapy alone or HDR brachytherapy with concurrent chemotherapy of Paclitaxel and Carboplatin after completion of external beam radiation with concurrent Cisplatin. Patients were assessed for compliance of treatment, toxicity and response rates at three and six months. The p-value less than 0.05 was considered statistically significant. Fischer’s exact test was used for statistical analysis. Results: 15 patients were assigned to the standard of care arm and 14 patients to the experimental chemo-brachytherapy arm. The median number of cycles of chemotherapy possible with brachytherapy was two (Range: 1 -3). At three months after treatment all patients except one patient in each arm had a complete response. There was two acute grade 3 hematological toxicity and two acute grade 3 or higher gastrointestinal toxicity in the experimental arm but none in the standard arm. The experimental arm had a statistically higher incidence of acute grade 3 and 4 toxicity than the standard arm (p=0.042). Conclusions: Chemo-brachytherapy is associated with higher acute toxicity with comparable response rates. Small patient numbers and short follow up impedes us from providing conclusive evidence.
https://journal.waocp.org/article_88756_1988df44123b22ac49504ca965a57f34.pdf
2019-09-01
2653
2657
10.31557/APJCP.2019.20.9.2653
Carcinoma cervix
High dose rate brachytherapy
Chemo-brachytherapy
Subhash
Gupta
drsubhashgupta72@gmail.com
1
Department of Radiation Oncology, All India Institute of Medical Sciences, New Delhi, India.
AUTHOR
Prashanth
Giridhar
prashanth.jipmer@gmail.com
2
Department of Radiation Oncology, All India Institute of Medical Sciences, New Delhi, India.
LEAD_AUTHOR
Dayanand
Sharma
sharmadnaiims@gmail.com
3
Department of Radiation Oncology, All India Institute of Medical Sciences, New Delhi, India.
AUTHOR
Haresh
K P
drkpharesh@gmail.com
4
Department of Radiation Oncology, All India Institute of Medical Sciences, New Delhi, India.
AUTHOR
Julka
P K
prashanth.aiims@gmail.com
5
Department of Radiation Oncology, All India Institute of Medical Sciences, New Delhi, India.
AUTHOR
Goura K
Rath
gkrath2006@gmail.com
6
Department of Radiation Oncology, All India Institute of Medical Sciences, New Delhi, India.
AUTHOR
ORIGINAL_ARTICLE
The Possible Role of Interleukin (IL)-18 and Nitrous Oxide and Their Relation to Oxidative Stress in the Development and Progression of Breast Cancer
Background: Cancer breast is the most common malignant tumor in females globally. Mechanisms linkinginflammatory cytokines and tumour growth and progression have not been established. Interleukin (IL)-18 has amodifying role in the immune defense against tumor cells. It induces production of IFN-γ. It also increases the immunecells cytotoxic activity and enhances the production of other proinflammatory cytokine. Nitric oxide (NO) has bothpromoting and inhibiting effects on tumorigenesis. Oxidative stress is a phenomenon that leads to oxidative damageof biomolecules, mutagenesis and carcinogenesis. Objective: The purpose of this research is to identify the potentialrole of IL18 and NO and their relation to oxidative stress in the development of cancer breast. Patients and Methods:This study included 120 women split into two groups ; control group and patient groups that divided into: group B (30patients with benign breast tumors), group N (30newly diagnosed cancer breast patients) ; and group M (30 metastaticcancer breast patients). Results: Serum total anti-oxidant capacity was significant high in both cancer breast groups.Total oxidative capacity was significantly higher level in metastatic group. NO levels were significantly higher valuesin the three cancer breast patients groups compared to control group.IL18 was significantly high in the metastaticgroup. Conclusions: Serum IL-18 and NO activity can be used as a marker for evaluating disease activity in patientswith cancer breast.
https://journal.waocp.org/article_88747_ab8efe25d641cf85550f8082b528f66c.pdf
2019-09-01
2659
2665
10.31557/APJCP.2019.20.9.2659
Interleukin -18
nitric oxide
breast cancer
oxidative stress
Mona Mohamed K
El-Deeb
mona_moh_eldeeb@yahoo.com
1
Department of Chemical Pathology, Medical Research Institute, Alexandria University, Alexandria, Egypt.
AUTHOR
Heba Gaber
El-Sheredy
heba.gaber99@yahoo.com
2
Department of Cancer Management and Research, Medical Research Institute, Alexandria University, Alexandria, Egypt.
LEAD_AUTHOR
Ayman Farouk
Mohammed
draly71@gmail.com
3
Department of Surgery, Medical Research Institute, Alexandria University, Alexandria, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Exploring the Relationships between Posttraumatic Growth, Wisdom, and Quality of Life in Older Cancer Survivors
Objective: The number of older cancer survivors is steadily rising with a growing aging population, and a greatinterest in evaluating the quality of life is emerged. Although understanding how to improve the quality of life inolder cancer survivors is critical as the number of older survivors continues to grow in communities, little is knownabout empirical evidence regarding predictors of the quality of life in older cancer survivors. This study aimed toexamine relationships between posttraumatic growth, wisdom, and quality of life in older cancer survivors. Methods:A convenience sample of older cancer survivors after completing cancer treatments (n=121) participated from onepublic health center, and they filled out self-report questionnaires on measures of posttraumatic growth, wisdom, andquality of life. Results: As results of multiple regression analysis, the most significant factor on each domain of thequality of life has shown that higher levels of subjective economic status were associated with significant improvement offour domains of quality of life, and wisdom and posttraumatic growth were associated with significant improvement insocial/family well-being. Conclusion: This study highlights predictors of each domain of quality of life that subjectiveeconomic status, posttraumatic growth and wisdom significantly affected the quality of life in older cancer survivors.Findings indicate that psychological interventions need to be developed and implemented for older cancer survivorsto prevent long-term effects of cancer and to increase their quality of life. For improving their quality of life, primarycare providers or community health professionals need to develop tailored interventions, such as home-based cancersurvivorship programs.
https://journal.waocp.org/article_88729_20776bbc7333257e196d1fd7ce423b6c.pdf
2019-09-01
2667
2672
10.31557/APJCP.2019.20.9.2667
Posttraumatic growth
wisdom
Quality of Life
Elderly
cancer survivor
Seung-Kyoung
Yang
yangsk@kyungnam.ac.kr
1
Department of Nursing, Kyungnam University, Changwon, South-Korea.
AUTHOR
Yeongmi
Ha
yha@gnu.ac.kr
2
College of Nursing and Institute of Health Sciences, Gyeongsang National University, Jinju, South-Korea.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Late Effects of Breast Cancer Treatment and Outcome after Corrective Interventions
Purpose: To study the late toxicities of treatment and its impact on Breast cancer survivors among Indian patients.Materials and Methods: Our study recruited 152 curatively treated non metastatic carcinoma breast patients. Thebaseline demographic details, disease related and treatment related information were collected. The late effects includedbreast cancer related lymphedema, shoulder dysfunction, treatment induced bone loss, hypothyroidism, cardiacdysfunction, and chemotherapy induced cognitive dysfunction and Quality of life. Results: The median age was 47years (range 27 -72 years). The cumulative frequency of BCRL and shoulder dysfunction was 31.57% and 34.86%respectively. The improvement in BCRL with corrective intervention was not statistically significant. The BCRL wassignificantly associated with shoulder dysfunction. The frequency of loss of bone mineral density was 38.15%. There wasstatistically significant improvement in bone mineral density with interventions. The cumulative rate of hypothyroidismand cardiac dysfunction was 14.47 % and 2.17% respectively which improved after corrective therapy. We did not findany delayed cognitive dysfunction. There was improvement in global health, physical function, role function, fatigue,Nausea, vomiting, pain scores, insomnia, Loss of appetite, diarrhea and arm symptoms over time with intervention.Conclusion: Our study has shown that nearly half of the survivors were suffering from at least one of the late effects.The intervention helped in improving the loss of bone mineral density, hypothyroidism, cardiac dysfunction and qualityof life in Breast cancer survivors.
https://journal.waocp.org/article_88740_6521514a1a22bd3496bb1c5112349a1a.pdf
2019-09-01
2673
2679
10.31557/APJCP.2019.20.9.2673
Late effects
breast cancer
Quality of Life
Unni S
Pillai
druspillai@gmail.com
1
Department of Medical Oncology, Jawaharlal Institute of Post Graduate Medical Education & Research (JIPMER), Puducherry, India.
AUTHOR
Smita
Kayal
kayalsmita@gmail.com
2
Department of Medical Oncology, Jawaharlal Institute of Post Graduate Medical Education & Research (JIPMER), Puducherry, India.
AUTHOR
Sunu
Cyriac
drsunucyraic@gmail.com
3
Department of Medical Oncology, Jawaharlal Institute of Post Graduate Medical Education & Research (JIPMER), Puducherry, India.
AUTHOR
Yadav
Nisha
yadav.nisha250@gmail.com
4
Department of Medical Oncology, Jawaharlal Institute of Post Graduate Medical Education & Research (JIPMER), Puducherry, India.
AUTHOR
Kadambari
Dharanipragada
d_kadambari@yahoo.com
5
Department of Surgery, Jawaharlal Institute of Post Graduate Medical Education & Research (JIPMER), Puducherry, India.
AUTHOR
Sadish Kumar
Kamalanathan
sadishkk@gmail.com
6
Department of Endocrinology, Jawaharlal Institute of Post Graduate Medical Education & Research (JIPMER), Puducherry, India.
AUTHOR
Dhanapathi
Halanaik
dhanapathih@gmail.com
7
Department of Nuclear Medicine,Jawaharlal Institute of Post Graduate Medical Education & Research (JIPMER), Puducherry, India.
AUTHOR
Navin
Kumar
drnavin_agarwal@rediffmail.com
8
Department of Physiology, Jawaharlal Institute of Post Graduate Medical Education & Research (JIPMER), Puducherry, India.
AUTHOR
Ponraj
Madasamy
ponsmadasamy@gmail.com
9
Department of Medical Oncology, Jawaharlal Institute of Post Graduate Medical Education & Research (JIPMER), Puducherry, India.
AUTHOR
Dhanraju Krishnappa
Muniswamy
dhannuenator@gmail.com
10
Department of Medical Oncology, Jawaharlal Institute of Post Graduate Medical Education & Research (JIPMER), Puducherry, India.
