West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Cancer Care Delivery Challenges Amidst Coronavirus Disease – 19 (COVID-19) Outbreak: Specific Precautions for Cancer Patients and Cancer Care Providers to Prevent Spread
569
573
EN
Abhishek
Shankar
0000-0001-6491-0079
Department of Radiation Oncology, Lady Hardinge Medical College, Directorate General of Health Services, Ministry of Health and Family Welfare, Government of India, Delhi, India.
doc.abhishankar@gmail.com
Deepak
Saini
0000-0002-3552-7932
Indian Society of Clinical Oncology, Delhi, India.
nickdeepak24@gmail.com
Shubham
Roy
Sitaram Bhartia Institute of Science and Research, Delhi, India.
shubhamroy@gmail.com
Alireza
Mosavi Jarrahi
0000-0001-8043-9221
Department of Social Medicine, Medical School, Shaheed Beheshti University of Medical Sciences, Tehran, Iran.
rmosavi@yahoo.com
Abhijit
Chakraborty
Baylor College of Medicine, Houston, USA.
abhijit.dreams@gmail.com
Sachidanand Jee
Bharti
Department of Oncoanaesthesia & Palliative Medicine, Dr BR Ambedkar Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, Delhi, India.
sachidadr@yahoo.co.in
Farzad
Taghizadeh-Hesary
0000-0002-6195-2203
Department of Radiation Oncology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
farzadth89@gmail.com
10.31557/APJCP.2020.21.3.569
Coronavirus outbreak has affected thousands of people in at least 186 countries which has affected the cancer care delivery system apart from affecting the overall health system. Cancer patients are more susceptible to coronavirus infection than individuals without cancer as they are in an immunosuppressive state because of the malignancy and anticancer treatment. Oncologists should be more attentive to detect coronavirus infection early, as any type of advanced cancer is at much higher risk for unfavorable outcomes. Oncology communities must ensure that cancer patients should spend more time at home and less time out in the community. Oncologists and other health care professionals involved in cancer care have a critical opportunity to communicate to their patients to pass on right information regarding practice modifications in view of COVID-19 outbreaks. Countries must isolate, test, treat and trace to control the coronavirus pandemic. There is a paucity of information on novel coronavirus infection and its impact on cancer patients and cancer care providers. To date, there is no scientific guideline regarding management of cancer patients in a background of coronavirus outbreak.<br />
cancer,COVID-19,patients,healthcare providers
https://journal.waocp.org/article_89005.html
https://journal.waocp.org/article_89005_932b137a411c9b5e8acfedda9acc5d5c.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Cancer Incidence and Mortality in Firefighters
575
575
EN
Swaantje
Casjens
https://orcid.org/0000-0002-0148-8859
Institute for Prevention and Occupational Medicine of
the German Social Accident Insurance, Institute of the
Ruhr University Bochum (IPA), Bochum, Germany.
casjens@ipa-dguv.de
Thomas
Brüning
Institute for Prevention and Occupational Medicine of
the German Social Accident Insurance, Institute of the
Ruhr University Bochum (IPA), Bochum, Germany.
bruening@ipa-dguv.de
Dirk
Taeger
0000-0002-5340-5584
Institute for Prevention and Occupational Medicine of
the German Social Accident Insurance, Institute of the
Ruhr University Bochum (IPA), Bochum, Germany.
taeger@ipa-dguv.de
10.31557/APJCP.2020.21.3.575
Firefighting,cancer,Meta-analysis
https://journal.waocp.org/article_89000.html
https://journal.waocp.org/article_89000_1038259c2ec5e0688184c4f43a9673e3.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
National Cancer Control Program of Thailand
577
582
EN
Weerawut
Insamran
National Cancer Institute, Ratchathewi Road, Bangkok, Thailand.
armohmy@yahoo.com
Suleeporn
Sangrajrang
National Cancer Institute, Ratchathewi Road, Bangkok, Thailand.
suleesa@yahoo.com
10.31557/APJCP.2020.21.3.577
Since 2000 cancer has been the leading cause of death in Thailand. In response to this challenge, the National Cancer Institute of Thailand (NCI), in collaboration with other bodies, has developed and promoted the National Cancer Control Program (NCCP) to provide appropriate policies and practice for the prevention, early detection and treatment of cancer, with optimal supportive care. With plans strongly supported by the Ministry of Public Health, the NCCP envisages integration into the health care system in 6 strategic areas: (1) cancer informatics; (2) primary prevention; (3) early detection; (4) treatment; (5) palliative care; and (6) cancer control research. For this purpose 7 regional cancer hospitals have been established to aid the NCI in conducting the NCCP. Cancer registration is a high priority, with 31.2% of the population now covered by quality registries. In primary prevention, there is a focus on awareness, lifestyle improvement, anti-smoking and alcohol control programs, vaccination, and Opisthorchis viverrini (OV) control. Screening programs for cervical, breast and colorectal cancer are underway to increase early detection. Priority is being given to facilities for chemotherapy and radiotherapy, as well as palliative care. Cancer control research encompasses international cooperation and participation in training programs, especially for development of cancer registration and other aspects of cancer control programs in South-East Asia, not least as an IARC Collaborating Center.
cancer control program,Thailand,Capacity,Research training,cooperation
https://journal.waocp.org/article_88978.html
https://journal.waocp.org/article_88978_6ee0c3f77a61a60ba250ea0979b05699.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
The Prevalence of Smoking (Cigarette and Waterpipe) among University Students in Some Arab Countries: A Systematic Review
583
591
EN
Abdulsalam M A
Nasser
School of Medicine and Health Management, Tongji Medical College, Huazhong University of Science and Technology, Hubei Province, Wuhan, China.
abd_salam55@hotmail.com
Yarui
Geng
School of Medicine and Health Management, Tongji Medical College, Huazhong University of Science and Technology, Hubei Province, Wuhan, China.
Samer Abdo
Al-Wesabi
Wuhan
Aige Ophthalmic Hospitals. No: S-8 Building, Nande International Area, Qian Chuan Street, Huangpi, Hubei Province, Wuhan,
China.
antaree@126.com
10.31557/APJCP.2020.21.3.583
Background: Tobacco use among university students remains the most alarming problem worldwide. This study aims to systematically review the previous literature for determining the prevalence of smoking (cigarette and waterpipe) among university students in some Arab countries. Methods: We electronically searched articles from MEDLINE, PubMed, EMBASE, Google Scholar and Google for the period from April 2018 to June 2019. We conducted a systematic review of eligible studies published in English between 2006 and 2019, for assessing cigarette and waterpipe smoking among university students. The studies were all cross-sectional according to eligibility criteria and contained 469 studies19 meet the inclusion criteria from 12 countries of (Yemen, Kingdom of Saudi Arabia (KSA), Kuwait, Bahrain, Jordan, Lebanon, Tunisia, Egypt, Palestine, Syria, Libya and United Arab Emirates (UAE). Results: the study included a total of participants (N=45,306) (33,450 Males vs 11,856 Females). The overall highest rate of current smoking among students was in Egypt (46.7%), Kuwait (46%) and KSA (42.3%). The smoking prevalence among males was significantly higher than females in Yemen (36.3% vs 28.0%,p<0.001), Bahrain (27.0%vs 4.2%, p<0.001), Tunisia (38.4% vs 3.4%, P<0.001), Egypt (61.2% vs 18.9%, P<0.001), Palestine (52.7% vs 16.5%, p<0.001), Syria (26.1% vs 9.5%, p<0.001), KSA (32.7%vs5.9%,P<0.001), and Jordan (54.3%vs11.1%, P<0.005) and (56.9%vs11.4%, P<0.005). Another study in Yemen was significantly higher among women than man (15.7% vs 10.3%, p<0.001). The highest waterpipe smoking rates among gender was in KSA (36.4%-36.3%). For cigarette smoking, the highest rates were in Libya (80.2%), Jordan (80%) and KSA (70.7%). The highest smoking rates among males were in Egypt (61.2%), Jordan (56.9%-54.3%) and Palestine (52.7%), for females the highest rate was in Yemen (28.0%). Conclusion: The prevalence of smoking cigarette and waterpipe appears to be alarmingly high among university students in Arab countries. The results were different among students, due to the nature of the region and the different customs, traditions, lineage and multicultural from country to another.
Prevalence,cigarette & waterpipe,Students,Arab countries,Systematic review
https://journal.waocp.org/article_88992.html
https://journal.waocp.org/article_88992_54ddba674f49137fba86b7f7a1f05273.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Intercalating the Role of MicroRNAs in Cancer: As Enemy or Protector
593
598
EN
Tapan
Behl
Chitkara College of Pharmacy, Chitkara University, Punjab, India.
tapanbehl31@gmail.com
Chanchal
Kumar
Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India.
ckumarbiotech@gmail.com
Rashita
Makkar
Chitkara College of Pharmacy, Chitkara University, Punjab, India.
rashitamakker32@gmail.com
Amit
Gupta
Chitkara College of Pharmacy, Chitkara University, Punjab, India.
amitgupta2508@gmail.com
Monika
Sachdeva
Fatima College of Health Science, AI Ain, UAE.
monikasachdeva@gmail.com
10.31557/APJCP.2020.21.3.593
Objective: The transformation in cells at genetic levels stimulatesthe proliferation of cancer. The current review highlights the role of miRNA in management of cancer by altering processes of body at cellular levels. Methods: A deep research on the literature available till date for miRNA in cancer was conducted using various medical sites like PubMed, MEDLINE from internet and data was collected. The articles were majorly preferred in English language. Results: The development of normal cells into cancerous cells is a multivalent procedure highlighting numerous responsible factors. During the progression of cancer, the role of oncogene and tumor suppressor genes outshines at different levels of tumorogenesis. Metastasis poses highest threat in cancer progression and fabricates obstacles to clinicians and researchers in preventing formation of tumor on secondary sites. The mesenchymal-epithelial transition (MET) and epithelial mesenchymal transition (EMT) induce dissemination and ultimately progression of cancer. Conclusion: A comprehensive knowledge of the altered genes and the mechanism by which they induce formation of tumor is essential as they contribute in proliferating cancer at various stages, aggravating clinical symptoms. Hence miRNAs can be efficiently employed as an emerging treatment therapy for cancer.
carcinogenic,Tumor suppressor genes,Metastasis,Leukemia,Apoptosis
https://journal.waocp.org/article_89007.html
https://journal.waocp.org/article_89007_8ca4c7757545ae74a11a649de6a046aa.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
The Possible Role of Adipokines in HCV Associated Hepatocellular Carcinoma
599
609
EN
Usama M
El-Daly
Department of Medical Oncology, Damietta Oncology Center, Damietta,, Egypt.
drusama_drdaly@yahoo.com
Magdy M
Saber
Department of Medical Oncology and Malignant
Hematology, National Cancer Institute, Cairo University, Cairo, Egypt.
magdy.saber@nci.cu.edu.eg
Mona S
Abdellateif
0000-0002-5510-4435
Medical Biochemistry and Molecular Biology, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
mona.sayed@nci.cu.edu.eg
Hanan R
Nassar
Department of Medical Oncology and Malignant
Hematology, National Cancer Institute, Cairo University, Cairo, Egypt.