AUTHOR
Biswajit
Dubashi
drbiswajitdm@gmail.com
11
Department of Medical Oncology, Jawaharlal Institute of Post Graduate Medical Education & Research (JIPMER), Puducherry, India.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Identification of Potent VEGF Inhibitors for the Clinical Treatment of Glioblastoma, A Virtual Screening Approach
Vascular endothelial growth factor (VEGF) expression could be found in all glioblastomas. VEGF takes part in numerous changes including the endothelial cell proliferation, the vasculature of solid tumor: its survival invasion, and migration, chemotaxis of bone marrow-derived progenitor cells, vasodilation and vascular permeability. VEGF inhibition can be a smart therapeutic strategy because it is extremely specific and less toxic than cytotoxic therapy. To establish better inhibition of VEGF than the current inhibitors, present study approach is by molecular docking, virtual screening to illustrate the inhibitor with superior affinity against VEGF to have a cautious pharma profile. To retrieve the best established and high-affinity high affinity molecule, Molegro Virtual Docker software was executed. The high-affinity scoring compounds were subjected to further similarity search to retrieve the drugs with similar properties from pubchem database. The completion of virtual screening reveals that PubChem compound SCHEMBL1250485 (PubChem CID: 66965667) has the highest affinity. The study of the drug-likeness was verified using OSIRIS Property Explorer software which supported the virtual screened result. Further ADMET study and drug comparative study strongly prove the superiority of the new established inhibitor with lesser rerank score and toxicity. Overall, the new inhibitor has higher potential to stop the expression of VEGF in glioblastoma and positively can be further analysed through In vitro studies.
https://journal.waocp.org/article_88742_9b5e21c7ba780f2a745ae6e064ca75b4.pdf
2019-09-01
2681
2692
10.31557/APJCP.2019.20.9.2681
VEGF
Glioblastoma
Virtual screening
Molecular docking
Mohini
Yadav
mohini.y@eminentbio.com
1
In silico Research Laboratory, Eminent Biosciences, Indore – 452 010, Madhya Pradesh, India.
AUTHOR
Ravina
Khandelwal
ravina.k@eminentbio.com
2
In silico Research Laboratory, Eminent Biosciences, Indore – 452 010, Madhya Pradesh, India.
AUTHOR
Urvy
Mudgal
urvymudgal22@gmail.com
3
In silico Research Laboratory, Eminent Biosciences, Indore – 452 010, Madhya Pradesh, India.
AUTHOR
Sivaraj
Srinitha
srinitha.s@eminentbio.com
4
In silico Research Laboratory, Eminent Biosciences, Indore – 452 010, Madhya Pradesh, India.
AUTHOR
Natasha
Khandekar
natasha.k@eminentbio.com
5
In silico Research Laboratory, Eminent Biosciences, Indore – 452 010, Madhya Pradesh, India.
AUTHOR
Anuraj
Nayarisseri
anuraj@eminentbio.com
6
In silico Research Laboratory, Eminent Biosciences, Indore – 452 010, Madhya Pradesh, India.
LEAD_AUTHOR
Sugunakar
Vuree
sugunakar.v@eminentbio.com
7
Department of Biotechnology, Lovely Faculty of Technology and Sciences, Division of Research and Development, Lovely Professional University, Phagwara, Punjab-144411, India.
AUTHOR
Sanjeev Kumar
Singh
sanjeev.s@eminentbio.com
8
Computer Aided Drug Designing and Molecular Modeling Lab, Department of Bioinformatics, Alagappa University, Karaikudi-630 003, Tamil Nadu, India.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Impact of Early Dressing Removal on Tunneled Central Venous Catheters: A Piloting Study
Background: Central Venous Catheters (CVC) are linked with Catheter-related bloodstream infections (CLABSI)or exit-site infections. Dressings may reduce the rate of infection, but they are uncomfortable, do not eliminate therisk of infection, and in some cases become the cause of infection. Aim: This study evaluates the impact of earlyCVC dressing removal on CLABSI, exit-site infections, and patient quality of life in an oncology setting. Method: Aquasi-experimental pilot study was conducted over 15 months at a specialized oncology center. Sixteen patients weredivided into control (n=8) and experimental (n=8) groups. The control group received the standard protocol of applyingCVC dressings, while the experimental group received a “no-dressing” protocol. Results: There was no statisticalsignificance in the infection rate between the two groups (p=1.0). Two cases developed CLABSIs, one in each group.One patient from the experimental group developed an exit-site infection as well. Patients in the experimental groupreported high satisfaction and an improved quality of life. Conclusions: Applying a no-dressing protocol to a wellhealedexit site CVC showed encouraging results in terms of exit-site and bloodstream infections. That is to say; itdid not predispose patients to increased risk of infections. Furthermore, patients with no dressing protocol feel morecomfortable in their life.
https://journal.waocp.org/article_88741_11e75dea05e05561eaf89e3c7eb6440c.pdf
2019-09-01
2693
2697
10.31557/APJCP.2019.20.9.2693
Keywords: Tunnel Central Venous Catheter
no-dressing
well-healed exit site
Ghada
Ammar
gammar@khcc.jo
1
King Hussein Cancer Center, Jordan.
LEAD_AUTHOR
Ezzaldeen
Almashaikh
emashaikh@kcc.jo
2
King Hussein Cancer Center, Jordan.
AUTHOR
Ahmad
Ibdah
aibdah@khcc.jo
3
King Hussein Cancer Center, Jordan.
AUTHOR
Waleed
Shajrawi
wshajrawi@khcc.jo
4
King Hussein Cancer Center, Jordan.
AUTHOR
Safwat
Awawdeh
sawawdeh@khcc.jo
5
King Hussein Cancer Center, Jordan.
AUTHOR
Ayoub
AL Mousa
aamousa@khcc.jo
6
King Hussein Cancer Center, Jordan.
AUTHOR
Belal
AL-Blowi
bbalwi@khcc.jo
7
King Hussein Cancer Center, Jordan.
AUTHOR
Moh’d Baseem
Hamdan
bhamdan@khcc.jo
8
King Hussein Cancer Center, Jordan.
AUTHOR
Amani
Al Eleiwah
aa.11338@khcc.jo
9
King Hussein Cancer Center, Jordan.
AUTHOR
Wala
Al Jabali
wa.10820@khcc.jo
10
King Hussein Cancer Center, Jordan.
AUTHOR
Hussien
Hussien
hmhussien@khcc.jo
11
King Hussein Cancer Center, Jordan.
AUTHOR
Abdelrahman
Salameh
abdelrahmansalameh@gmail.com
12
King Hussein Cancer Center, Jordan.
AUTHOR
Mohammad
Alkharabsheh
13
King Hussein Cancer Center, Jordan.
AUTHOR
ORIGINAL_ARTICLE
Stage-Specific Survival Rate of Breast Cancer Patients in Northern Thailand in Accordance with Two Different Staging Systems
Objective: This study was attempted to investigate overall survival by stage at diagnosis in female breast cancerpatients in Northern Thailand by using 2 different staging systems; namely, American Joint Committee on Cancer (AJCC)Tumor (T), Nodal (N) and Metastatic (M) staging system and Surveillance Epidemiology and End Results (SEER)summary staging system. Methods: We studies female breast cancer patients whose data were registered in Chiang Maicancer registries between January 2006 and December 2015. Data were recorded in SEER summary staging system.The TNM AJCC staging was searched in the medical records. Results: A total of 3,873 female breast cancer patientswere diagnosed from 2006-2015. All data sets were recorded in SEER summary stage 2000. Early stage was the mostprevalent stage at the time of diagnosis (58%), followed by loco-regional advanced stage (32%), and metastatic breastcancer (10%). The 5-year overall survival rate of early, loco-regional advanced, and metastatic stages were 85.3%,66.4%, and 26.2%, respectively. After examining the medical records, we excluded patients who had no data on T, N,and M in their records. Finally, only 3,251 patients were analyzed for AJCC stage-specific survival. The 5-year overallsurvival rate in stages I, II, III, and IV were 94.4%, 85.0%, 56.6%, and 28.3%, respectively. Conclusion: Comparingto more stable economic countries, the 5-year overall survival rate for a specific stage of breast cancer in NorthernThailand was slightly lower in early stage and stage I-II in accordance with AJCC, but much lower in loco-regional stageand stage III with respect to AJCC. Nevertheless, it was similar in metastatic stage and stage IV according to AJCC.
https://journal.waocp.org/article_88734_227e26fe42b45d73ac8ec1d8bf1cccfc.pdf
2019-09-01
2699
2706
10.31557/APJCP.2019.20.9.2699
breast cancer
Overall survival
AJCC staging
SEER staging
Imjai
Chitapanarux
imjai@hotmail.com
1
Division of Radiation Oncology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
LEAD_AUTHOR
Patumrat
Sripan
pspatumrat3@gmail.com
2
Division of Radiation Oncology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
AUTHOR
Areewan
Somwangprasert
asomwang@yahoo.com
3
Department of Surgery, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
AUTHOR
Chaiyut
Charoentum
ccharoentum@gmail.com
4
Oncology Unit, Department of Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
AUTHOR
Wimrak
Onchan
ai_name@hotmail.com
5
Division of Radiation Oncology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
AUTHOR
Kirati
Watcharachan
kvatchara@yahoo.com
6
Department of Surgery, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
AUTHOR
Panchaporn
Wongmaneerung
lleb_pn@hotmail.com
7
Department of Surgery, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
AUTHOR
Pailin
Kongmebhol
mailtoann@gmail.com
8
Department of Radiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
AUTHOR
Bongkot
Jia-Mahasap
phung_nemo@hotmail.com
9
Division of Radiation Oncology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
AUTHOR
Lalita
Huntrakul
jip_lalit@hotmail.com
10
Department of Radiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
AUTHOR
ORIGINAL_ARTICLE
Genetic Polymorphisms of Xenobiotic Metabolizing Genes (GSTM1, GSTT1, GSTP1), Gene-Gene Interaction with Association to Lung Cancer Risk in North India; A Case Control Study
Aim: In this case control study involving, 220 human subjects; polymorphisms in xenobiotic metabolizing genes (GST-M1, -T1 and -P1) and their association to lung cancer risk is being analysed among smokers and non-smokers. GSTM1 or GSTT1 gene polymorphism and amino acid changes in GSTP1 have been correlated and may be associated to lung cancer risk. Other factor includes exposure to environmental pollutants and life style choices. We have explored gene-gene and gene-environment interaction in the aetiology of lung cancer risk among north Indian population. Patients and Methods: For the study we have collected 120 lung cancer patient blood samples from Kamala Nehru Memorial Cancer Hospital, Allahabad, Uttar Pradesh and 100 matched controls. DNA was isolated and GST-M1 and - T1 genotyping were assessed by multiplex PCR whereas the GSTP1 polymorphism was analysed using restriction fragment length polymorphism. The risk of lung carcinogenesis was assessed using logistic regression analysis calculating the odd ratio (OR) with 95% confidence interval (CI). Results: The risk of lung carcinogenesis was three fold higher for null GSTT1 (OR=3.045, 95%CI=1.750-5.301, p-value <0.001) genotype; whereas other two types; GSTM1 (OR= 1.342, 95% CI=0.788-2.284, p-value=0.270) and GSTP1 (OR=0.806, 95% CI=0.526-1.236, p-value=0.323) showed no association to lung cancer susceptibility respectively. Smokers diagnosed with lung cancer had more null genotypes for GSTT1 (OR=4.773, 95%CI=1.939-11.751, p<0.001). The ‘at risk’ genotype combination GSTM1 (null) /GSTT1 (null) (OR=1.76, 95%CI; 0.920-3.370, p-value=0.03) showed increased susceptibility to lung cancer risk. The genotype combination of GSTT1 (null)/GSTP1 (Ile/Ile) (p=0.009) was associated with increased lung cancer risk. Conclusion: The results of this study suggest that; GSTT1 null genotype were more susceptible for lung cancer risk and smoking increases the susceptibility for lung cancer several folds among the North Indian population. Gene-gene interaction for null genotypes of GSTM1 and GSTT1 were correlated with higher risk of having lung cancer.
https://journal.waocp.org/article_88724_4784ad4e4c971152e20bd4ca67c3ad6c.pdf
2019-09-01
2707
2714
10.31557/APJCP.2019.20.9.2707
Polymorphism
GSTT1
GSTM1
GSTP1
Lung cancer
.
Ritambhara
ritambhara.vns@gmail.com
1
Department of Biochemistry, University of Allahabad, Allahabad, UP-211002, India.
AUTHOR
Sonia
Tiwari
tiwarisonia05@gmail.com
2
Department of Radiation Oncology, Kamala Nehru Memorial Hospital, Allahabad, UP-211002, India.
AUTHOR
Sivakumar
Vijayaraghavalu
livshiva@gmail.com
3
Central Research Facility, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University), Porur, Chennai, 600116, Tamil Nadu, India.
AUTHOR
Munish
Kumar
munishkp@gmail.com
4
Department of Biochemistry, University of Allahabad, Allahabad, UP-211002, India.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Reduced Expression of IL-1β and IL-18 Proinflammatory Interleukins Increases the Risk of Developing Cervical Cancer
Background: The objective of this study was to analyze the gene expression profile of the proinflammatoryinterleukins, (IL-1β and IL-18) in patients with premalignant lesions and cervical cancer. Methods: Total IL-1β andIL-18 mRNA was quantified by qPCR to obtain the expression data in cervical tissues. A total of 74 cervical biopsieswere obtained from women undergoing a colposcopy. The samples were divided into: normal (19), low level lesions(LSIL) or NIC I (17), high level lesions (HSIL) or CIN II and CIN III (29) and cancer (9). The normal cervical tissuesamples were included as controls. The OR and 95% CI were calculated for the determination of the risk of progressionbetween each type of lesion and cancer using logistic regression. Results: The results showed that an increase in therisk of progression of pre-neoplastic lesions to cancer was between 2.5 and 2.08 times higher in women with lowerIL-1β and IL-18 expression, respectively. Conclusions: This study provided evidence that IL-1β and IL-18 are potentialbiomarkers that can be explored in further studies for monitoring the evolution of pre-neoplastic lesions and avoidingovertreatment or undertreatment of the patients.
https://journal.waocp.org/article_88730_cba5b0134693569222fa8e2b9592a850.pdf
2019-09-01
2715
2721
10.31557/APJCP.2019.20.9.2715
Cervical cancer
HPV
IL-18
IL-1β
Biomarker
Jose Anibal
Matamoros
anibalmatamoros@gmail.com
1
Laboratory of Molecular Biology of Viruses, Biological Sciences Institute, University of Pernambuco, Brazil.
AUTHOR
Maria Isabel Ferreira
da Silva
maria95xd@hotmail.com
2
Laboratory of Molecular Biology of Viruses, Biological Sciences Institute, University of Pernambuco, Brazil.
AUTHOR
Patrícia Muniz Mendes Freire
de Moura
patricia.moura@upe.br
3
Laboratory of Molecular Biology of Viruses, Biological Sciences Institute, University of Pernambuco, Brazil.
AUTHOR
Maria da Conceição Gomes
Leitão
mcgomesl@yahoo.com.br
4
Laboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Biological Sciences Center, Federal University of Pernambuco, Recife, Brazil.
AUTHOR
Eliane Campos
Coimbra
elianeccoimbra@gmail.com
5
Laboratory of Molecular Biology of Viruses, Biological Sciences Institute, University of Pernambuco, Brazil.
LEAD_AUTHOR
ORIGINAL_ARTICLE
MicroRNA-215 as a Diagnostic Marker in Egyptian Patients with Hepatocellular Carcinoma
Background: MicroRNAs are mentioned as a small non-coding RNAs groups and aberrant miRNA expression wasfound in hepatocellular carcinoma (HCC) patients. Aim: To evaluate role of plasma MicroRNA-215 as a diagnostictool in HCC patients. Methods: A prospective study included 195 subjects: healthy controls (group I), cirrhotic patients(group II), and patients with HCC (group III). Clinical examination, radiological and laboratory investigations whichincluded quantification of miR-215 by Real-time qPCR were done for all cases. Results: Spearman’s rank correlationrevealed that in HCC group, there was a negative correlation between MiRNA-215 and serum AFP levels and focal sizelesion (cm) (rs = -0.72, - 0.94 respectively, p<0.001). Receiver operating characteristics analysis for discriminationbetween cirrhosis and HCC groups regarding microRNA-215 displayed 78.3% sensitivity, 88.0% specificity at cutoffvalue of ≤ 1.90. Area under the curve (AUC) was 0.87 (p< 0.001). As regards AFP, it had a sensitivity of 81.7%, aspecificity of 66.7 at cutoff value of ≥ 11.50 (ng/mL). Conclusions: Plasma level of miR-215 may be a promisingbiomarker in HCC diagnosis. Moreover, if miR-215 combined with AFP, it can be used as a diagnostic biomarker, forearly detection of HCC.
https://journal.waocp.org/article_88719_f1db4ac5c86ee471326d39c93e25a7e5.pdf
2019-09-01
2723
2731
10.31557/APJCP.2019.20.9.2723
hepatocellular carcinoma (HCC)
chronic hepatitis C virus (chronic HCV)
MicroRNA-215 (miR-215)
Hussein Ahmed
El Mahdy
elmahdyhussein@gmail.com
1
Department of Biochemistry, Faculty of Science, Cairo University, Egypt.
AUTHOR
Ismail Abdelshafy
Abdelhamid
ismail_shafy@yahoo.com
2
Department of Chemistry, Faculty of Science, Cairo University, Egypt
AUTHOR
Ahmed Ibrahim
Amen
drahmed2008_1210@yahoo.com
3
Department of Biochemistry, Faculty of Science, Cairo University, Egypt.
AUTHOR
Eman
Abdelsameea
eabdelsameea@liver-eg.org
4
Department of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Egypt.
LEAD_AUTHOR
Mona M
Hassouna
ysahassouna@yahoo.com
5
Department of Clinical pathology, National Liver Institute, Menoufia University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
A Randomized, Open-Labeled, Prospective Controlled Study to Assess the Efficacy of Frontline Empirical Intravenous Piperacillin/Tazobactam Monotherapy in Comparison with Ceftazidime Plus Amikacin for Febrile Neutropenia in Pediatric Oncology Patients
Background: Febrile neutropenia (FN) is the most common complication in pediatric oncology patients. Appropriate empirical antibiotics treatment is essential for treatment outcome. Methods: This study was a randomized prospective controlled study to demonstrate the efficacy of piperacillin/tazobactam (PIP/TZO) monotherapy compared with ceftazidime/amikacin in children with FN. Pediatric oncology patients at Chiang Mai University Hospital, diagnosed with FN, were randomized to receive either PIP/TZO 320 mg/kg/day divided every 8 hours or ceftazidime 100 mg/kg/day divided every 8 hours plus amikacin 15 mg/kg/day once daily. Treatment responses were compared between the two groups. Results: One-hundred and eighteen febrile neutropenic episodes in 70 patients (42 males and 28 females) were enrolled. The median age was 7 (3-10) years. The early response and complete response to initial treatment were achieved in 48/59 (81.4%) episodes and 41/59 (69.5%) episodes in PIP/TZO group compared with 40/59 (67.8%) episodes and 33/59 (55.9%) episodes in ceftazidime/amikacin group (p-value 0.091 and 0.128, respectively). Treatment modification in PIP/TZO group was required in 18/59 (30.5%) compared with 26/59 (44.1%) patients in ceftazidime/amikacin group (p-value 0.128). Similarly, the duration of fever, duration of neutropenia and duration of antibiotics treatment were not significantly different between two groups. No serious adverse events were observed. Conclusion: The treatment responses of PIP/TZO monotherapy and ceftazidime/amikacin therapy were not significantly different. Both therapies were effective for FN in pediatric oncology patients.
https://journal.waocp.org/article_88752_1efadb4e1dc3a181044808df82fd305b.pdf
2019-09-01
2733
2737
10.31557/APJCP.2019.20.9.2733
piperacillin/tazobactam
ceftazidime
amikacin
febrile neutropenia
children
Ruchirek
Kamonrattana
l_mok910@hotmail.com
1
Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
AUTHOR
Lalita
Sathitsamitphong
lalita.sat@cmu.ac.th
2
Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
LEAD_AUTHOR
Worawut
Choeyprasert
worawut.cho@cmu.ac.th
3
Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
AUTHOR
Pimlak
Charoenkwan
pimlak.c@cmu.ac.th
4
Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
AUTHOR
Rungrote
Natesirinilkul
rungrote.n@cmu.ac.th
5
Department of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
AUTHOR
Kanda
Fanhchaksai
iamkanda54@gmail.com
6
Research Cluster of Thalassemia and Red Blood Cell Disorders, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
AUTHOR
ORIGINAL_ARTICLE
Exon1 and -116 C/G Promoter Polymorphism on the X-Box DNA Binding Protein- 1 Gene is not Associated with Breast Cancer among Jordanian Women
Background: Human X -box binding protein 1 (XBP1), a critical gene in the endoplasmic reticulum stress response, islocated on chromosome 22q12, which has been linked with the pathogenesis of many diseases, particularly cancers suchas breast cancer (BC). Single nucleotide polymorphisms (SNPs) in the XBP1 gene can alter structure and function of thegene. In this study, polymorphism in the promoter region and exon1 of the gene XBP1 and its association with BCin Jordanian women was investigated. Methods: Polymorphism in the promoter and exon1 of XBP1 was analyzedin 100 subjects (controls: n=40; BC patients=60). −116 C/G SNP was genotyped by Polymerase Chain Reaction(PCR)-sequence specific primer technique. The odd ratios (ORs) at 95% confidence intervals (CIs) were computedto assess the strength of this association. Results: The three genotypes of the SNP (GG, GC, CC) and their allelicfrequencies have nonsignificant differences between patients and control group. It was noticed that the frequencies ofthe mutant allele (G) were (75.8% versus 24.2%)) in the patients and control groups, respectively, while those of thenormal allele (C) were (67.5% versus 32.5%). XBP1 (-116 G→C) G allele did not show significant association withBC risk (confidence interval = 0.3534- 1.2395, odds ratio = 0.6619, P= 0.197). Moreover, there were no significantmutations in the XBP1 exon1 neither in BC subjects nor control subjects. Conclusions: This is the first study to evaluatethe effect of polymorphism in the promoter and exon1 of XBP1 gene in the pathogenesis of BC in Jordanian women.The results do not support a role for polymorphism in development of BC and further studies with a larger sample sizeand detailed data should be performed in other populations.
https://journal.waocp.org/article_88720_2edcf7f10e6257e345d26c9fbd5fd25c.pdf
2019-09-01
2739
2743
10.31557/APJCP.2019.20.9.2739
−116 C/G polymorphism
breast cancer
SNPs, XBP1 gene
Lulu H
Alsheikh Hussein
1
Department of Biological Sciences, Yarmouk University, Irbid, Jordan.