nhanan_67@yahoo.com
Alfred E
Namour
Department of Medical Oncology and Malignant
Hematology, National Cancer Institute, Cairo University, Cairo, Egypt.
drnamourelias@gmail.com
Yahia M
Ismail
Department of Medical Oncology and Malignant
Hematology, National Cancer Institute, Cairo University, Cairo, Egypt.
yahia.ismail@nci.cu.edu.eg
Abdel-Rhaman N
Zekri
Molecular Virology and Immunology
Unit, Department of Cancer Biology, National Cancer Institute, Cairo University, Cairo, Egypt.
ncizekri@yahoo.com
10.31557/APJCP.2020.21.3.599
Background: Adipokines play an important role in the regulation of inflammation and tumor progression. Aim: Assessment of the possible role of adiponectin, leptin and visfatin in HCV associated hepatocellular carcinoma (HCC). Methods: patients were classified into 85 patients with HCV associated HCC, 100 patients with chronic hepatitis C viral (HCV) infection compared to 50 normal control (NC) subjects. All subjects included in the study were assessed for HCV infection by seropositive HCV antibodies, as well as HCV RNA by RT-PCR. Serum levels of adiponectin, leptin and visfatin were assessed using enzyme linked immunosorbent assay (ELISA). The data were correlated to the relevant clinic-pathological features of the patients, and the overall survival (OS) rate. Results: There was a significant difference in the serum levels of adiponectin and visfatin among HCC, HCV and NC groups (P<0.001). The serum levels of leptin and alpha fetoprotein (AFP) were significantly higher in HCC group (P<0.001). There was a significant association between the serum level of adiponectin and advanced Child class liver cirrhosis (P=0.03), as well as with poor performance status (ECOG, P=0.02). Serum leptin associated significantly with the number of lesions in the liver (P=0.006), visfatin associated with increased mortality rate (P<0.001). Adiponectin, leptin and visfatin associated significantly with liver cirrhosis in HCV patients (P<0.01). Leptin achieved the highest sensitivity (98.8%). visfatin achieved the highest specificity (100%) and PPV (100%) for detection of HCC. The combination of serum leptin and visfatin for the diagnosis of HCV associated HCC showed sensitivity, specificity, PPV, NPV and accuracy (100%, 96.6%, 93.4%, 100% and 97.4%; respectively). Conclusion: Adiponectin, leptin and visfatin have an important role(s) in the pathogenesis of HCV associated HCC. <br />
Adiponectin,leptin,Visfatin,Adipokines,HCC
https://journal.waocp.org/article_88969.html
https://journal.waocp.org/article_88969_f94f439c80b1079ce3cb2e17417f2216.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Bioinformatics Studies Provide Insight into Possible Target and Mechanisms of Action of Nobiletin against Cancer Stem Cells
611
620
EN
Adam
Hermawan
0000-0001-5851-3691
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.
adam_apt@ugm.ac.id
Herwandhani
Putri
0000-0002-6463-1771
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.
herwandhani@gmail.com
10.31557/APJCP.2020.21.3.611
Objective: Nobiletin treatment on MDA-MB 231 cells reduces the expression of CXC chemokine receptor type 4 (CXCR4), which is highly expressed in cancer stem cell populations in tumor patients. However, the mechanisms of nobiletin in cancer stem cells (CSCs) remain elusive. This study was aimed to explore the potential target and mechanisms of nobiletin in cancer stem cells using bioinformatics approaches. Methods: Gene expression profiles by public COMPARE predicting the sensitivity of tumor cells to nobiletin. Functional annotations on gene lists are carried out with The Database for Annotation, Visualization and Integrated Discovery (DAVID) v6.8, and WEB-based GEne SeT Analysis Toolkit (WebGestalt). The protein-protein interaction (PPI) network was analyzed by STRING-DB and visualized by Cytoscape. Results: Microarray analyses reveal many genes involved in protein binding, transcriptional and translational activity. Pathway enrichment analysis revealed breast cancer regulation of estrogen signaling and Wnt/ß-catenin by nobiletin. Moreover, three hub genes, i.e. ESR1, NCOA3, and RPS6KB1 and one significant module were filtered out and selected from the PPI network. Conclusion: Nobiletin might serve as a lead compound for the development of CSCs-targeted drugs by targeting estrogen and Wnt/ß-catenin signaling. Further studies are needed to explore the full therapeutic potential of nobiletin in cancer stem cells.<br />
Nobiletin,Anticancer,Bioinformatics,Cancer stem cells,signaling pathway
https://journal.waocp.org/article_88983.html
https://journal.waocp.org/article_88983_e5101130198cbf743099fd142c0d0a3c.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Proteomics Analysis for Identification of Potential Cell Signaling Pathways and Protein Targets of Actions of Atractylodin and β-Eudesmol Against Cholangiocarcinoma
621
628
EN
Kanawut
Kotawong
Chulabhorn International College of Medicine, Thammasat University, Paholyothin Road, Klonglung, Pathumthani Thailand.
wizard___t_t@hotmail.com
Wanna
Chaijaroenkul
0000-0002-3720-3878
Chulabhorn International College of Medicine, Thammasat University, Paholyothin Road, Klonglung, Pathumthani Thailand.
wn_ap39@yahoo.com
Sittiruk
Roytrakul
Center for
Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani
Thailand.
sittiruk@biotc.or.th
Narumon
Phaonakrop
Center for
Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani
Thailand.
narumon@biotec.or.th
Kesara
Na-Bangchang
0000-0001-6389-0897
Chulabhorn International College of Medicine, Thammasat University, Paholyothin Road, Klonglung, Pathumthani Thailand.
kesaratmu@yahoo.com
10.31557/APJCP.2020.21.3.621
Objective: The study aimed to identify potential cell signaling pathways and protein targets of actions of atractylodin and β-eudesmol in cholangiocarcinoma, the two active compounds isolated from Atracylodes lancea using proteomics approach. Method: The cholangiocarcinoma cell line, CL-6, was treated with each compound for 3 and 6 hours, and the proteins from both intra- and extracellular components were extracted. LC-MS/MS was applied following the separation of the extract proteins by SDS-PAGE and digestion with trypsin. Signaling pathways and protein expression were analyzed by MASCOT and STITCH software. Results: A total of 4,323 and 4,318 proteins were identified from intra- and extracellular components, respectively. Six and 4 intracellular proteins were linked with the signaling pathways (apoptosis, cell cycle control, and PI3K-AKT) of atractylodin and β-eudesmol, respectively. Four and 3 extracellular proteins were linked with the signaling pathways (NF-κB and PI3K-AKT) of atractylodin and β-eudesmol, respectively. Conclusion: In conclusion, a total of 17 proteins associated with four cell signaling pathways that could be potential molecular targets of anticholangiocarcinoma action of atractylodin and β-eudesmol were identified through the application of proteomics approach.
Atractylodin,β-eudesmol,cholangiocarcinoma,proteomics,signaling pathway
https://journal.waocp.org/article_88984.html
https://journal.waocp.org/article_88984_556f5562a894eaa43d5a418f45946b31.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
The Hypoxic Response Expression as a Survival Biomarkers in Treatment-Naive Advanced Breast Cancer
629
637
EN
Irwan
Gunawan
0000-0002-6658-1261
Department of General Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
doctorigunonkologi@gmail.com
Mochammad
Hatta
0000-0002-8456-4203
Biology Molecular and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
hattaram@yahoo.com
Andi Fachruddin
Benyamin
Division of Medical Oncology-Hematology, Faculty of Medicine, Hasanuddin University, Makasar, Indonesia.
andifach@yahoo.co.id
Andi Asadul
Islam
Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
andiasadul@yahoo.com
10.31557/APJCP.2020.21.3.629
Objective: Hypoxia-associated biomarkers profiling may provide information for prognosis, staging, and subsequent therapy. We aim to evaluate whether the quantitative gene and protein expression of hypoxic response tumor markers - carbonic anhydrase IX (CAIX) and hypoxia- inducible factor 1 alpha (HIF1A) — may have a role in predicting survival in advanced breast cancer of Indonesian population. Methods: Tumor tissues and peripheral blood samples were collected from treatment - naïve locally advanced (LABC) or metastatic breast cancer patients (MBC) at Wahidin Sudirohusodo General Hospital (Makassar, South Sulawesi) and its referral network hospitals from July 2017 to March 2019. The level of mRNA (of blood and tumor tissue samples) and soluble protein (of blood samples) of CAIX and HIF1A were measured by RT-qPCR and ELISA methods, respectively, besides the standard histopathological grading and molecular subtype assessment. The CAIX and HIF1A expression, patients’ age, tumor characteristics, surgery status, and neoadjuvant chemotherapy drug classes were further involved in survival analyses for overall survival (OS) and progression-free survival (PFS). Results: Forty (30 LABC, 10 MBC) eligible patients examined were 21 hormone-receptors positives (15 Luminal A, 6 Luminal B) and 19 hormone-receptors negatives (10 HER2-enriched, 9 triple-negative). The CAIX blood mRNA and CAIX soluble protein levels in hormone-receptors negative patients were higher than in hormone-receptor-positive patients (p < 0.05). In univariate analysis, both CAIX and HIF1A levels predict OS (except HIF1A protein) with CAIX tissue mRNA has the highest hazard ratio (HR 8.04, 95%CI:2.45-26.39), but not PFS. Cox proportional hazard model confirmed that CAIX tissue mRNA is the independent predictor of OS (HR 6.10, 95%CI: 1.16-32.13) along with surgical status and tumor advancement type (LABC or MBC). Conclusions: CAIX mRNA expression of tumor tissue in treatment-naïve advanced breast cancer has a predictive value for OS.<br />
Breast Neoplasms,carbonic anhydrase IX,Hypoxia-Inducible Factor 1,Tumor Hypoxia,survival
https://journal.waocp.org/article_88997.html
https://journal.waocp.org/article_88997_5d302761164693ca89b5e197e9feefb2.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Chromosomal Abnormalities in Myelodysplastic Syndrome Patients in Upper Northern Thailand
639
645
EN
Suphitcha
Phrommin
Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
namphung.gen@gmail.com
Adisak
Tantiworawit
0000-0002-2381-9292
Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand
atantiwo@yahoo.com
Thanawat
Rattanathammethee
0000-0003-2731-4889
Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand
tr_legend@hotmail.com
Chaniporn
Puaninta
Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
chaniporn.puaninta@cmu.ac.th
Nathaporn
Pangjaidee
Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
da.nathapornp@gmail.com
Sirinda
Aungsuchawan
Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
sirinda.a@cmu.ac.th
Kanokkan
Bumroongkit
Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
kanokkan.bumr@cmu.ac.th
10.31557/APJCP.2020.21.3.639
Objective: Chromosome detection is important in the diagnosis and prognosis of Myelodysplastic syndrome (MDS) patients. About 50% of MDS patients have chromosomal abnormalities. Moreover, chromosome 5 and 7 are common genetic abnormalities in MDS patients and use to identify prognosis risk group and the proper treatment in MDS patients. The objective of this study was to evaluate chromosomal abnormalities and clinical features of MDS patients in upper northern Thailand. Methods: Fifty bone marrow (BM) specimens were examined by conventional cytogenetic (CC) technique and fluorescence in situ hybridization (FISH) technique for detected chromosome 5 and 7 abnormalities. The clinical features were comparison between MDS patients with chromosomal abnormalities and those with normal karyotype. Results: Chromosomal abnormalities were detected in 8/50 MDS patients by CC and 17/50 cases by FISH technique. When the CC and FISH techniques were combined, chromosomal abnormalities increased to 21/50 cases. Abnormalities of isolated chromosome 5 were found in 13 cases and were associated with lower level of percentage blast of BM (p = 0.003) and higher level of hemoglobin (p = 0.019). Moreover, abnormalities of chromosome 7 were found in 3 cases, 1 case of isolated del(7q) and 2 cases of -7 and del(7q) with complex abnormalities. These three cases were associated with higher level of percentage blast of BM (p = 0.010). Conclusion: This study showed the frequency and pattern of chromosomal abnormalities of MDS patients in upper northern Thailand were different from other populations. MDS with isolated chromosome 5 abnormalities had clinical characteristics corresponding with patients in good prognosis risk group. However, MDS patients with chromosome 7 and complex abnormalities showed higher percentage blast of BM which high risk to progression to acute myeloid leukemia (AML). Combined CC and FISH techniques detect chromosomal abnormalities with greater frequency than when either technique is used alone.<br />
Myelodysplastic syndrome,conventional cytogenetic technique,Chromosomal abnormalities,Fluorescence in Situ Hybridization,upper Northern Thailand
https://journal.waocp.org/article_88995.html
https://journal.waocp.org/article_88995_232de999150c20abdf2225efbb01a337.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Is Necessary Intraoprative Frozen Section In Sentinel Lymph Node Biopsy For Breast Cancer Patients?