AUTHOR
Ahmad M
Khalil
kahmad76@yahoo.com
2
Department of Biological Sciences, Yarmouk University, Irbid, Jordan.
LEAD_AUTHOR
Ahmad Y
Alghadi
3
Department of Biological Sciences, Yarmouk University, Irbid, Jordan.
AUTHOR
Abed Alkarem
Abu Alhaija
4
Department of Biological Sciences, Yarmouk University, Irbid, Jordan.
AUTHOR
ORIGINAL_ARTICLE
The Upregulation of OCT4 in Acidic Extracellular pH is Associated with Gemcitabine Resistance in Cholangiocarcinoma Cell Lines
Background: Cholangiocarcinoma (CCA), although is an uncommon liver cancer originating from bile duct epithelial cells, is one of the top 10 most fatal cancers. Chemoresistance is an unmet need always found in CCA patients. Tumor microenvironment conditions such as hypoxia, nutrient starvation and acidic extracellular pH play critical roles in chemoresistance and cancer progression. However, the effect of acidic extracellular pH on cellular response and chemoresistance in CCA has not been studied. Methods: Human CCA cell lines (KKU-M213, KKU-M055 and KKU-100) were cultured under acidic (pH 6.5) or non-acidic (pH 7.4) condition and were used for gene expression, doubling time and cytotoxicity assay. Results: The acidic extracellular pH (pH 6.5) significantly increased doubling times of CCA cell lines compared with non-acidic condition (pH 7.4). Interestingly, extracellular acid condition induced gemcitabine resistance in CCA cell lines. We showed that Octamer-binding transcription factor 4 (Oct4) was upregulated in these cell lines under extracellular acid condition. Conclusion: Our findings demonstrate that CCA cells can adapt to survive in acidic environment after which chemoresistance has been developed. Oct4 may be a key transcriptional regulator which mediates chemoresistance in response to acidic extracellular pH.
https://journal.waocp.org/article_88721_0ff2d84f8a9b48a2df198c767c8b1b59.pdf
2019-09-01
2745
2748
10.31557/APJCP.2019.20.9.2745
Chemoresistance
Tumor Microenvironment
Octamer-binding transcription factor 4
Liver cancer
Cancer stem cell-like cell
Phatchareeporn
Choodetwattana
phatchareeporn.c@gmail.com
1
Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
AUTHOR
Siriporn
Proungvitaya
sirpat@kku.ac.th
2
Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
AUTHOR
Patcharee
Jearanaikoon
patjea@kku.ac.th
3
Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
AUTHOR
Temduang
Limpaiboon
temduang@kku.ac.th
4
Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Circulating miR-34a and miR-125b as Promising non Invasive Biomarkers in Egyptian Locally Advanced Breast Cancer Patients
Background: Breast cancer (BC) is the second most common cancer worldwide. MicroRNAs are a group ofnon-coding, single stranded RNAs of ~ 22 nucleotides, which regulate gene expression at the post-transcriptional level.Circulating miRNAs have been found as potential blood based predictive biomarkers. Purpose: we aim to evaluatemiR-34a and miR-125b to predict outcome from neoadjuvant chemotherapy in Egyptian BC patients. Methodology:Quantitative assessment of plasma miR-34a and miR-125b expression was performed by qRT-PCR. Thirty ninenewly diagnosed locally advanced BC female patients with 10 age and sex matched healthy volunteers were includedin the study. Results: We performed ROC curve analysis to evaluate the diagnostic value for the miR-34a withAUCs = 0.995, cutoff point of 2.57 sensitivity 97.4%, specificity 100%, PPV 100%, NPV 83.3% and accuracy 97.7%.miR-125b had AUC = 0.68 and a cutoff point of 8.69 with sensitivity 66.7%, specificity 70.0%, PPV 90.6%, NPV41.2% and accuracy 73.5%. miR-34a expression were significantly higher in BC patients compared to controls with pvalue <0.001*. Also, miR-34a expression level was significantly higher in patients with progressive disease with P value=0.03*. However, miR-125b expression levels were insignificantly higher in responsive patients with p value = 0.2.Conclusion: miRNAs are crucial candidates for novel molecular targeted therapies due to their capability to regulatenumerous genes in molecular pathways. Our data suggest that circulating miR-34a and miR-125b expression levelscould be promising highly accurate non-invasive biomarkers in diagnosing BCs. miR-34a can predict chemotherapeuticresistance associated with higher expression levels in non-responsive patients.
https://journal.waocp.org/article_88737_bcae5dc35603dce875f30eca9050cc47.pdf
2019-09-01
2749
2755
10.31557/APJCP.2019.20.9.2749
breast cancer
Circulating microRNA
Biomarker
Neoadjuvant chemotherapy
Neemat M
Kassem
nkkassem@hotmail.com
1
Department of Clinical and Chemical Pathology, Kasr Al Ainy Centre of Clinical Oncology and Nuclear Medicine, School of Medicine, Cairo University, Cairo, Egypt.
AUTHOR
Wael S
Makar
wael_makar@yahoo.com
2
Department of Clinical Oncology, Kasr Al Ainy Centre of Clinical Oncology and Nuclear Medicine, School of Medicine, Cairo University, Cairo, Egypt.
AUTHOR
Hebatallah A
Kassem
heba.kasem@hotmail.com
3
Department of Clinical and Chemical Pathology, Kasr Al Ainy Centre of Clinical Oncology and Nuclear Medicine, School of Medicine, Cairo University, Cairo, Egypt.
LEAD_AUTHOR
Soha
Talima
soha_talima@hotmail.com
4
Department of Clinical Oncology, Kasr Al Ainy Centre of Clinical Oncology and Nuclear Medicine, School of Medicine, Cairo University, Cairo, Egypt.
AUTHOR
Mustafa
Tarek
mustafatareq.mt@gmail.com
5
Molecular oncology unit, Kasr Al-Ainy Centre of Clinical Oncology and Nuclear Medicine, School of Medicine, Cairo University, Cairo, Egypt.
AUTHOR
Hadeer
Hesham
hhaly_41@outlook.com
6
Faculaty of Science, Cairo University, Cairo, Egypt.
AUTHOR
Mohamed A
El-Desouky
madesouky@hotmail.com
7
Faculaty of Science, Cairo University, Cairo, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Molecular Screening of Nasopharyngeal Carcinoma: Detection of LMP-1, LMP-2 Gene Expression in Vietnamese Nasopharyngeal Swab Samples
Objective: This study aimed to characterize the expression of LMP-1, LMP-2 in clinical swab samples in orderto find out the potential molecular based biomarker for NPC diagnosis and screening, which could offer a chance indevelopment of rapid method for NPC diagnosis in Vietnamese population. Materials and Methods: A total of 93nasopharyngeal carcinoma swab samples and 100 healthy nasopharyngeal swab samples were collected to evaluateLMP-1, LMP-2 expression by Real-time reversed PCR. Results: we figured out the significant association betweenthe expression of LMP-1 (counting for 48.39%), LMP-2 (counting for 39.78%) and NPC. No LMP-1 expression wasobserved, and only 1 of 100 specimens was detected with LMP-2 positive in healthy samples. In the combination ofLMP-1 (+) and/or LMP-2 (+), the frequency of positive was 53.76%, greater than each gene expression. Additionally,sensitivity, specificity, positive predictive value, negative predictive value of assay were 99.00%, 98.04%, 69.72%,and 77.02%, respectively. Additionally, the LMP-2 expression level was 5.50 times higher in NPC samples thannon-cancerous samples. Conclusion: Our results indicated the molecular invasive method based on the expression ofLMP-1, LMP-2 in swab samples would be a promising supplement in NPC diagnosis, screening in the near future inVietnam.
https://journal.waocp.org/article_88712_534826f9c501375c64e3b0f72ae12f26.pdf
2019-09-01
2757
2761
10.31557/APJCP.2019.20.9.2757
LMP-1
LMP-2
Esptein-barr virus
nasopharngeal swab sample
Vietnam
Thuan Duc
Lao
thuan.ld@ou.edu.vn
1
Department of Pharmaceutical and Medical Biotechnology, Faculty of Biotechnology, Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam.
AUTHOR
Tuan Anh Hoang
Nguyen
nhatuan1710@gmail.com
2
Faculty of Biology and Biotechnology, University of Science, VNU-HCM, Vietnam.
AUTHOR
Kha Dong
Ngo
dongkha2607@gmail.com
3
Faculty of Biology and Biotechnology, University of Science, VNU-HCM, Vietnam.
AUTHOR
Hue Hong
Thieu
honghue0205@gmail.com
4
Faculty of Biology and Biotechnology, University of Science, VNU-HCM, Vietnam.
AUTHOR
Minh Trong
Nguyen
drnguyentrongminh@gmail.com
5
Cho Ray Hospital, HCMC, Vietnam.
AUTHOR
Dung Huu
Nguyen
drnguyenhuudung@gmail.com
6
Cho Ray Hospital, HCMC, Vietnam.
AUTHOR
Thuy Ai Huyen
Le
thuy.lha@ou.edu.vn
7
Department of Pharmaceutical and Medical Biotechnology, Faculty of Biotechnology, Ho Chi Minh City Open University, Ho Chi Minh City, Vietnam.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Signal Transduction of Improving Effects of Ibudilast on Methamphetamine Induced Cell Death
Objective: Interaction of methamphetamine and sigma (σ) receptors lead to up-regulation and activation of thesereceptors. The σ receptors induced apoptosis in some parts of the brain by increasing calcium, dopamine, ROS,mitochondrial pores and caspase activity. Ibudilast is a phosphodiesterase inhibitor and anti-inflammatory drug,which can decrease the inflammatory cytokines. Also, it has a neuroprotective effect. It seems that ibudilast canreduce the methamphetamine-induced cell death due to inhibition of σ receptors. Materials and Methods: Therewere seven treatments including; control: culture medium, Treatment 1: 1mM methamphetamine, Treatment 2: 1mMmethamphetamine and 1nM ibudilast, Treatment 3: 1mM methamphetamine and 10nM ibudilast, Treatment 4: 1mMmethamphetamine and 100nM ibudilast, Treatment 5: 1mM methamphetamine and 1uM ibudilast, Treatment 6: 1mMmethamphetamine and 10uM ibudilast, and Treatment 7: 1mM methamphetamine and 100uM ibudilast. Finally, forinhibition of PKA, CREB, IP3 receptor, NMDA receptor, Sigma receptor antagonist, sigma receptor agonist, cellswere preincubated with adding H89 dihydrochloride, 666-15, Heparin, Ketamine, BMY 14802, and Pentazocine.MTT and LDH tests were performed for cell viability and cytotoxicity measurement, respectively. In continuing, thecaspase activity colorimetric assay kit used for caspase 3 activity diagnosis. Rhodamine-123 performed to detection ofmitochondrial membrane potential. TUNEL test used to DNA fragmentation and apoptosis, Fura-2 used to Measurementof (Ca2+) ic and (Ca2+) m, and fluorescence microscope used to Measurement of antioxidant enzyme activities. Results:Ibudilast increased the cell viability and the rhodamine-123 absorbance in methamphetamin-treated PC12 cells. Itreduced cell cytotoxicity, caspase 3 activity, ic and m Ca2+ concentration, (OH) generation and DNA fragmentation inall concentrations of 1 nM t0 100 μM (p<0.05) by the optimal concentration of 100 μM, between our tested treatments.Conclusion: Ibudilast as a phosphodiesterase inhibitor can reduce the methamphetamine-induced cell death due toinhibition of σ receptors through cAMP production.
https://journal.waocp.org/article_88733_4db495f00f20c1e852a16e71c98555da.pdf
2019-09-01
2763
2774
10.31557/APJCP.2019.20.9.2763
Ibudilast
Methamphetamine
cell death
PC12
Reza
Tahvilian
rtahvilian@kums.ac.ir
1
Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.
AUTHOR
Komail
Amini
komail.amini@yahoo.com
2
Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran.
AUTHOR
Hossein
Zhaleh
hossain_jale@yahoo.com
3
Substance Abuse Prevention Research Center, Health Institute, Kermanshah University of Medical Sciences, kermanshah, Iran.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Clinical and Hematological Relevance of JAK2V617F, CALR, and MPL Mutations in Vietnamese Patients with Essential Thrombocythemia
Background: The picture of Vietnamese patients with essential thrombocythemia (ET) remains mostly undetermined.Our study intended to determine the frequency of JAK2V617F, CALR exon 9, and MPL exon 10 mutations as well asto analyze clinical characteristics associated with different mutational status in Vietnamese ET patients. Methods: Weexplored mutations of JAK2V617F, MPL, and CALR from 395 patients using allele specific oligonucleotide – polymerasechain reaction and Sanger sequencing techniques; then, the clinical and hematological features were compared accordingto mutation patterns. Results: We found that JAK2V617F, CALR exon 9, and MPL exon 10 mutations were present in56.2%, 27.6%, and 1% of the 395 patients with ET, respectively. Twelve different types of CALR mutation were detectedin 109 patients, with the CALR type 1 mutation (c.1099_1150del; L367fs*46) was the most common, followed byCALR type 2 mutation (c.1154_1155insTTGTC; K385fs*47). The JAK2V617F-positive patients had older age, higherwhite blood cell counts and higher hemoglobin levels but lower platelet counts than patients with CALR mutationsor patients negative for triple tests. There was no significant difference regarding sex ratio, white blood cell counts,platelet counts and hemoglobin levels among CALR mutation subtypes. Conclusion: we reported high frequency ofJAK2V617F, CALR, and MPL mutations in Vietnamese patients with ET and underscored the importance of combinedgenetic tests for diagnosis and classification of ET into different subtypes.
https://journal.waocp.org/article_88732_0a9b77309425f5913dc462a0eff56b58.pdf
2019-09-01
2775
2780
10.31557/APJCP.2019.20.9.2775
essential thrombocythemia
JAK2V617F
CALR
MPL
Vietnam
Hoang Anh
Vu
hoanganhvu@ump.edu.vn
1
Center for Molecular Biomedicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.
AUTHOR
Tran Thi
Thao
thienduong.thao@gmail.com
2
Department of Hematology, Faculty of Medicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.
AUTHOR
Cao Van
Dong
bio_hailam@yahoo.com
3
Ho Chi Minh City Blood Transfusion and Hematology Hospital, Ho Chi Minh City, Vietnam.
AUTHOR
Nguyen Lam
Vuong
nguyenlamvuong@gmail.com
4
Department of Medical Statistics and Informatics, Faculty of Public Health, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.
AUTHOR
Ho Quoc
Chuong
quocchuong0712@gmail.com
5
Center for Molecular Biomedicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.
AUTHOR
Phan Nguyen Thanh
Van
vanntp@yahoo.com
6
Ho Chi Minh City Blood Transfusion and Hematology Hospital, Ho Chi Minh City, Vietnam.
AUTHOR
Huynh
Nghia
nghiahoathuphuong@gmail.com
7
Department of Hematology, Faculty of Medicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.
AUTHOR
Nguyen Tan
Binh
nguyentanbinhmd@gmail.com
8
Ho Chi Minh City Blood Transfusion and Hematology Hospital, Ho Chi Minh City, Vietnam.
AUTHOR
Phu Chi
Dung
chidungyk85@yahoo.com
9
Ho Chi Minh City Blood Transfusion and Hematology Hospital, Ho Chi Minh City, Vietnam.
AUTHOR
Phan Thi
Xinh
phanthixinh@yahoo.com
10
Department of Hematology, Faculty of Medicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Targeting Epidermal Growth Factor Receptor by MiRNA-145 Inhibits Cell Growth and Sensitizes NSCLC Cells to Erlotinib
Background: Despite effective activity of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs), such as erlotinib, all non-small cell lung cancer (NSCLC) patients eventually acquire resistance to these agents. Studies have demonstrated that down-regulation of miRNA-145 leads to enhancement of EGFR expression, cell proliferation and metastasis. The aim of this study was to investigate the effect of miRNA-145 on sensitivity of the A549 NSCLC cells to erlotinib. Methods: Quantitative real-time PCR was used to examine the effect of miRNA-145 on EGFR expression. The effect of miRNA-145 on cell growth and sensitivity the lung cancer cells to erlotinib was examined by trypan blue and MTT assays, respectively. The combination index was calculated using the non-constant method of Chou-Talalay. Apoptosis was determined by ELISA cell death assay. Results: We found that miRNA-145 was markedly suppressed the expression of EGFR and inhibited the cancer cell growth, relative to blank control and negative control miRNA (p<0.05). Pretreatment with miRNA-145 synergistically enhanced the sensitivity of the lung cancer cells to erlotinib. Results of apoptosis assay revealed that miRNA-145 can induce apoptosis and increase the erlotinib-mediated apoptosis. Conclusions: Our data demonstrate that miRNA-145 play a critical role in the lung cancer cell growth, survival and EGFR-TKIs resistance possibly by regulation of EGFR. Therefore, miRNA-145 replacement therapy can become a new therapeutic strategy in lung cancer.
https://journal.waocp.org/article_88725_341d14d46efadfd9777ab2e343d00924.pdf
2019-09-01
2781
2787
10.31557/APJCP.2019.20.9.2781
Apoptosis
growth
Lung cancer
MiRNA-145
tyrosine kinase inhibitor
Jamal
Amri
jamal.amri71@gmail.com
1
Molecular and Medicine Research Center, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
AUTHOR
Neda
Molaee
n.molee91@gmail.com
2
Department of Molecular Medicine and Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
AUTHOR
Maryam
Baazm
dr.baazm@arakmu.ac.ir
3
Department of Anatomy, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
AUTHOR
Hadi
Karami
h.karami@arakmu.ac.ir
4
Molecular and Medicine Research Center, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
LEAD_AUTHOR
ORIGINAL_ARTICLE
North Korea Must be Global Scale Cohort, Not a Galapagos in the Medical Research Field
Objective: This study aimed to compare the research trend regarding gynecologic malignancies in North Korean medical journal and South Korean medical journal. Methods: Articles published in the journal of “Pediatrics, Obstetrics, and Gynecology” in North Korea and “Obstetrics & Gynecology Science” in South Korea from 2006 to 2016 were analyzed by using frequency analysis. Studies on gynecologic malignancies were classified by international classification of disease (ICD-10). Results: Out of 3361 reviewed articles, 116 articles published in North Korean journal and 519 articles published in South Korean medical journal were classified as gynecologic oncology. We found a distinct difference between North and South Korean medical journals regarding research trends on gynecologic oncology. The proportions of gestational trophoblastic disease, cervical cancer, and anogenital warts were higher in North Korean medical journal, but proportions of ovarian cancer, fallopian tube cancer, peritoneal cancer, corpus uterine cancer, and vulvar cancer were higher in South Korean medical journal. Conclusion: This study enforced an analysis of research trends on gynecologic malignancies in North Korean and South Korea medical journals, and a distinct difference was observed in this regard. In the future, grand scale cohort study in the genetic identical two Korean population is needed for research of environmental effect on gynecologic cancer.
https://journal.waocp.org/article_88722_2a7176c930caa4af99d90d5c475c9a08.pdf
2019-09-01
2789
2794
10.31557/APJCP.2019.20.9.2789
research trends
gynecologic cancer
North Korea
socioeconomic disparity
genetic identity
Duck Yeong
Ro
duckyeong20@naver.com
1
Department of Obstetrics and Gynecology, Incheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
AUTHOR
Dae Hee
Kim
md.kim.daehee@gmail.com
2
Department of Emergency Medicine, Incheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
AUTHOR
Sae Hyun
Park
park7609@gmail.com
3
Department of Obstetrics and Gynecology, Incheon-sarang Hospital, Incheon, Republic of Korea.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Effectiveness of a Self-Monitoring Quality of Life Intervention For Patients with Cancer Receiving Palliative Care: A Randomized Controlled Clinical Trial
Background: Use of patient-reported outcome measures in routine clinical practice has important benefits forpatients with cancer. To examine the effect of a self-monitoring quality of life (QOL) intervention on global QOL andphysical and emotional function in patients with cancer receiving palliative care. Methods: Prospective randomizedstudy had been undertaken at Toshima Hospital, Japan. This study compared an intervention group that completed theshortened Care Notebook booklet versus a control group that received usual care. The primary outcome was global QOLand secondary outcomes were physical and emotional function. Participants completed the European Organization forResearch and Treatment of Cancer Quality of Life Questionnaire Core 15 Palliative at baseline, and at 1 and 3 weeks.The effects of the intervention were evaluated with a linear mixed-effects model. Results: Forty-three patients wererandomized. One patient in each group could not receive the allocated intervention, leaving 41 patients for inclusionin the modified intention-to-treat (ITT) analysis for the primary outcome. Twenty-seven patients were analyzed for thesecondary outcomes using per protocol set (PPS). The ITT analysis showed no significant overall effect on global QOL(P=0.285), but the PPS analysis showed a significant overall effect on global QOL (P=0.034) and physical function(P=0.047) for group difference over time in the linear mixed-effects model. Conclusions: Use of the Care Notebookmight have beneficial effects. The results could be interpreted as the effectiveness of the intervention of the CareNotebook for with cancer receiving palliative care.
https://journal.waocp.org/article_88726_e7218815761240f83fac8c20ddaee6f0.pdf
2019-09-01
2795
2802
10.31557/APJCP.2019.20.9.2795
cancer
Quality of Life
palliative care
self-monitoring
Ayako
Matsuda
amatsuda@med.teikyo-u.ac.jp
1
Teikyo University School of Medicine, Department of Hygiene and Public Health, Tokyo, Japan.