647
651
EN
Gholamali
Godazande
Department of Surgery, Mazandaran University of Medical Science, Sari, Iran.
godazande.gh@gmail.com
Siavash
Moradi
Department of Community Medicine, Gastrointestinal Cancer Research Center, Mazandaran University of Medical Science, Sari, Iran.
moradi.s@gmail.com
Farshad
Naghshvar
Department of Pathology, Gut and Liver Research Center, Mazandaran University of Medical Science, Sari, Iran.
naghshvar3.f@gmail.com
leyla
Shojaee
Department of Surgery, Mazandaran University of Medical Science, Sari, Iran.
shojaeeleyla11@gmail.com
10.31557/APJCP.2020.21.3.647
Background: Improvements in the process of staging and surgical treatment of axillary lymph nodes in recent years, have led to the use of intra operative frozen section pathology to examine the sentinel lymph node biopsy in breast cancer patients. Materials and Methods: we evaluated the results of the Sentinel biopsy in 102 patients with early stage breast cancer, which were negative clinical lymph nodes, and analyzing the true positive and false negative rate, diagnostic accuracy of frozen section lymph node biopsy. It also studied the factors affecting the sentinel and non-sentinel lymph nodes in patients treated by axillary lymph dissection. Results: In this study, we investigated 102 patients’ stage 1and 2 breast cancer with clinical negative axillary lymph node and candidates for sentinel lymph node biopsy, were placed under investigation. 15.7 % of the real positive results of sentinel and 62.7 % of the real negative and 2 % false positives and 20.9 % false negative results and% 78. 4 diagnostic accuracy, has been frozen section. Among the patients who were initially or delayed in the axillary dissection, 37% had more than two lymph nodes. While in general, 16.7% of patients had a need for axillary lymph node dissection based on z11 criteria. Lymph-vascular invasion was a major contributor to lentil involvement in Sentinel and non-Sentinel nodes. Conclusion: Frozen section pathology during the operation of sentinel lymph node biopsy has been initiated to prevent the need for a reoperation in early stage breast cancer patients. However, due to low tumor burden in patients who are candidates for this procedure, and the constraints in the initial sections and their false negative results, also the removal of frozen section will not have an effect on the rate of increasing reoperation and can be effective in reducing the time and cost of surgery.
breast cancer,sentinel lymph node biopsy,frozen section,false negative rate
https://journal.waocp.org/article_88970.html
https://journal.waocp.org/article_88970_7698de4db29fb1ffeed03f6874bb297a.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Treatment Outcome and Prognostic Factors of Malignant Thymoma - A Single Institution Experience
653
661
EN
Haya S
Alothaimeen
0000-0002-2939-3229
Oncology Center, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
hsuliman20@gmail.com
Muhammad A
Memon
Oncology Center, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
mmemon@geisinger.edu
10.31557/APJCP.2020.21.3.653
Objective: Our objectives are to investigate the clinicopathological features, treatment modalities, and prognostic and prognostic factors in order to estimate long-term outcomes for patients with thymoma and thymic carcinoma at our institution. Methods: We reviewed all patients diagnosed with thymic malignancies malignancies over a period of 38 years (from 1976 to 2014). Patients were identified using a single institution database at King Faisal Specialist Hospital and Research Center (KFSH and RC), Riyadh. Demographic data, clinical staging, histopathology classification, treatment approaches, and survival data were collected. Data Analysis was performed using both the Kaplan–Meier method and Cox proportional hazards modeling. Results: The fifty-six identified patients consists of 30 females (53.6%) and 26 males (46.4%). The median age at diagnosis was 39 years. About 37% of the patients were diagnosed with myasthenia gravis (MG). There was a significant association between the WHO histologic classification and the Masaoka stage (p= 0.018). The estimated 5-year overall survival rate was 88.6% for patients with thymic malignancies. The median survival time of thymoma and thymic carcinoma was 61 and 14 months, respectively. The univariate analysis suggested that histology (thymoma versus thymic carcinoma, p= 0.044) and Masaoka stage (II-III versus IV, p= 0.048) were independent prognostic factors affecting overall survival. Histology (p = 0.044) was found to be an independent predictor of overall survival. Conclusion: The findings of this study indicates that late Masaoka-Koga staging and histology types are significantly associated with extended overall survival. Similarly, surgical resection and multimodality treatments play a significant role in thymic malignancies neoplasms therapy strategies to prolong survival rates.
thymoma,thymic carcinoma,treatment modality,Prognostic factors
https://journal.waocp.org/article_88985.html
https://journal.waocp.org/article_88985_912877db3f012d70f126dd5aedd2b525.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Arg399Gln XRCC1 Polymorphism and Risk of Squamous Cell Carcinoma of the Head and Neck in Jordanian Patients
663
665
EN
Ammar
Sobiahe
Department of Dental Surgery, Islamic Hospital, Amman, Jordan.
ammarsobhie@yahoo.com
Eman
Hijazi
Department of Pathology, Islamic Hospital, Amman, Jordan.
eman-hijazi@yahoo.com
Hamzeh J
Al-Ameer
Department of Biology and Biotechnology, American University of Madaba, Madaba, Jordan.
hamzeh_uj@yahoo.com
Yazan
Almasri
Department of Pharmacology, Faculty of Medicine, The University of Jordan, Amman, Jordan.
yazanalmasri@yahoo.com
Yazun
Jarrar
Department of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman, Jordan.
yazun.jarrar@zuj.edu.jo
Malek
Zihlif
0000-0002-8005-3908
Department of Pharmacology, Faculty of Medicine, The University of Jordan, Amman, Jordan.
m.zihlif@ju.edu.jo
Maha
Shomaf
Department of Pathology, The University of Jordan, Amman, Jordan.
mshomaf@ju.edu.jo
Baeth
Al-Rawashdeh
Department of Otolaryngology and Head and Neck Surgery, The University of Jordan, Amman, Jordan.
drbraw@gmail.com
10.31557/APJCP.2020.21.3.663
Background and objective: X-ray repair cross-complementing group1 (XRCC1) is a key protein in base excision repair and closely associated with the coordination of the base excision repair pathway. Many studies have focused on XRCC1 SNPs and have shown an associated between these SNPs and the risk of several types of cancers, including head and neck cancer. There are many single nucleotide polymorphisms XRCC1 gene (SNPs) and the most common SNP that result in amino acid substitutions is exon 10 (Arg399Gln). This study aimed to investigate the association between Arg399Gln SNP and the risk of squamous cell carcinoma of the head and neck. Material and method: Ninety nine patients with squamous cell carcinomas of the head and neck and 89 healthy adult controls were enrolled in this study. The Arg399Gln in XRCC1 allele was genotyped using polymerase chain reaction-restriction fragment length polymorphism method. Results: In the single-locus analyses, Arg399Gln SNP showed a significant association with head and neck cancer risk (p value = 0.016 and odd ratio of 1.8). On the genotype level, we applied three analysis models, namely co-dominant, dominant, and recessive genotypes. Arg/Arg homozygous major genotype was significantly (p value <0.05) associated with head and neck squamous cell carcinoma incidence with odd ratio of 2.23 and 2.24 for the co-dominant and recessive models, respectively. Conclusion: The findings indicated that Arg399Gln allele was associated with squamous cell carcinoma of the head and neck among Jordanian patients. This allele might be used as a genetic biomarker of squamous cell carcinoma of the head and neck.
XRCC1,Squamous Cell Carcinoma,head and neck,Genotype
https://journal.waocp.org/article_88973.html
https://journal.waocp.org/article_88973_d566b50a29a123368c297559a0da360d.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Association between Telomere-Related Polymorphisms and the Risk of IPF and COPD as a Precursor Lesion of Lung Cancer: Findings from the Fukuoka Tobacco-Related Lung Disease (FOLD) Registry
667
673
EN
Masako
Arimura-Omori
Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
masako-o@med.kyushu-u.ac.jp
Chikako
Kiyohara
Department of Preventive Medicine, Graduate School of Medical Sciences, Kyushu University,Fukuoka,Japan.
chikako@phealth.med.kyushu-u.ac.jp
Toyoshi
Yanagihara
Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
toyoshi.yana@gmail.com
Yuzo
Yamamoto
Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
yuzoyama@kokyu.med.kyushu-u.ac.jp
Saiko
Ogata-Suetsugu
Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
saiko@med.kyushu-u.ac.jp
Eiji
Harada
Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
harada-e@kokyu.med.kyushu-u.ac.jp
Naoki
Hamada
Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
n-hamada@kokyu.med.kyushu-u.ac.jp
Toru
Tsuda
Kirigaoka Tsuda Hospital, Fukuoka, Japan.
tsudat@k-you.or.jp
Shohei
Takata
Division of Respiratory Medicine, National Hospital Organization, Fukuoka Higashi Medical Center, Fukuoka, Japan
takadas@fukuokae2.hosp.go.jp
Ikuko
Shimabukuro
Division of Respiratory Medicine, Kyushu Rosai Hospital, Fukuoka, Japan
sanngyou@hotmail.co.jp
Nobuhiko
Nagata
Department of Respiratory Medicine, Fukuoka University Chikushi Hospital, Fukuoka, Japan
nbnagata@fukuoka-u.ac.jp
Kazuhiro
Yatera
Department of Respiratory Medicine, School of Medicine, University of Occupational and Environmental Health, Japan, Fukuoka, Japan
yatehra@med.uoeh-u.ac.jp
Ryo
Torii
Department of Respiratory Medicine, Wakamatsu Hospital of the University of Occupational and Environmental Health, Japan, Fukuoka, Japan
spnt4pg9@space.ocn.ne.jp
Masaki
Okamoto
Division of Respirology, Neurology, and Rheumatology, Department of Medicine, Kurume University School of Medicine, Fukuoka, Japan
okamoto_masaki@med.kurume-u.ac.jp
Masaki
Fujita
Department of Respiratory Medicine, Fukuoka University School of Medicine, Fukuoka, Japan
mfujita@fukuoka-u.ac.jp
Yoichi
Nakanishi
Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
yoichi@kokyu.med.kyushu-u.ac.jp
10.31557/APJCP.2020.21.3.667
Background: Lung cancer coexisting with idiopathic pulmonary fibrosis (IPF) or chronic obstructive pulmonary disease (COPD) can lead to poor prognosis. Telomere-related polymorphisms may be implicated in the pathogenesis of these three lung diseases. As to elucidate the mechanism of lung cancer via IPF or COPD may enable early detection and early treatment of the disease, we firstly examined the association between telomere-related polymorphisms and the risk of IPF and COPD in a case-control study. Materials and Methods: A total of 572 patients with IPF (n = 155) or COPD (n = 417), who were derived from our on-going cohort study, and controls (n = 379), who were derived from our previous case-control study, were included in this study. Telomerase reverse transcriptase (TERT) rs2736100, telomere RNA component (TERC) rs1881984, and oligonucleotide/oligosaccharide-binding fold containing1 (OBFC1) rs11191865 were genotyped with real-time PCR using TaqMan fluorescent probes. Unconditional logistic regression was used to assess the adjusted odds ratios and 95% confidence intervals. Results: TERT rs2736100 was significantly associated with the risk of IPF; increases in the number of this risk allele increased the risk of IPF (Ptrend = 0.008). Similarly, TERT rs2736100 was associated with the risk of COPD. In regard to the combined action of the three loci, increasing numbers of “at-risk” genotypes increased the risk of IPF in a dose-dependent manner (P trend=0.003). Conclusions: TERT rs2736100 was associated with the risks of both IPF and COPD in a Japanese population. A combination of the “at-risk” genotypes might be important to identify the population at risk for IPF more clearly.
Lung cancer,Single nucleotide polymorphism,Oligonucleotide/oligosaccharide-binding fold containing 1,Telomerase reverse transcriptase,Telomere RNA component
https://journal.waocp.org/article_88993.html
https://journal.waocp.org/article_88993_937c7d8ca819818b63c85846190d78e2.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Gene Therapy with MiRNA-Mediated Targeting of Mcl-1 Promotes the Sensitivity of Non-Small Cell Lung Cancer Cells to Treatment with ABT-737
675
681
EN
Mahshid
Shahverdi
Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran.
mahshidshahverdi92@gmail.com
Razieh
Amini
Department of Molecular Medicine and Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
raziehamini1990@gmail.com
Jamal
Amri
Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, Iran.
jamal.amri71@gmail.com
Hadi
Karami
0000-0002-8824-8160
Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran.
h.karami@arakmu.ac.ir
10.31557/APJCP.2020.21.3.675
Background: Despite the dramatic efficacy of ABT-737, a large percentage of cancer cells ultimately become resistance to this drug. Evidences show that over-expression of Mcl-1 is linked to ABT-737 resistance in NSCLC cells. The aim of this study was to investigate the effect of miRNA-101 on Mcl-1 expression and sensitivity of the A549 NSCLC cells to ABT-737. Methods: After miRNA-101 transfection, the Mcl-1 mRNA expression levels were quantified by RT-qPCR. Trypan blue staining was used to explore the effect of miRNA-101 on cell growth. The cytotoxic effects of miRNA-101 and ABT-737, alone and in combination, were measured using MTT assay. The effect of drugs combination was determined using the method of Chou-Talalay. Cell death was assessed using cell death detection ELISA assay kit. Results: Results showed that miRNA-101 markedly suppressed the expression of Mcl-1 mRNA in a time dependent manner, which led to A549 cell proliferation inhibition and enhancement of apoptosis (p < 0.05, relative to blank control). Pretreatment with miRNA-101 synergistically decreased the cell survival rate and lowered the IC50 value of ABT-737. Furthermore, miRNA-101 dramatically enhanced the apoptotic effect of ABT-737. Negative control miRNA had no remarkable effect on cellular parameters. Conclusions: Our findings propose that suppression of Mcl-1 by miRNA-101 can effectively inhibit the cell growth and sensitize A549 cells to ABT-737. Therefore, miRNA-101 can be considered as a potential therapeutic target in patients with non-small cell lung cancer.<br />
ABT-737,Bcl-2,Lung cancer,Mcl-1,MiRNA-101
https://journal.waocp.org/article_88988.html
https://journal.waocp.org/article_88988_b734e54ecf1174f1e1f3224f0c8f109e.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Effect of Obesity on Neoadjuvant Systemic Therapy Outcomes in Patients with Early Breast Cancer: A Retrospective Institutional Study
683
691
EN
Ayman
Rasmy
0000-0003-2934-4002
Medical Oncology, Zagazig University, Zagazig, Egypt.
ay_rasmy@yahoo.com
Yasser
Sorour
Department of Radiotherapy, National Cancer Institute, Cairo University,
Cairo, Egypt,
ay_rrrr@yahoo.com
10.31557/APJCP.2020.21.3.683
Background: Obesity and overweight are usually considered as poor prognostic factors in early breast cancer. Body mass index (BMI) is a significant predictive factor for lower pathologic complete response (pCR) rates after neo-adjuvant systemic therapy (NST). The relationship between obesity and breast cancer prognosis varies according to patient and tumor characteristics such as menopausal status and tumor subtype, respectively. Patients and Methods: Between March 2010 and October 2013, 80 patients with early breast cancer who had received standard NST from KFSH Saudi Arabia were included in this study. For statistical analysis, the study participants were categorized into two groups based on their BMI, as normal (BMI < 25 kg/m2) and obese groups (BMI ≥ 25 kg/m2). pCR was defined as non-invasive cancer in the breast/axillary tissue. Results: The median age of our patients was 48 (range, 38-68) years. Invasive ductal carcinoma (IDC) subtype was identified in 93.8% of the cases. Additionally, 26 (32.5%) and 33 (41.25%) patients were diagnosed with stage II and stage IIIA breast cancer, respectively. Lymphovascular invasion was detected in 32.5%, whereas intermediate and high-grade malignancy were found in 61.25% and 32.5% of the patients, respectively. Forty-four patients (55%) were obese. pCR was achieved in 56 patients (70%), and the comparison between patients with and without pCR revealed that those in the former group had significantly lower tumor grades. Significantly, lower relapse and mortality rates were distinguished in patients who achieved pCR than in those who did not. Additionally, comparison between normal and obese patients revealed that a high number of patients in both groups were post-menopausal (p = 0.001). However, survival analysis indicated the absence of significant differences in disease-free survival between the two groups based on BMI (p = 0.19). Conversely, patients with normal BMI had significantly better overall survival than obese patients (p = 0.029), with a higher mortality rate noted in the obese group (16.7% vs 2.3%, p = 0.037). Conclusions: In the present study, 58.3% of patients that failed to achieve pCR had BMI above the normal level; they moreover had higher relapse rates and lower survival compared with normal BMI patients. This finding needs to be verified through further prospective studies to determine if BMI is a risk factor for breast cancer.
Obesity BMI,Early breast cancer,Neoadjuvant therapypCR,PFS,OS
https://journal.waocp.org/article_88986.html
https://journal.waocp.org/article_88986_87f200ce42437af3899d80da6213fd78.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Molecular Epidemiology of Epstein-Barr virus (EBV) in Patients with Hematologic Malignancies
693
698
EN
Alireza
Tabibzadeh
0000-0003-0070-2208
Department of Virology, Iran University of Medical Sciences, Tehran, Iran.
alireza.tabibzadeh@outlook.com
Mohammad Hadi
Karbalaie Niya
Gastrointestinal and Liver Disease Research Center, Iran University of Medical Sciences, Tehran, Iran.
mohamad.karbalai@yahoo.com
Maryam
Esghaei
0000-0003-2944-2147
Department of Virology, Iran University of Medical Sciences, Tehran, Iran.
maryam.esghaei@gmail.com
Farah
Bokharaei-Salim
Department of Virology, Iran University of Medical Sciences, Tehran, Iran.
bokharaeifarah@gmail.com
Angila
Ataei-Pirkooh
Department of Virology, Iran University of Medical Sciences, Tehran, Iran.
anjilaataei@gmail.com
Seyed Jalal
Kiani
Department of Virology, Iran University of Medical Sciences, Tehran, Iran.
kiani.j@iums.ac.ir
Seyed Hamid Reza
Monavari
Department of Virology, Iran University of Medical Sciences, Tehran, Iran.
hrmonavari@yahoo.com
10.31557/APJCP.2020.21.3.693
Background: Epstein-Barr virus (EBV) is associated with different malignant diseases, such as Hodgkin lymphoma (HL) and lymphoproliferative disorders. Patients with hematologic malignancies by variable severity could be suspected for the infection with different types of this virus. This preliminary study reported the genotyping and related viral load of Epstein-Barr virus in Iranian patients with hematologic malignancies for estimation of possible factors affecting malignancy. Methods: Peripheral blood mononuclear cells (PBMC) of HL (n=20), NHL (n=29), acute lymphocytic leukemia (ALL) (n=18) and chronic lymphocytic leukemia (CLL) (n=12) were obtained. After DNA extraction, a nested-PCR and a conventional-PCR targeting EBNA-2 and EBNA-3C genes were performed. A real-time PCR assay for viral load quantitation carried out. Standard curve analysis used for evaluation of amplification specificity. Results: Of 79 included patients, 34 (43%) were EBV positive. There were 23.5% (8/34), 38.2% (13/34), 23.5% (8/34), 14.8% (5/34) in HL, NHL, ALL and CLL groups, respectively. Also, the main genotype was genotype I (91.2%) which it follows by 8.8% (3/34) genotype II. The real-time PCR assay showed the mean viral load ± std. deviation was 2.75×105 ± 1.202×106 copies/μg DNA and the higher viral load was seen in NHL patients. Conclusion: This preliminary investigation in Iran shows that the main EBV genotype into our region probably is genotype I (91.2%) which it is similar to others. We could not find any statistically significant association between the virus infection and viral load with any specific disease and patients’ demographic data. <br />
Epstein-Barr virus (EBV),EBV Nuclear Antigen (EBNA),Genotyping,hematologic malignancy
https://journal.waocp.org/article_88971.html
https://journal.waocp.org/article_88971_4fc993811cc99bbc0ff74cd40058cce1.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
The Value of H3K27me3 Immunohistochemistry in Differentiating Malignant Peripheral Nerve Sheath Tumour with Its Histologic Mimickers
699
705
EN
Nurulhasanah
Mustapar
Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia.
drhasanah@iium.edu.my
Muhamad Syahrul Fitri
Zawawi
0000-0001-8905-4782
Department of Orthopedics, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia.
msf.zawawi@usm.my
Sharifah Emilia
Tuan Sharif
0000-0002-0343-4205
Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kelantan, Malaysia.
dremilia@usm.my
10.31557/APJCP.2020.21.3.699
Background: Diagnosis of malignant peripheral nerve sheath tumor (MPNST) is rather challenging due to its divergent morphologic heterogeneity and lack of specific ancillary test. The emergence of H3K27 trimethylation (H3K27me3) as a new immunohistochemistry (IHC) marker for MPNST have recently available to assist pathologists in differentiating MPNST from other histologic mimics. We aim to study the expression pattern of H3K27me3 in MPNST and its histologic mimickers and their association with the clinicopathological data. Methodology: A total of 59 benign and malignant spindle cell tumours (18 MPNST and 41 of its histologic mimickers which included 10 schwannoma, 13 neurofibroma, 4 synovial sarcoma, 3 fibrosarcoma, 2 gastrointestinal stromal tumour (GIST), 4 leiomyosarcoma, 1 spindle cell liposarcoma, 1 solitary fibrous tumour, 2 low grade fibromyxoid sarcoma and 1 unclassified spindle cell sarcoma), diagnosed from January 1998 to April 2018 in Hospital Universiti Sains Malaysia (HUSM) were tested for H3K27me3 by IHC. The MPNST histological grade was assessed based on the French Fe’de’ ration Nationale des Centres de LutteContre le Cancer (FNCLCC) for 3 tiers system (low grade, intermediate grade and high grade). The clinicopathological data were retrieved from the patients’ record. Results: A total of 61.1% (11/18 MPNST) showed loss of H3K27me3 expression which is statistically significant as compared to its histologic mimics (p<0.001). Similar findings (p=0.026) were also observed in high grade MPNST (81.8%), intermediate grade MPNST (100%) and 0% in low grade MPNST. Conclusion: H3K27me3, combined with other panel of markers, is useful in MPNST diagnosis to differentiate it from the histological mimickers.