LEAD_AUTHOR
Yosuke
Yamada
yousuke_yamada@tokyo-hmt.jp
2
Toshima Hospital, Division Chief of Palliative Care Unit, Tokyo, Japan.
AUTHOR
Noriko
Ishizuka
blue.hippopo@gmail.com
3
Tokyo Medical and Dental University, Graduate School of Medical and Dental Sciences, Section of Liaison Psychiatry and Palliative Medicine, Tokyo, Japan.
AUTHOR
Eisuke
Matsushima
matsuda@med.teikyo-u.ac.jp
4
Tokyo Medical and Dental University, Graduate School of Medical and Dental Sciences, Section of Liaison Psychiatry and Palliative Medicine, Tokyo, Japan.
AUTHOR
Kunihiko
Kobayashi
kobakuni@saitama-med.ac.jp
5
Saitama Medical University International Medical Center, Department of General Thoracic Surgery, Saitama, Japan.
AUTHOR
Takayoshi
Ohkubo
tohkubo@med.teikyo-u.ac.jp
6
Teikyo University School of Medicine, Department of Hygiene and Public Health, Tokyo, Japan.
AUTHOR
Kazue
Yamaoka
kazue@med.teikyo-u.ac.jp
7
Teikyo University Graduate School of Public Health, Tokyo, Japan.
AUTHOR
ORIGINAL_ARTICLE
The Psychometric Properties of the Center for Epidemiological Studies Depression Scale (CES-D) for Iranian Cancer Patients
Objectives: The Center for Epidemiologic Studies Depression Scale (CES-D) was specifically created to assessdepression in cancer patients. However, to date, the CES-D has not been validated in Farsi. Therefore, this study aimed toassess the psychometric properties of the CES-D in Iranian cancer patients. Methods: During a three-month period(October to December, 2015), a total of 380 cancer patients completed a Farsi version of the CES-D. The constructvalidity of the scale was evaluated by exploratory factor analysis. Reliability was assessed using Cronbach’s alphaand McDonald Omega. All of the statistical procedure were run by SPSS 22 (SPSS Inc., Chicago, IL, USA). Results:The construct validity of the CES-D determined three factors (somatic affect, negative affect, and positive affect),which explained 65.60% of the total variance. The internal consistency was greater than 0.70. Conclusion: Findingsrevealed that the Farsi version of the CES-D has acceptable validity and reliability, which can be used to measuredepression in Iranian cancer patients.
https://journal.waocp.org/article_88731_4f14fe0d2e7a557dffe7d066c5aad036.pdf
2019-09-01
2803
2809
10.31557/APJCP.2019.20.9.2803
cancer
depression
epidemiological
Iran
Psychometrics
Hamid
Sharif Nia
pegadis@yahoo.com
1
School of Nursing and Midwifery Amol, Mazandaran University of Medical Sciences, Sari, Iran.
AUTHOR
Maryam
Rezapour
mr.mns.mr@gmail.com
2
Psychiatry and Behavioral Sciences Research Center, Addiction Institute, Department of Psychiatry, Mazandaran University of Medical Sciences, Sari, Iran.
AUTHOR
Kelly A
Allen
drkellyallen@gmail.com
3
The Melbourne Graduate School of Education, the University of Melbourne, Melbourne, Australia.
AUTHOR
Saeed
Pahlevan Sharif
samsharif6@gmail.com
4
Taylor’s Business School, Taylor’s University Malaysia, Subang Jaya, Malaysia.
AUTHOR
Azar
Jafari
jafaria241@gmail.com
5
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
AUTHOR
Hojjat
Torkmandi
hojjat.or@gmail.com
6
Operating Room Group, Department of Nursing, School of Nursing and Midwifery, Zanjan University of Medical Sciences, Zanjan, Iran.
AUTHOR
Amir Hossein
Goudarzian
amir_sari@yahoo.com
7
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Mammographic Breast Density of Japanese Women Living in Australia: Implications for Breast Screening Policy
Background: Mammographic Breast Density (MBD) increases breast cancer risk, lowers sensitivity of mammographyand is related to ethnicity. This study compared the MBD of Australian women living in Australia (AW), Japanesewomen living in Japan (JWJ) and Japan-born women living in Australia (JWA). The outcomes have implications forbreast screening policies in Australia. Methods: In this cross-sectional study, mammographic images were collectedfrom a total of 677 women who were examined at BreastScreen NSW or Miyata Hospital, Japan. The images wereretrospectively evaluated using the 5th edition Breast Imaging-Reporting and Data System (BI-RADS) to assessthe level of MBD. Descriptive analyses and binary logistic regressions were performed. Results: More than 65% ofAW had low MBD while the majority of JWJ had high MBD. Among AW, low MBD was found in women aged 40-49and 50-59. The MBD of JWA showed a similar pattern to JWJ but with a slightly lower MBD. The great majority ofJWA aged 40-49 were found to have high MBD compared to women aged 50-59. JWJ were categorised as high MBDregardless of their age. JWA were 5 times more likely and JWJ were 15 times more likely to have high MBD compared toAW. Conclusion: Mammography screening becomes more effective for JW after migration to Australia. For JWAaged 40-49 years, supplemental screening options are indicated to provide better cancer detection. For AW, screeningmammography could be equally as effective for AW aged 40-49 years as for those aged 50-59.
https://journal.waocp.org/article_88736_faa3ebe3ec7fc15185ab65b9577a1b94.pdf
2019-09-01
2811
2817
10.31557/APJCP.2019.20.9.2811
Breast density
mammography
Japan
Australia
Early Detection of Cancer
Miwa M
Mizukoshi
mmiz2147@uni.sydney.edu.au
1
Discipline of Behavioural and Social Sciences in Health, The University of Sydney, Australia.
LEAD_AUTHOR
Syeda Z
Hossain
zakia.hossain@sydney.edu.au
2
Discipline of Behavioural and Social Sciences in Health, The University of Sydney, Australia.
AUTHOR
Ann
Poulos
ann.poulos@sydney.edu.au
3
Discipline of Medical Radiation Sciences, The University of Sydney, Australia.
AUTHOR
ORIGINAL_ARTICLE
Determination of Cut Point in the Age of Colorectal Cancer Diagnosis Using a Survival Cure Model
Background and Objectives: Colorectal Cancer (CRC) is the fourth cancer-related cause of death worldwide.CRC is a multi-stage cancer, which is curable during the early stages of the disease. Therefore, determining the time ofcut-point existence could improve treatment planning and help directly allocate resources. This study aims to determinethe cut point in the age of CRC diagnosis. Methods: This study, covering the course 1985 to 2012, consisted of 345colorectal cancer patients registered in Taleghani Hospital, Tehran, Iran and followed up to 2013. The cut-point in theage of CRC diagnosis was obtained using a mixture cure model. The data were analyzed using SPSS and R, V. 20 and2.15.0, respectively. Results: The results showed that the cut point in the age of CRC diagnosis was 50 years. Basedon our estimation, 65% of the patients diagnosed with CRC at or younger than 50 were cured, while 31% of themdiagnosed older than 50 were cured, and the younger group had a better survival over the older group. Conclusion:Since access to a cut-point and analysis of created prognostic groups are important in screening and treatment planning,our results suggested that it is better to estimate the cut-point in the age of curable cancers in early stages via survivalcure models, and the cure rate would increase by CRC timely screening.
https://journal.waocp.org/article_88715_f451e30f9eda53356c2300df9e0371cc.pdf
2019-09-01
2819
2823
10.31557/APJCP.2019.20.9.2819
colorectal cancer
Survival Analysis
survival cure model
change point
cut point
Mahbobe
Abdollahi
mahbobe.abdollahi@modares.ac.ir
1
Department of Public Health, School of Health, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran.
LEAD_AUTHOR
Nayereh
Kasiri
kasirin1@thums.ac.ir
2
Department of Public Health, School of Health, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran.
AUTHOR
Mohamad Amin
Pourhoseingholi
amin_phg@yahoo.com
3
Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Ahmad Reza
Baghestani
mbaoth2@yahoo.com
4
Department of Biostatistics, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Habibollah
Esmaily
mbaoth1@yahoo.com
5
Department of Biostatistics, Faculty of Health, Mashhad University of Medical Sciences, Mashhad, Iran.
AUTHOR
ORIGINAL_ARTICLE
The Association of Dietary Intake of Calcium and Vitamin D to Colorectal Cancer Risk among Iranian Population
Background: Vitamin D and Calcium have a possible protective impact versus rectal neoplasm. Vitamin D, animportant nutrient, is vital to regulate the absorption of calcium and bone mineralization; nevertheless, in a case-controlstudy in Iran, we investigated the relationship among the dietary intake of vitamin D and calcium with the hazard ofrectal neoplasm. Methods: 363 subjects (162 cases and 201 controls) participated in the case- control Study fromMarch 2017 to November 2018. Dietary intake of Calcium and Vitamin D was calculated using a 148-items foodfrequencyquestionnaire. Results: Since altering the strong confounding agents, the multivariate risk proportion withinthe dietary vitamin D intake was OR=0.2, 95%CI 0.1-0.5, P_value case of calcium and rectal cancer. Conclusions: Taken together, a possible reduction in the hazard of rectal neoplasmwith dietary intake of Vitamin D within Iranian patients was observed.
https://journal.waocp.org/article_88746_61bfe8bb7199f8ce661feeac3c7646d1.pdf
2019-09-01
2825
2830
10.31557/APJCP.2019.20.9.2825
cancer
Vitamin D
Calcium
Rectal
Payam
Hosseinzadeh
payamhosseinzadehh@yahoo.com
1
Gastrointestinal and Liver Diseases Research Center (GILDRC), Iran University of Medical Sciences (IUMS), Tehran, Iran.