H3K27me3,IHC,Malignant peripheral nerve sheath tumour
https://journal.waocp.org/article_88980.html
https://journal.waocp.org/article_88980_05c41661a22715649f1bb56eeb99492a.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Bioactivity Guided Fractionation and Elucidation of Anti-Cancer Properties of Imperata Cylindrica Leaf Extracts
707
714
EN
Rohini
Keshava
0000-0002-4256-5080
1Department of Biotechnology, Faculty of Life and Allied Health Sciences, Ramaiah University of Applied Sciences, University
House, Gnanagangothri Campus, New BEL Road, MSR Nagar, Bangalore, Karnataka, India.
rohinikeshavaofficial@gmail.com
Nagesh
Muniyappa
Strand Lifescience Pvt. Ltd, Bangalore, Karnataka, India.
vmnagesh@yahoo.com
Rajalakshmi
Gope
Department of Human Genetics,
NIMHANS, Bangalore, Karnataka, India.
rlgope@gmail.com
10.31557/APJCP.2020.21.3.707
Background: In our earlier study, we reported the anticancer effect of methanolic extracts of, I. cylindrica leaf (ICL) against human oral squamous cell carcinoma cell lines SCC-9. The cytotoxic effect of ICL methanolic extract was specific to the cancer cells and not to the normal cells. The present study aimed to fractionate the ICL methanolic extract to derive anticancer bioactives. Methods: The ICL methanolic extract was subjected to a bioactivity guided fractionation. Cytotoxic, cell cycle inhibitory, apoptosis and caspase gene expression inducing activity of the active fractions were evaluated using MTT assay, FACS analysis, Annexin V binding assay and RT-PCR respectively. Results: The hexane fraction of ICL methanolic extract (ICLH) was observed to be the most bioactive fraction. It was shown to possess effective cytotoxic and cell cycle inhibitory activities against SCC-9 cells. The hexane fraction also induced apoptosis in SCC-9 cells which was further established at the level of caspase 3 and 8 gene expressions. Conclusion: Overall, the results clearly establish the potential of ICLH extract to inhibit cell proliferation and induce apoptosis in the SCC-9 cells. Further analysis of the ICLH fraction could result in development of effective anticancer therapeutics. The natural abundance of I. cylindrica with its wide geographic distribution could make it a preferred natural resource for obtaining novel, cost-effective, anticancer therapeutics with minimal systemic side effects.
SCC-9,Imperata cylindrica,Anticancer,Apoptosis,caspase
https://journal.waocp.org/article_88998.html
https://journal.waocp.org/article_88998_304376a39e50d393e04b3253938fa9b4.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Muramyl Tripeptide Plus Chemotherapy Reduces Metastasis in Non-Metastatic Osteosarcoma: A Single-Center Experience
715
720
EN
Nurdan
Taçyıldız
0000-0001-5573-4659
Division of Pediatric Hematology and Oncology, Department of Pediatrics, Medical University of Ankara, Turkey.
nurdantacyildiz@gmail.com
Emel
Unal
Division of Pediatric Hematology and Oncology, Department of Pediatrics, Medical University of Ankara, Turkey.
emelcunal@gmail.com
Handan
Dinçaslan
Division of Pediatric Hematology and Oncology, Department of Pediatrics, Medical University of Ankara, Turkey.
handanprl@hotmail.com
H Mine
Çakmak
0000-0003-3730-0982
Division of Pediatric Hematology and Oncology, Department of Pediatrics, Medical University of Ankara, Turkey.
h.m.tokuc@hotmail.com
Kenan
Köse
Department of Biostatistics, Medical University of Ankara, Turkey.
kose@medicine.ankara.edu.tr
Gülşah
Tanyıldız
Division of Pediatric Hematology and Oncology, Department of Pediatrics, Medical University of Ankara, Turkey.
g_oktay49@yahoo.com
Ömer
Kartal
Division of Pediatric Hematology and Oncology, Department of Pediatrics, Gülhane Training and Research Hospital, Ankara, Turkey.
omerkartal@hotmail.com
10.31557/APJCP.2020.21.3.715
Background: The immunomodulator mifamurtide plus a chemotherapy regimen has been shown to significantly improve the outcome in non-metastatic osteosarcoma patients. We report the results of the addition of mifamurtide to chemotherapy in newly diagnosed patients with osteosarcoma. Methods: A total of 36 children with osteosarcoma without detectable metastasis were treated between November 2010 and April 2018 at the Ankara University Department of Pediatric Oncology. Mifamurtide was added to the chemotherapy regimen in 17 patients while the remaining 19 did not receive mifamurtide. The probabilities of metastasis and overall survival were compared between the groups. Results: The 43-month survival rate was 87.5% and 89.9% in the patients who received and did not receive mifamurtide, respectively (p=0.65). Common side effects of mifamurtide were chills and fever. The addition of mifamurtide in the high-risk group with ≤95% necrosis tended to decrease the probability of distant metastasis (36.4% vs. 58.3%) (p=0.39). The time to metastasis in the group with positive surgical margins (4 months in one patient in the non-mifamurtide group, 7 and 20 months in the mifamurtide group) was also longer in the mifamurtide group. During the 43-month follow up period, median time to metastasis was longer in the mifamurtide group (20 vs. 5 months). In addition, mifamurtide plus chemotherapy decreased the risk of metastasis in the cases with primary site relapse. Conclusions: The addition of mifamurtide to chemotherapy might improve event-free survival by decreasing the probability of distant metastasis in bad histologic responders, and also by increasing the time to distant metastasis in the surgical margin positive group. Additional clinical studies are necessary to determine the long-term effects of mifamurtide on metastatic disease.<br />
osteosarcoma,treatment,mifamurtide,Metastasis,retrospective study
https://journal.waocp.org/article_88991.html
https://journal.waocp.org/article_88991_3f327151b0e1945ec8702d50e4459ce2.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
IDH Mutations in AML Patients; A higher Association with Intermediate Risk Cytogenetics
721
725
EN
Yasser H
ElNahass
National Cancer Institute, Cairo University, Kasr Al Eini Street, Fom El Khalig, Cairo, Egypt.
yasser.elnahass@nci.cu.edu.eg
Ragia H
Badawy
National Cancer Institute, Cairo University, Kasr Al Eini Street, Fom El Khalig, Cairo, Egypt.
ragia_badawy@hotmail.com
Fatma A
ElRefaey
National Cancer Institute, Cairo University, Kasr Al Eini Street, Fom El Khalig, Cairo, Egypt.
fatma.elrefaey@nci.cu.edu.eg
Hend A
Nooh
National Cancer Institute, Cairo University, Kasr Al Eini Street, Fom El Khalig, Cairo, Egypt.
hend.abdelhady@nci.cu.edu.eg
Dalia
Ibrahiem
National Cancer Institute, Cairo University, Kasr Al Eini Street, Fom El Khalig, Cairo, Egypt.
dr_d_ibraheem@yahoo.com
Heba A
Nader
0000-0003-4011-5948
Genome Onco-Center, 52, Gameat Al Dowal Alarabya , Mohandeseen, Cairo, Egypt.
heba_nader_91@yahoo.com
Hossam K
Mahmoud
National Cancer Institute, Cairo University, Kasr Al Eini Street, Fom El Khalig, Cairo, Egypt.
hossamkamel06@gmail.com
Wafaa H
ElMetnawy
Faculty of Medicine, Cairo University, Al-Saray Street, El Manial, Cairo, Egypt.
metnawi@link.net
10.31557/APJCP.2020.21.3.721
Objective: IDH mutations diversely affect the prognosis of cyogenetically normal acute myeloid leukemia (CN-AML) adult patients. The aim of this study is to assess the frequency of IDH mutations and to evaluate its role in AML prognosis. Methods: We have analyzed IDH1 and 2 mutations using High Resolution Melting curve analysis (HRM) in 70 denovo AML patients. Results: The median age of AML patients is 40 years (16-75). Incidence of IDH mutations is 10/70 (14.3%); 2 (2.9%) IDH1 mutant and 8 (11.4%) IDH2 mutant. Median PB blasts of mutant IDH patients was 67.5% (25-96) vs. 44% (0-98) for wild type (p=0.065). Eight/10 (80%) mutant IDH patients had B.M blasts ≥50% vs. 2/10 (20%) <50% (p<0.001) and were classified as intermediate risk cytogenetics (p=0.020) with wild FLT3-ITD (p=0.001). Ten/10 (100%) mutant IDH patients showed wild NPM1 (p=0.049). Median OS of mutant IDH in the intermediate risk cytogenetics was 1.8 years (0.7-3.1) vs. 3.1 years (1.1-5.5) for wild IDH (p=0.05). Conclusion: IDH mutation is mainly associated with intermediate risk AML and when integrated in this specific subgroup displays a lower survival and can be considered an additional integrated molecular risk marker for AML prognosis.<br />
AML,normal karyotype,intermediate cytogenetic risk,IDH mutations,Prognosis
https://journal.waocp.org/article_89002.html
https://journal.waocp.org/article_89002_406ab715b07246c4b1e0bc7f515fe231.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
The Efficacy and Safety of Modified Docetaxel, Cisplatin, and 5-Fluorouracil Vs. Epirubicin, Oxaliplatin, and Capecitabine Regimen in the Advanced Gastric Cancer: A Randomized Controlled Clinical Trial
727
732
EN
Ahmad
Ahmadzadeh
Research Center of Thalassemia and Hemoglobinopathy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
ahmad.ahmadzadeh@gmail.com
Seyed Saeid
Seyedian
Alimentary Tract Research Center, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran.
seyedyan-s@ajums.ac.ir
Armita
Valizadeh
Department of Anatomy, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran.
valizadehg-a@ajums.ac.ir
Mahin
Soleimani
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
mahinsoleimani@yahoo.com
Pedram
Nazari
0000-0002-8598-7996
Cancer Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
dr.pedramnazari@gmail.com
Hossein
Hamidi
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
hamidi.hossein11@yahoo.com
10.31557/APJCP.2020.21.3.727
Introduction: One of the major challenges of advanced gastric cancer treatment is the lack of a standard regimen for patients. However, several clinical trials have shown that modified docetaxel, cisplatin, and 5-fluorouracil (m-DCF) and epirubicin, oxaliplatin, and capecitabine (EOX) regimens are superior to other regimens. Methods: This randomized, single-center clinical trial was performed on 40 patients with advanced gastric cancer. The first group received the m-DCF regimen as follows: docetaxel (40 mg/m2) on the first day; cisplatin (40 mg/m2) on the first and second days; and 5-fluorouracil (400 mg/m2) from the first to fourth day. The second group received the EOX regimen, including epirubicin (50 mg/m2) and oxaliplatin (130 mg/m2) i.v on the first day and capecitabine at a twice-daily dose of 625 mg/m2 p.o for 21 days. Treatment was applied every three weeks for a total of eight cycles in both groups. In each group, the overall and progression-free survival rates and toxicity were assessed. Results: A total of 40 patients were enrolled in this study (21 samples in the m-DCF group and 19 samples in the EOX group), 62.5% of whom were male. The median survival rate was 14.00 (95% CI: 11.82-16.18) months in the m-DCF group and 15.00 (95% CI: 9.56-20.43) months in the EOX group; however, differences between the groups were not significant. The progression-free survival rate was higher in the EOX group, although there was no significant difference between the two groups. Also, there was no significant difference regarding the side effects (e.g., toxicity) or need for supportive care between the groups. Conclusion: It seems that both m-DCF and EOX regimens are similar in terms of survival and toxicity and are recommended as first-line treatment for advanced gastric cancer with respect to the patient’s status.
Gastric cancer,EOX,m-DCF,Efficacy,safety
https://journal.waocp.org/article_88987.html
https://journal.waocp.org/article_88987_ffa0b53a2478568bcf69c8aebd216af7.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Effect of Metformin For Decreasing Proliferative Marker in Women with Endometrial Cancer: A Randomized Double-blind Placebo-Controlled Trial
733
741
EN
Kittisak
Petchsila
Department of Obstetrics and Gynecology, Rajavithi Hospital, Bangkok, Thailand.
boatkittisak02@gmail.com
Nisa
Prueksaritanond
Department of Obstetrics and Gynecology, Rajavithi Hospital, Bangkok, Thailand.
nisa3054@gmail.com
Putsarat
Insin
Department of Obstetrics and Gynecology, Rajavithi Hospital, Bangkok, Thailand.
mamieo3020@gmail.com
Marut
Yanaranop
Department of Obstetrics and Gynecology, Rajavithi Hospital, Bangkok, Thailand.
myanaran1@gmail.com
Nutpacha
Chotikawichean
Department of Pathology and Laboratory Medicine, Rajavithi Hospital, Bangkok, Thailand.
nu_mint7@hotmail.com
10.31557/APJCP.2020.21.3.733
Objective: To compare the Ki-67 index of endometrial cancer cells before and after treatment between the metformin and placebo group in women with endometrial cancer (EC). Methods: This study was a randomized, double-blind, placebo-controlled trial conducting in non-diabetic women who diagnosed with endometrioid EC and had a schedule for elective surgical staging at Rajavithi Hospital between August 2018 and June 2019. Tissue specimens were obtained via endometrial curettage at the time of initial diagnosis (pre-treatment) and hysterectomy (post-treatment) to assess the value of the Ki-67 index by immunochemistry. Patients were randomly assigned into 2 groups: metformin and placebo group. Metformin 850 mg or placebo 1 tab were administered once daily for at least 7 days, starting on the first morning after recruitment until one day before surgery. Baseline characteristics (e.g., age, body mass index, co-morbidities) including surgical and pathological characteristics were recorded. The metabolic effect of metformin was also evaluated by a recording of fasting blood sugar, HbA1C and potential adverse events including nausea, vomiting, dizziness, and hypoglycemic symptom. Results: A total of 49 EC patients were included in this study. Twenty-five patients were assigned to the metformin group and 24 patients were assigned to the placebo group. Baseline demographic, surgical, and pathological characteristics between the 2 groups were similar. Metformin significantly changed the Ki-67 index relative to placebo, with a mean decrease of 23.3% (p=0.001) and a mean proportional decrease of 39.1% (p=0.006) before and after treatment. Additionally, no significant differences were detected in metabolic effects and adverse events between the metformin and the placebo groups. Conclusion: Short-term treatment with an oral metformin significantly reduced a proliferative marker Ki-67 index in women with endometrioid EC awaiting surgical staging. This study supports the biological effect of metformin in EC and potential applications in the adjuvant treatment in EC patients.
Metformin,Endometrial cancer,Ki-67 index,Proliferative marker
https://journal.waocp.org/article_88974.html
https://journal.waocp.org/article_88974_3673cea99cad745ff6d8ddd8ff08073b.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Clinical Significance of CD200 and CD56 Expression in Patients with Acute Myeloid Leukemia
743
748
EN
Salah
Aref
0000-0002-4822-5204
Department of Clinical Pathology, Hematology Unit, Faculty of Medicine, Mansoura
University, Egypt.
salaharef@yahoo.com
Nashwa
Abousamra
Department of Clinical Pathology, Hematology Unit, Faculty of Medicine, Mansoura
University, Egypt.
nashwa@yahoo.com
Emann
El-Helaly
Department of Clinical Pathology, Hematology Unit, Faculty of Medicine, Mansoura
University, Egypt.
eman@yahoo.com
Mohamed
Mabed
Hematology Unit, Oncology Center, Mansoura
University, Egypt.
mabed@yahoo.com
10.31557/APJCP.2020.21.3.743
Background: Acute myeloid leukemia (AML) escape from immunosurveillance by immunosuppression. CD200 and CD56 expression represented an independent prognostic factor in many hematological malignancies but its importance in AML patients remains to be identified. Methods: CD200 and CD56 expression were assessed in the bone marrow blasts for Fifty-two (52) newly diagnosed AML by flowcytometry before start of therapy. Results: CD200+ expression was reported in 28.8% of patients while 17.3% of patients showed CD56+ expression. M4 FAB revealed high frequency of both CD200+ and CD56+ expression. The overall survival of CD200+ patients was 19.2% compared to 35.3% in CD200- (P= 0.049). On the other hand, CD56+ patients had the lowest complete remission rate (22.2% vs. 53.4%). In addition, CD56+ population had significant bad influence on overall survival than those of CD56- population (11.1 % vs. 35.5 %, P= 0.047). Conclusions: CD200 and CD56 positive expression by myeloblasts at diagnosis denote poor prognostic indicator and correlated with poor cytogenetic findings. CD200 could be used as therapeutic target in AML.<br />
CD200,CD56,AML,Prognosis
https://journal.waocp.org/article_88999.html
https://journal.waocp.org/article_88999_c57cef224bc961435e55cc573c4bdd8e.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
A Pilot Study of Extended Adjuvant Therapy with Metronomic Docetaxel for Patients with Operable Triple-Negative Breast Cancer
749
754
EN
Bader A
Abdelmaksoud
Department of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Zagazig university, Zagazig, Egypt.
babdelmaksoud@moh.gov.sa
Abdelmotaleb
Mohammed
Department of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Zagazig university, Zagazig, Egypt.
abdelmotalabmohamed@yahoo.com
Mostafa M
Toam
Department of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Zagazig university, Zagazig, Egypt.
m_toam@yahoo.com
10.31557/APJCP.2020.21.3.749
Background: Triple-negative breast(TNBC) cancer is a molecular subtype of breast cancer with poor prognosis and did not get approved targeted therapy till now. In the last years, metronomic chemotherapy (mCTH) was investigated to improve treatment outcomes in TNBC patients both in early and metastatic setting due to its anti-angiogenic and immune-stimulatory mechanisms. The aim of this study is to evaluate the efficacy and safety of extended adjuvant chemotherapy with metronomic docetaxel for patients with operable TNBC. Methods: 31 women with clinically and pathologically proved operable TNBC, either node-negative or node-positive with tumor size ≥ 0,5 cm were enrolled after finishing the primary standard of care treatment. The patients were subjected to extended adjuvant therapy for 6 months with metronomic low dose docetaxel with starting dose of 15mg/m2 in weekly bases for 4 weeks then the dose was escalated to 20 mg/m2 once per week if there were no side effects. Results: After a median follow up of 36 months (range 6-52), 24 patients (77.4%) were still alive. During the period of follow-up, 12 patients (38.7%) showed disease relapse and 19(61.3%) cases remain free of the disease. The estimated mean of DFS in our study was 38.26 months (95%CI; 31.87 – 44.65) with 2 and 3 years DFS rate of 70.5 % and 56.4% respectively while the estimated mean of OS was 43.75 months (95% CI; 38.35 – 49.16) with 2 and 3 years OS rates 83.3% and 78.1% respectively, Generally the treatment was tolerated with mild to moderate hematological and non hematological adverse events, all are grade 1,2 and treatment-related deaths were not observed. Conclusion: Extended adjuvant treatment for 6 months with metronomic docetaxel after the primary standard of care therapy was tolerated and has an encouraging survival benefit in patients with operable TNBC and these results need further evaluation in randomized control studies.<br />
metronomic chemotherapy,Docetaxel,TNBC,extended adjuvant therapy
https://journal.waocp.org/article_88976.html
https://journal.waocp.org/article_88976_8bf7bec3158d5250e62a99d4dd9fdbe0.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Correlation of Molecular Markers in High Grade Gliomas with Response to Chemo-Radiation
755
760
EN
Rohini
Khurana
0000-0002-4493-1742
Department of Radiation Oncology, Dr Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
drrohinisethi@gmail.com
Satyajeet
Rath
0000-0003-3905-4922
Department of Radiation Oncology, Dr Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
satyajeetrath@gmail.com
Harikesh Bahadur
Singh
Department of Radiation Oncology, Dr Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
harikeshsingh2005@gmail.com
Madhup
Rastogi
0000-0001-7060-7201
Department of Radiation Oncology, Dr Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
drmadhup1@gmail.com
Sambit Swarup
Nanda
0000-0002-0801-9262
Department of Radiation Oncology, Dr Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
sambit.sambitswarup@gmail.com
Abhishek
Chauhan
Department of Radiodiagnosis, Dr Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
drabhishek13@gmail.com
Mohammad
Kaif
Department of Neurosurgery, Dr Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
dr_kaifmohd@yahoo.co.in
Nuzhat
Hussain
0000-0003-1831-9063
Department of Pathology, Dr Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
drsridhar.rmlims@gmail.com
10.31557/APJCP.2020.21.3.755
Background: The standard of care in high grade glioma (HGG) is maximal safe surgical resection followed by adjuvant radiotherapy (RT) with/without chemotherapy. For anaplastic gliomas, studies have shown use of procarbazine, lomustine, vincristine (PCV) improves overall survival (OS) and progression free survival (PFS). Currently, there is substantial evidence that molecular markers strongly predict prognosis and response to treatment. Methods: Between January 2016 to January 2018, 42 patients were accrued and followed up till April 2019. The primary end points were to correlate molecular markers with response to therapy in terms of OS and PFS in HGG. The secondary end point was to evaluate frequency of 1p/19q codeletion, IDH 1 mutation, ATRX deletion and p53 in HGG patients. Results: The median age was 46 years (range 18-67) with M:F ratio 30:12. The frequency of IDH1 mutation,1p/19q codeletion, p53 mutation and ATRX mutation were 42.8%, 16.6%, 42.8% and 14.2% respectively. All the seven patients with 1p/19q codeletion had IDH1 mutation. Median follow up was 22 months. The 20-months PFS for different mutations were as follows; IDH1-mutated vs wild type: 53.6% vs 29.8%; p-0.035, 1p/19q codeleted vs non-codeleted: 85.7% vs 62.3%; p-0.011, p53 wild type vs mutated 32.1% vs 35.6%; p-0.035 and ATRX lost vs retained: 55.6% vs 53.3%; p- 0.369. The 20-months OS for IDH1 mutated vs wild type: 82.4% vs 30.6%; p-0.014, 1p/19q codeleted vs non-codeleted: 85.7% vs 65.8%; p-0.104, p53 wild-type vs mutated 45.5% vs 73.9%; p-0.036 and ATRX lost vs retained: 100% vs 60.3%; p-0.087. Conclusion: Codeletion of 1p/19q with IDH1 mutation in HGG is associated with a significantly favourable PFS. However, larger studies with longer follow up are required to evaluate OS and PFS in all the molecular subgroups.