AUTHOR
Mohammad
Javanbakht
armanjvbt81@gmail.com
2
Nephrology and Urology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
AUTHOR
Mahdi
Alemrajabi
mahdialemrajabii@yahoo.com
3
Firoozgar Hospital, FCRDC, Iran University of Medical Sciences, Tehran, Iran.
AUTHOR
Ali
Gholami
aligholamii@gmail.com
4
Noncommunicable Diseases Research Center, Neyshabur University of Medical Sciences, Neyshabur, Iran.
AUTHOR
Bahare
Amirkalali
bahareamirkalalii@gmail.com
5
Gastrointestinal and Liver Diseases Research Center (GILDRC), Iran University of Medical Sciences (IUMS), Tehran, Iran.
AUTHOR
Masoudreza
Sohrabi
masoudrezassohrabi@gmail.com
6
Gastrointestinal and Liver Diseases Research Center (GILDRC), Iran University of Medical Sciences (IUMS), Tehran, Iran.
AUTHOR
Farhad
Zamani
zamani.farhad@gmail.com
7
Gastrointestinal and Liver Diseases Research Center (GILDRC), Iran University of Medical Sciences (IUMS), Tehran, Iran.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Cytotoxicity Profiling of Annona Squamosa in Cancer Cell Lines
Objective: In the study our aim was to evaluate the cytotoxic activity of different solvent extracts of Annona squamosa seeds. Methods and materials: The four extracts used were petroleum ether, chloroform, ethyl acetate and methanol were tested using cytotoxicity assays. Results: Among the four extracts tested petroleum ether showed maximum cytotoxicity against a panel of cancer cell lines such as nasopharyngeal cancer (KB) cells, lung cancer (A-549) cells, breast cancer (MCF- 7) cells, leukemic (K-562) cells and inhibited the growth of murine cancer cells such as Dalton’s lymphoma ascites (DLA) and Ehrlich ascites carcinoma (EAC). Conclusion: Petroleum ether extract of Annona squamosa seeds showed cytotoxicity towards a panel of cancer cells meanwhile non-significant cytotoxicity towards normal cells.
https://journal.waocp.org/article_88723_9b8fff9d568a72f1a6f5f27db45b4e5d.pdf
2019-09-01
2831
2840
10.31557/APJCP.2019.20.9.2831
Anticancer
Cytotoxicity
Annona squamosa
Biba
Vikas
bibavikas@gmail.com
1
Regional Cancer Centre, Thiruvananthapuram, India.
AUTHOR
Sukumaran
Anil
dranils@gmail.com
2
Department of Dentistry, Hamad Medical Corporation, Doha, Qatar.
LEAD_AUTHOR
P
Remani
drremanipr@gmail.com
3
Regional Cancer Centre, Thiruvananthapuram, India.
AUTHOR
ORIGINAL_ARTICLE
PIK3CA and PTEN Genes Expressions in Breast Cancer
Background: The phosphatidylinositol-3 kinase (PI3K) intracellular signaling pathway plays an importantrole in breast cancer. The current study aimed to evaluate the expressions of two main regulators of PI3K pathway;phosphatidylinositol-3- kinase catalytic subunit alpha as activator (PIK3CA), and phosphatase and tensin-homologas inhibitor (PTEN), in breast carcinoma tissue, and compare with their expressions in adjacent normal breast tissue.Methods: A total of fifty female patients with breast carcinoma from surgical oncology unit of Alexandria-MainUniversity Hospital were included in this study. The Quantitative Real Time PCR was used to quantify expressions ofPIK3CA and PTEN. Results: PIK3CA mRNA expression was significantly increased in breast cancer tissues comparedto normal breast tissues (P<0.001, Z=5.700), also PTEN mRNA expression was significantly higher in breast carcinomatissue compared to normal breast tissue (P<0.001, Z=5.362). Conclusion: Increased the expressions of PIK3CA andPTEN mRNA in breast cancer tissue compared to normal breast tissue.
https://journal.waocp.org/article_88745_424f3bb723f6b177971f007707b3b285.pdf
2019-09-01
2841
2846
10.31557/APJCP.2019.20.9.2841
PIK3CA
PTEN
Expressions
breast cancer
Nabil Hadi
Alowiri
nabilhadii14@gmail.com
1
Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.
LEAD_AUTHOR
Shaden Muawia
Hanafy
shadenmuawia@yahoo.com
2
Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.
AUTHOR
Reham Abdel
Haleem
rehamhalem@hotmail.com
3
Department of Clinical Pathology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
AUTHOR
Ahmed
Abdellatif
maeennabil2005@gmail.com
4
Surgical Oncology Unit, Department of General Surgery, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Association of Abnormal Pap Smear with Occult Cervical Stromal Invasion in Patients with Endometrial Cancer
Objective: The purpose of this study was to determine the association between abnormal preoperative Pap smearresults and occult cervical stromal invasion in endometrial cancer patients. Methods: Medical records were reviewed ofpatients with endometrial cancer who had undergone surgical staging at Srinagarind Hospital. Patients with gross cervicalinvolvement, with an unsatisfactory Pap smear, without available Pap smear results, with no cervical intraepitheliallesion/invasive cervical cancer, or who had previously undergone pelvic radiation therapy were excluded. The patientswere assigned to one of two groups according their Pap smear results (negative and epithelial cell abnormalities).Logistic regression was used to determine the independent association between an abnormal Pap smear and the riskof cervical stromal invasion. Results: All cervical smears in this study were performed as conventional Pap smears.Smears were abnormal in 50 (21.0%) of the 238 patients enrolled and normal in the remaining 188 (79.0%). The typesof Pap smear abnormalities included adenocarcinoma (n=22); atypical endometrial cells (n=2); atypical glandular cells(n=17); high-grade squamous intraepithelial lesions (n=4); atypical squamous cells, cannot exclude high-grade squamousintraepithelial lesions (n=2); and atypical squamous cells of undetermined significance (n=3). After controlling for type ofendometrial cancer, abnormal Pap smear results were found to be a significant independent factor that indicated cervicalstromal invasion (adjusted OR 2.65; 95% CI 1.35 to 5.21). Conclusion: Endometrial cancer patients with abnormalPap smears were strongly and independently associated with histopathologically diagnosed cervical stromal invasion.
https://journal.waocp.org/article_88750_868ce01109854b3cf90522890e4fdd62.pdf
2019-09-01
2847
2850
10.31557/APJCP.2019.20.9.2847
Pap smear
Endometrial cancer
cervical stromal invasion
risk factors
Kewalin
Khumthong
kewalin.eye35@gmail.com
1
Department of Obstetrics and Gynaecology, Faculty of Medicine, Khon Kaen University, Khon Kean, Thailand.
AUTHOR
Apiwat
Aue-Aungkul
apiwat_ant@hotmail.com
2
Department of Obstetrics and Gynaecology, Faculty of Medicine, Khon Kaen University, Khon Kean, Thailand.
LEAD_AUTHOR
Pilaiwan
Kleebkaow
kpilai@kku.ac.th
3
Department of Obstetrics and Gynaecology, Faculty of Medicine, Khon Kaen University, Khon Kean, Thailand.
AUTHOR
Bandit
Chumworathayi
bunchu@kku.ac.th
4
Department of Obstetrics and Gynaecology, Faculty of Medicine, Khon Kaen University, Khon Kean, Thailand.
AUTHOR
Amornrat
Temtanakitpaisan
kunglekmed@hotmail.com
5
Department of Obstetrics and Gynaecology, Faculty of Medicine, Khon Kaen University, Khon Kean, Thailand.
AUTHOR
Wilasinee
Nhokaew
pattrusia@gmail.com
6
Department of Obstetrics and Gynaecology, Faculty of Medicine, Khon Kaen University, Khon Kean, Thailand.
AUTHOR
ORIGINAL_ARTICLE
Evaluation of Patched-1 Protein Expression Level in Low Risk and High Risk Basal Cell Carcinoma Subtypes
Objective: Basal cell carcinoma (BCC) is the most common malignancy in humans and represents a growingpublic health care problem. The major etiological factors contributing to BCC development are exposure to ultravioletradiation and genetic alterations. BCC is primarily caused by dysregulation of sonic Hedgehog (HH) signaling pathwayin basal cells of the skin. BCC can be classified into low risk non-aggressive and high risk aggressive subtypes. BCCsubtypes differentiation is essential for prognosis and for better disease management and treatment strategies. Theaim of this study was to assess the correlation between PTCH1 protein expression level and the aggressiveness ofBCC histopathology. Methods: Archival paraffin embedded blocks containing BCC were retrieved from a cohort of101 patients. Immunohistochemistry staining was performed to assess the expression level of PTCH1 which is a keycomponent of Hedgehog pathway. Results: 101 paraffin embedded samples were evaluated and classified as high riskand low risk BCC subtypes by histopathological finding. High risk BCC subtypes were found in 40 samples (39.6%)and low risk subtypes were identified in 61 samples (60.4%). Nodular was the most frequent subtype which was foundin (56/ 101), followed by infiltrative (22/101) and micronodular (14/ 101) subtypes. Positive PTCH1 expression wasfound highest in nodular subtypes (46.5%). Conclusion: In this study, the correlation between low risk or high riskBCC subtypes and PTCH1 expression level was not statistically significant (p>0.05), but the frequency of positivePTCH1 expression was found to be higher in low risk subtypes than high risk BCC subtypes.
https://journal.waocp.org/article_88755_89d4a578424711b89808dc1ef9515796.pdf
2019-09-01
2851
2857
10.31557/APJCP.2019.20.9.2851
Basal cell carcinoma
PTCH1
high risk BCC
low risk BCC
Rowida
Almomani
rfalmomani7@just.edu.jo
1
Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan.
LEAD_AUTHOR
Mariam
Khanfar
mariamkhanfar94@gmail.com
2
Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan.
AUTHOR
Khaldon
Bodoor
khaldon_bodoor@just.edu.jo
3
Department of Applied Biology, Jordan University of Science and Technology, Irbid, Jordan.
AUTHOR
Firas
Al-Qarqaz
faalqarqaz@yahoo.com
4
Department of Dermatology, Jordan University of Science and Technology, Irbid, Jordan.