high grade glioma,radiotherapy,1p/19q codeletion,IDH 1 mutation,Molecular markers
https://journal.waocp.org/article_88994.html
https://journal.waocp.org/article_88994_8e8e9c604e7924ac7e0533d0a5fbdbd2.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Effect of Chewing Gum on Gastrointestinal Function Recovery After Surgery of Gynecological Cancer Patients at Rajavithi Hospital: A Randomized Controlled Trial
761
770
EN
Arphamart
Nanthiphatthanachai
Department of Obstetrics and Gynecology, Rajavithi Hospital, Bangkok, Thailand.
sun_arphamart@hotmail.com
Putsarat
Insin
Department of Obstetrics and Gynecology, Rajavithi Hospital, Bangkok, Thailand.
mamieo3020@gmail.com
10.31557/APJCP.2020.21.3.761
Objective: To evaluate the efficacy of postoperative gum-chewing compare with routine postoperative care on the recovery of gastrointestinal function after comprehensive surgical staging for gynecological cancer. Materials and methods: A total of 82 patients who underwent comprehensive surgical staging for gynecological cancer at Rajavithi Hospital between October 1st, 2018 and June 30th, 2019 were randomly allocated into two groups: Gum-chewing group (n=40) and control group (n=42). In the gum-chewing group, patients were assigned to chew sugar-free gum for 30 minutes starting from the first postoperative morning then every 8 hours until the first passage of flatus. In the control group, patients have received routine postoperative care. The primary endpoint was time to first flatus after surgery. The secondary endpoints were time to first bowel sound, time to first defecation, time to first walk, postoperative analgesia and anti-emetic drug requirement, ileus symptoms, length of a hospital stay, and potential adverse events of gum-chewing, including dry mount, choking, and aspiration. Result: Chewing gum was statistically significant in reducing time to first flatus compared with routine postoperative care (median 24.7 (range 2.2-86.5) vs 35.4 (range 7.2-80.9) hours, p=0.025). The length of a hospital stay was also significantly shorter in the gum-chewing group (median 3.0 (range 1.0-8.8) vs 3.5 (range 1.8-50.0) days, p=0.023). There were no significant differences in time to first bowel sound, time to first defecation, time to first walk, postoperative analgesia and anti-emetic drug requirement, and ileus symptoms between both two groups. No adverse events related to postoperative gum-chewing were observed. Conclusion: Gum-chewing was associated with early recovery of gastrointestinal function in patients undergoing surgery for gynecological cancer. It is an inexpensive and physiologic intervention that appears to be reasonably safe and should be recommended as an adjunct in postoperative care of gynecological cancer surgery.
Gum-chewing,gynecological cancer surgery,gastrointestinal function recovery,postoperative bowel ileus
https://journal.waocp.org/article_88975.html
https://journal.waocp.org/article_88975_27f56af2d05a8a6a5be24893e219dec8.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
The Effect of Lavender Aroma on Anxiety of Patients Having Bone Marrow Biopsy
771
775
EN
Reyhaneh
Abbaszadeh
School of Nursing and Midwifery, Tehran University of Medical Sciences, Tehran, Iran.
abaszaderey04@gmail.com
Fariba
Tabari
0000-0003-0672-4977
School of Nursing and Midwifery, Tehran University of Medical Sciences, Tehran, Iran.
ftabari@tums.ac.ir
Atefeh
Asadpour
School of Nursing and Midwifery, Tehran University of Medical Sciences, Tehran, Iran.
atefeasadpur72@gmail.com
10.31557/APJCP.2020.21.3.771
Introduction: Bone marrow biopsy is a common procedure for the diagnosis and treatment of hematologic diseases and tumors, which are associated with anxiety. The purpose of this study was to examine the effect of lavender aroma on anxiety of patients having bone marrow biopsy. Materials and Methods: This study was performed on 80 patients referred to Vali-e-Asr Hospital for bone marrow biopsy. Samples were selected by convenience method and were assigned into intervention and control groups using randomized blocks of 4. Random sequence was generated by RAS software. Several drops of distilled water on a cotton ball was used in the control group and same amount of lavender essential oil on a cotton ball was used in the intervention group. Then, participants in both groups were asked to smell the cotton ball for 15 minutes and then, their anxiety level was measured immediately. The results were analyzed by SPSS software version 25 using covariance analysis and rank regression. Results: The results showed that, the mean scores of anxiety in the control and intervention groups were 6.3 ± 1.92 and 3.75 ± 1.05, respectively. There was a significant difference (p <0.05) between the two groups in terms of anxiety score.The results showed that there was a significant difference in anxiety score between two groups in terms of variables such as age, gender, physician experience, biopsy history and biopsy site (P <0.05). The results also showed no significant difference between the (p >0.05). Conclusion: The results of this study showed that bone marrow biopsy is associated with anxiety, and smelling of lavender aroma is effective in reducing anxiety in patients undergoing this procedure. This fragrance can be used by treatment team in hematology and oncology clinics to reduce anxiety caused by bone marrow biopsy.<br />
bone marrow biopsy,Anxiety,aromatherapy,Lavender
https://journal.waocp.org/article_88981.html
https://journal.waocp.org/article_88981_afdedc271a14ae7385cb4ac9594cde26.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Sustainable Prevention of Cholangiocarcinoma Through Community Participation in a High-incidence Area in Thailand
777
782
EN
Nopparat
Songserm
0000-0003-3741-367X
Department of Community Health, Faculty of Public Health, Ubon Ratchathani Rajabhat University, Ubon Ratchathani, Thailand.
nopparat.s@ubru.ac.th
Somkiattiyos
Woradet
0000-0002-5758-4081
Department of Public Health, Faculty of Health and Sports Science, Thaksin University, Phatthalung, Thailand.
somkiattiyos@tsu.ac.th
Banchop
Sripa
Department of Pathology,
Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
banchop@kku.ac.th
Akhtar
Ali
Department of Biological Science,
The University of Tulsa, Oklahoma, United State of America.
akhtar-ali@utulsa.edu
10.31557/APJCP.2020.21.3.777
Opisthorchiasis and cholangiocarcinoma (CCA) are major public health problems in Northeast Thailand, especially in Ubon Ratchathani, which are the alluvial plains. Those with poor food habits are mostly at risk of having diseases. This study was a participatory action research (PAR) aimed to define the models/plans for CCA prevention. The samples consisted of 40 community representatives. The data were collected by the PAR method. Qualitative data were analyzed by content analysis. The samples coordinately analyzed and prioritized the problems and presented information to the community. The plans consisted of (1) cultivating the right values and behaviors of eating food safe from CCA in children, (2) supervising the shops not to sell foods that are the main risk factors for CCA, (3) supporting the community leaders to act as good role models to the people, (4) assigning every housewife to cook clean and safe food from CCA, and (5) encouraging the villagers to have knowledge and awareness, which can protect them from CCA. After the community designed these plans, responsible persons were assigned to implement them. Two years later, researchers evaluated the outcome. The average scores on knowledge and attitude toward CCA were significantly higher than before implementation. For the impact of projects, prevalence of opisthorchiasis in 2016 was significantly lower than that in 2014. CCA prevention by community participation employed a combination of quantitative and qualitative studies. This study has been successful and sustainable since the community has human resources, budget, and appropriate management of the project.
Opisthorchiasis,cholangiocarcinoma,Prevention,Community participation
https://journal.waocp.org/article_88977.html
https://journal.waocp.org/article_88977_b4667a4e6ab1e8c46f346b195f587677.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Opium Use and Head and Neck Cancers: A Matched Case-Control Study in Iran
783
790
EN
Hosniyeh
Alizadeh
Neuroscience Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.
a.naghibzadeh@kmu.ac.ir
Ahmad
Naghibzadeh Tahami
Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.
naghib61@yahoo.com
Narges
Khanjani
Environmental Health Engineering Research Center, Kerman University of Medical Sciences, Kerman, Iran.
narges.khanjani@gmail.com
Vahid
Yazdi-Feyzabadi
Health Services Management Research Center, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran.
va.yazdi@gmail.com
Hadi
Eslami
Department of Otorhinolaryngology Head and Neck Surgery, Shafa Hospital, Kerman University of Medical Sciences, Kerman, Iran.
morteza.hadizade@gmail.com
Vahidreza
Borhaninejad
Social Determinants of Health Research Center, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran.
borhani777@yahoo.com
Mohammad Hasan
Larizadeh
Department of Radiation Oncology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
larizad_mh@yahoo.com
Ahmad
Enhesari
Clinical Research Unit, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran.
a.enhesari@yahoo.com
Reza
Abbasi-Rayeni
Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.
dr_abbasir2005@yahoo.com
Vahid
Moazed
0000-0003-0525-4539
Department of Hematology and Oncology, Bahonar Hospital, Kerman University of Medical Sciences, Kerman, Iran.
moazed_vahid@yahoo.com
Aliasghar
Arabi Mianroodi
Department of Otorhinolaryngology Head and Neck Surgery, Shafa Hospital, Kerman University of Medical Sciences, Kerman, Iran.