AUTHOR
Mohammad
Alqudah
m.alqudah12@just.edu.jo
5
Department of Pathology, Jordan University of Science and Technology, Irbid, Jordan.
AUTHOR
Hanan
Hammouri
hmhammouri@just.edu.jo
6
Department of Mathematics and Statistics, Faculty of Science and Arts, Jordan University of Science and Technology, Irbid, Jordan.
AUTHOR
Asma
Abu-Salah
asma_abusalah@hotmail.com
7
Department of Pathology, Jordan University of Science and Technology, Irbid, Jordan.
AUTHOR
Yazan
Haddad
yazanhaddad@hotmail.com
8
Department of Applied Biology, Jordan University of Science and Technology, Irbid, Jordan.
AUTHOR
Wisam
Al Gargaz
wagargaz@just.edu.jo
9
Orthopedic Surgery Division, Special Surgery Department, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.
AUTHOR
Ziyad
Mohaidat
zmmohaidat@just.edu.jo
10
Orthopedic Surgery Division, Special Surgery Department, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan.
AUTHOR
ORIGINAL_ARTICLE
High Effective of 14-Day High-Dose PPI- Bismuth-Containing Quadruple Therapy with Probiotics Supplement for Helicobacter Pylori Eradication: A Double Blinded-Randomized Placebo-Controlled Study
Background: Helicobacter pylori (H. pylori) infection is important risk factors for chronic gastritis, peptic ulcer andgastric cancer. Bismuth-containing quadruple therapy has recently been the first-line regimen recommended in manyEuropean countries but has limited efficacy in ASEAN especially Thailand. This study was aim to evaluate efficacy ofhigh dose PPI Bismuth-containing quadruple therapy with probiotics supplement for H. pylori eradication. Methods:In this double-blind randomized placebo-controlled study, H. pylori infected patients were randomized to receive 7-or14-day high dose PPI- bismuth-containing quadruple therapy with or without probiotics supplement. Probiotic was37.5 mg Lactobacillus reuteri (Biogaia®) in tablet twice daily. CYP2C19 genotyping and antibiotic susceptibility testswere also done. H. pylori eradication was defined as a negative 13C-urea breath test at least 4 weeks after treatment.Results: 100 subjects were enrolled (72 females, 28 males, mean age=54 years). Antibiotic resistance was 15.6% forclarithromycin, 34.1% for metronidazole. CYP2C19 genotyping was performed in both group and revealed 13%, 50% and37% for poor, intermediate and rapid metabolizers, respectively. Overall eradication rates of 7-day and 14-day regimenswith probiotic were 68% and 96%; P value=0.027. The eradication rate for all patients with poor and rapid metabolizerswere 100% with 14-day regimen. 14-day regimen with probiotics can provide 100% eradication with clarithromycinresistance, metronidazole resistance or dual clarithromycin and metronidazole resistance group. Furthermore, theincidence of nausea and vomiting, abdominal discomfort, and bitter taste were significantly lower in patients withprobiotics group compared with placebo (6%vs.26%, P=0.002,OR=0.126,95% CI=0.03-0.53; 4%vs.18.0%, P=0.017,OR= 0.155,95% CI=0.03-0.81 and 4%vs.26%, P= 0.001,OR= 0.08, 95%CI= 0.016-0.41, respectively). Conclusions:The 14-day high dose PPI- bismuth-containing quadruple therapy with probiotic can provide an excellent cure rate forH. pylori infection as first line treatment irrespective of CYP2C19 and antibiotic resistance pattern. Adding probioticalso significantly reduced treatment-related adverse events and improve the patients’ compliance.
https://journal.waocp.org/article_88743_6264df018ebedbb8d4e095dc77dd0b44.pdf
2019-09-01
2859
2864
10.31557/APJCP.2019.20.9.2859
High-dose PPI
quadruple therapy
probiotics supplement
Helicobacter pylori
Piyakorn
Poonyam
piyakorn@g.swu.ac.th
1
Gastroenterology Unit, Thammasat University Hospital, Pathumthani, Thailand.
AUTHOR
Peranart
Chotivitayatarakorn
peimpulse@gmail.com
2
Gastroenterology Unit, Thammasat University Hospital, Pathumthani, Thailand.
AUTHOR
Ratha-Korn
Vilaichone
vilaichone@hotmail.co.th
3
Gastroenterology Unit, Thammasat University Hospital, Pathumthani, Thailand.
LEAD_AUTHOR
ORIGINAL_ARTICLE
Validity and Reliability of Health Belief Model Questionnaire for Promoting Breast Self-examination and Screening Mammogram for Early Cancer Detection
Background: Early detection of breast cancer is essential in improving overall women’s health. The researchers sought to develop a comprehensive measure that combined the basic components of the health belief model (HBM) with a focus on breast self-examination (BSE) and screening mammogram amongst women.Methods: Questionnaire items were developed following a review of relevant literature of HBM on BSE and screening mammogram. The sampling frame for the study was Malaysian women aged 35 to 70 years old, living in Kuantan, Pahang and able to read or write in Bahasa Malaysia or English. As such, 103 women were randomly selected to participate in the study. Tests of validity using exploratory factor analysis (EFA) and reliability were subsequently performed to determine the psychometric properties of the questionnaire. Results: The EFA revealed nine factors (self-efficacy of mammogram, perceived barriers of BSE and mammogram, perceived susceptibility of breast cancer, perceived severity of breast cancer, cues to action for mammogram screening, perceived benefits of BSE, health motivation, perceived benefits of mammogram and self-efficacy of BSE) containing 54 items that jointly accounted for 74.2% of the observed variance. All nine factors have good internal consistency with Cronbach’s alpha ≥ 0.8. Fifty-four items remained in the final questionnaire after deleting 13 problematic items. The scale also showed good convergent and discriminant validity.Conclusion: The findings showed that the designed questionnaire was a valid and reliable instrument for the study involving women in Kuantan, Pahang. The instrument can help to assess women’s beliefs on BSE adoption and mammogram screening in health care practice and research.
https://journal.waocp.org/article_88749_6ea64228e24477de8e268e97c93d7d4c.pdf
2019-09-01
2865
2873
10.31557/APJCP.2019.20.9.2865
Health Belief Model
Validation
Reliability
Breast self-examination
Mammogram
Norfariha
Che Mohamed
fareehafarry@gmail.com
1
Department of Diagnostic Imaging and Radiotherapy, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia (IIUM), Kuantan Campus, Pahang, Malaysia.
AUTHOR
Soo-Foon
Moey
moeysf@iium.edu.my
2
Department of Diagnostic Imaging and Radiotherapy, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia (IIUM), Kuantan Campus, Pahang, Malaysia.
LEAD_AUTHOR
Bee Chiu
Lim
bclimcrc@gmail.com
3
Clinical Research Centre, Hospital Tengku Ampuan Afzan (HTAA), Kuantan, Pahang, Malaysia.
AUTHOR
ORIGINAL_ARTICLE
Thyroid Cancer in Kazakhstan: Component Analysis of Incidence Dynamics
Background: The International Agency for Research on Cancer (IARC) reports that 567,000 new cases of thyroidcancer (TC) were registered in the world in 2018, and the age-standardized incidence rate was 6.7 per 100,000. TheGlobal Cancer Observation forecasts a 35% growth in the number of new cases worldwide by 2040. The number ofpatients with TC in Kazakhstan is also increasing steadily. This investigation was the first epidemiological study of TCtrends by component analysis among the population of Kazakhstan. This paper presents the results of the componentanalysis of TC incidence trends in Kazakhstan. Methods: The study covers primary data of TC cases (ICD 10 – C73)registered throughout Kazakhstan from 2009 to 2018. TC incidence trends were evaluated using component analysisaccording to the methodological recommendations. Results: 5,559 new TC cases were registered during the 10-yearstudy period. The average age of patients was 52.0±0.2 years, the average annual age-standardized rate in 2009-2018was 3.3±0.20/0000, with a constant upward trend (Т=+6.6%). According to the component analysis results, the increasein incidence was mainly due to the combined effect of the two factors: the increased disease risk (ΔR=+61.7%), andthe population growth (ΔP=+15.4%). Conclusion: The noted increase in incidence was mainly caused by the changesin risk factors, such as the worsening environmental aspects and the increase in detection of clinically non-manifestingcases. The results of the study shall be taken into account when planning anticancer activities for TC.
https://journal.waocp.org/article_88759_2edcb241dc12fb7b204b08fc0b49637d.pdf
2019-09-01
2875
2880
10.31557/APJCP.2019.20.9.2875
Thyroid cancer
Incidence
Component analysis
Kazakhstan
Nurbek
Igissinov
n.igissinov@gmail.com
1
Astana Medical University, Nur-Sultan, Kazakhstan.
LEAD_AUTHOR
Saken
Kozhakhmetov
s.k.kozhakhmetov@gmail.com
2
Astana Medical University, Nur-Sultan, Kazakhstan.
AUTHOR
Marzhan
Zhantubetova
dr.zhantubetova@gmail.com
3
Astana Medical University, Nur-Sultan, Kazakhstan.
AUTHOR
Gulnur
Igissinova
gulnurs@mail.ru
4
Central Asian Cancer Institute, Nur-Sultan, Kazakhstan.
AUTHOR
Zarina
Bilyalova
z.bilyalova@gmail.com
5
Central Asian Cancer Institute, Nur-Sultan, Kazakhstan.
AUTHOR
Gulnur
Akpolatova
akpolatova@mail.ru
6
Astana Medical University, Nur-Sultan, Kazakhstan.
AUTHOR
Vladimir
Lyust
vladimir.lyust@gmail.com
7
Astana Medical University, Nur-Sultan, Kazakhstan.
AUTHOR
Sarsembi
Koblandin
koblandin.s@mail.ru
8
Astana Medical University, Nur-Sultan, Kazakhstan.
AUTHOR
Dulat
Turebayev
tdk_1410@mail.ru
9
Astana Medical University, Nur-Sultan, Kazakhstan.
AUTHOR
Kairat
Adaibayev
kajratadajbaev@gmail.com
10
Astana Medical University, Nur-Sultan, Kazakhstan.
AUTHOR
Ardak
Omarbekov
omarbekovardak1975@gmail.com
11
Astana Medical University, Nur-Sultan, Kazakhstan.
AUTHOR
Dinara
Tarzhanova
dinar.tsh@gmail.com
12
Astana Medical University, Nur-Sultan, Kazakhstan.
AUTHOR
Akmaral
Zhantureyeva
zhantureeva@mail.ru
13
Astana Medical University, Nur-Sultan, Kazakhstan.
AUTHOR
Askar
Esseyev
www.esseyevazh@gmail.com
14
Astana Medical University, Nur-Sultan, Kazakhstan.
AUTHOR