anaghibzadeh61@gmail.com
10.31557/APJCP.2020.21.3.783
Background: Head and Neck (H and N) cancers include malignant tumors of the nasal cavity, pharynx, paranasal sinuses, oral cavity, larynx and salivary glands. Opium use might be related to these cancers. The aim of this study was to investigate the relation between Opium and its Derivatives (O and D) use and the incidence of H and N cancers. Methods: In this case-control study conducted in Kerman, 140 patients with H and N cancers and 280 healthy controls (matched for age, gender, and place of residence) were included. Information about their use of O and D, cigarette smoking, alcohol and diet were collected using a structured questionnaire. Conditional logistic regression was used to investigate the relation between variables. Results: The use of opioids was associated with an increased risk of HandN cancers (Adjusted OR: 8.13; CI: 4.08-16.2). A significant dose-response relation between O and D use was observed, with high use Adjusted OR=8.91; 95% CI: 4.03-19.65 and low use Adjusted OR=6.52; 95% CI: 3.18- 13.36. This dose-response association was stronger in patients with laryngeal cancer and opioids use, with high use Adjusted OR = 11.17; 95% CI=4.48-28.09 and low use Adjusted OR = 9.46; 95% CI= 3.97- 22.52. Conclusion: The results show that opium use can be considered as an important risk factor for H and N cancers. Also in Iran, opium seems to play a more important role than cigarette smoking.<br />
Case-control,Opium,risk factor,head & neck neoplasms,Iran
https://journal.waocp.org/article_88972.html
https://journal.waocp.org/article_88972_a82b98d277837ddaf9fba6979bb2ed3c.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Evaluation of p53 and Its Target Gene Expression as Potential Biomarkers of Cholangiocarcinoma in Thai Patients
791
798
EN
Janpen
Puetkasichonpasutha
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
miyukichan.jp@gmail.com
Nisana
Namwat
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
nisana@kku.ac.th
Prakasit
Sa-Ngiamwibool
0000-0002-5090-6594
Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
prakasa@kku.ac.th
Attapol
Titapun
Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
attapillar@hotmail.com
Tuangporn
Suthiphongchai
0000-0002-0124-7780
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
tuangporn.sut@mahidol.ac.th
10.31557/APJCP.2020.21.3.791
Background: Cholangiocarcinoma (CCA), a common cancer in northeastern Thailand, is a severe disease with poor prognosis and short survival time following diagnosis. DNA damage in CCA is believed to be caused by liver fluke infection in combination with exposure to carcinogens. p53, a tumor suppressor, is the most mutated gene in human cancers including liver fluke-associated CCA. Hence, expression patterns of p53 and its target genes may be useful for diagnosis and/or prognosis of CCA patients. Methods: Differential mRNA expression of p53 and its target genes, namely, FUCA1, ICAM2 MDM2, p21, PAI-1, S100A9, and WIP1 in CCA tissue samples (n = 30) relative to matched adjacent non-tumor tissues was determined by quantitative RT-PCR and compared to clinicopathological features. Level of p53 protein was determined by immunohistochemistry and correlated with the expression of its target genes. Results: Immunohistochemistry showed elevation of p53 protein level in 77% of the cases, while RT-PCR showed downregulation of p53 mRNA and its seven target genes in 23% and 47-97% of the samples. PAI-1 was down-regulated in almost all CCA samples, thus highlighting it as a potential diagnostic marker for CCA. However, no significant clinical associations were found except for down-regulation of WIP1 that was significantly correlated with non-papillary type tissue (p-value = 0.001) and with high p53 protein level (p-value = 0.007). Conclusion: Our results demonstrated statistically significant association between down-regulation of WIP1 with non-papillary type and with high p53 protein level, and PAI-1 was down-regulated in almost all CCA. Therefore, expression level of WIP1 and PAI-1 may be useful for predicting p53 functional status and as a potential diagnostic marker of CCA, respectively.
cholangiocarcinoma,Clinicopathology,Plasminogen activator inhibitor-1,Tumor suppressor p53,Wip1
https://journal.waocp.org/article_88996.html
https://journal.waocp.org/article_88996_de0f69cda5121d66c041a872565978cc.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Cisplatin Weekly Versus Every 3 Weeks Concurrently with Radiotherapy in the Treatment of Locally Advanced Head and Neck Squamous Cell Carcinomas: What Is the Best Dosing and Schedule?
799
807
EN
Karim
Mashhour
0000-0001-9980-6474
Department of Clinical Oncology, Kasr Al-Einy Sschool of Medicine, Cairo University, Egypt.
karim.mashhour.81@gmail.com
Wedad
Hashem
Department of Clinical Oncology, Kasr Al-Einy Sschool of Medicine, Cairo University, Egypt.
weedo82@hotmail.com
10.31557/APJCP.2020.21.3.799
Purpose: The aim of this prospective randomized study is to compare cisplatin at 2 dose levels given concurrently with intensity modulated radiation therapy (IMRT) in the treatment of locally advanced HNSCC. The main objectives were to evaluate treatment toxicities, loco-regional control, tumor response and patients compliance. Methods: Patients were randomized into two groups that either received 30 mg/m2 cisplatin weekly (arm A) or 100 mg/m2 once every 3 weeks (arm B). Radiotherapy prescribed dose was 70Gy in 33 fractions. Treatment adverse events were documented. Results: Sixty patients with locally advanced HNSCC were included in this study. Recruitment started at the beginning of July 2016 and ended in July 2019. The Median follow-up was 24 months. Acute non-hematological toxicities of grade 3 or higher during the treatment course were significantly more observed in Arm B patients (76.6%) compared to Arm A patients (56.6%) with a P-value of 0.007. Hematological toxicities in the form of anemia, leucopenia and neutropenia were also significantly higher in Arm B patients with a p-value of 0.435, 0.002 & 0,002, respectively. The median 2 year loco-regional control rate in Arm B was 72.8% versus 57.6% in Arm A with a p-value of 0.015. Complete responses were similar between both groups (77%). Compliance to treatment was better in Arm A with 70% of the patients received at least 6 weekly doses where as 60% of the patients in Arm B completed the three cycles of treatment and 40 % received only 2 cycles. Conclusion: Once weekly low dose cisplatin treatment showed lower acute toxicity and a better compliance compared to once every 3 weeks high dose cisplatin treatment at the expense of a lower loco-regional control.
Cisplatin,weekly,every 3 weeks,IMRT
https://journal.waocp.org/article_88989.html
https://journal.waocp.org/article_88989_8bca64692ab0d478b6d464b7eeee7dfd.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Association of Diabetes with Oral Cancer- an Enigmatic Correlation
809
814
EN
Mounika Reddy
Mekala
Department of Oral Medicine and Radiology, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India.
mounikareddy.mekala18@gmail.com
Balaji Babu
Bangi
Department of Oral Medicine and Radiology, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India.
balajiomr2002@yahoo.co.in
Jayalatha
N
Department of Radiology, MNJ institute of oncology and regional cancer center, Hyderabad, India.
jaya.nethagani@gmail.com
Rajasekhar Reddy
lebaka
Department of Microbiology, MNJ institute of oncology and regional cancer center, Hyderabad, India.
rajasekharreddy.lebaka@gmail.com
Lakshmi Kavitha
Nadendla
Department of Oral Medicine and Radiology, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India.
drkavithanp@gmail.com
Uday
Ginjupally
Department of Oral Medicine and Radiology, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India.
uday2361@gmail.com
10.31557/APJCP.2020.21.3.809
Introduction: Association of diabetes mellitus (DM) with head and neck cancers (HNC) is still controversial. In some studies, diabetic patients had an increased risk of cancer at some HNC subsites like oral cancer, while in other studies this risk was decreased. So, the present study aims to evaluate the association of diabetes mellitus, oral cancer with and without metformin and the role of habits in association with DM and metformin in the etiology of oral cancer. Materials and methods: This study was undertaken in the Kamineni Institute of Dental Sciences in collaboration with MNJ Institute of Oncology and Regional Cancer Centre, Hyderabad. The study includes 2 main groups, they are 500 Oral cancer patients and Control group includes 500 age and gender-matched patients with habits without any oral precancerous lesion/conditions. Odds ratios (OR) and 95% confidence intervals (CI) were estimated using unconditional logistic regression. Results: Out of 1000 subjects inverse relation of DM with oral cancer was observed. On comparison between oral cancer, diabetes and habits in study group and control group, decreased risk was observed with smokers (OR: 1.131and 95%CI: 0.68 -1.86) and non-chewers (OR: 2.43 and 95% CI: 1.31 - 4.49) and non-alcoholics (OR: 1.78 and 95% C.I:1.18 - 2.68). Metformin use among diabetic participants was associated with a decreased risk of oral cancer (OR: 0.51 and 95% C.I: 0.33 - 0.77). A negative association was observed in smokers (OR: 0.19 and 95% C.I.: 0.078 - 0.459), non-chewers (OR: 0.24 and 95% C.I : 0.11- 0.53) and non-alcoholics (OR: 0.46 and 95% C.I. : 0.29 - 0.727). Conclusion: Thus the present population based study results suggest an inverse association of DM and oral cancer with metformin and negative association of habits with DM and Metformin in etiology of oral cancer.
oral cancer,Diabetes Mellitus (DM),Metformin,Tobacco,chewing tobacco and alcohol
https://journal.waocp.org/article_88990.html
https://journal.waocp.org/article_88990_9d20dbde082be376a403bb61447f18f7.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Smokeless Tobacco Products (STPs) Harbour Bacterial Populations with Potential for Oral Carcinogenicity
815
824
EN
Srivastav
Monika
Department of Oral Pathology and Microbiology, SRM Dental College, Ramapuram, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.
mailme.monikasrivastava@gmail.com
Thayalan
Dineshkumar
0000-0001-8138-9916
Department of Oral Pathology and Microbiology, SRM Dental College, Ramapuram, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.
drtdineshkumar@gmail.com
Shankaran
Priyadharini
Department of Oral Pathology and Microbiology, SRM Dental College, Ramapuram, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.
priyadharini_15@yahoo.in
Thampan
Niveditha
Department of Oral Pathology and Microbiology, SRM Dental College, Ramapuram, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.
thampan.niveditha@gmail.com
Priyadharshini
SK
Department of Oral Pathology and Microbiology, SRM Dental College, Ramapuram, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.
skpriyadharshini0903@gmail.com
Krishnan
Rajkumar
0000-0002-6875-0663
Department of Oral Pathology and Microbiology, SRM Dental College, Ramapuram, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.
drrajkumar22163@gmail.com
10.31557/APJCP.2020.21.3.815
Introduction: Smokeless Tobacco Products (STPs) vary significantly in their carcinogenicity, a feature accredited to the variation in the concentrations of carcinogenic chemicals. Tobacco associated bacteria are known to produce Tobacco-specific N-nitrosamines (TSNAs) and hence are determinants of TSNA levels in Tobacco. The primary objective of this study was to conduct a microbiological survey of STPs and to provide a baseline information of the bacterial communities present in the STPs. Materials and Methods: The present study analyzed the constituency of microbial communities in 7 different smokeless Tobacco products including four chewable (T1_CW to T4_CW), two snus (T5_Snus and T6_Snus) and one snuff sample (T7_Snuff) using high-throughput sequencing of the 16S rRNA based next generation sequencing. The Tobacco samples were also analyzed for pH and moisture content. Statistical analysis of the data obtained was done using SPSS software version 20. Pearson’s Correlation was done to analyze the correlation between pH and moisture content of the Tobacco samples. Results: A total of 11 phyla were identified in all smokeless Tobacco products. A total of 36 classes were identified across all smokeless Tobacco products and bacilli was the predominant class in all the products followed by Actinobacteria and Bacteroidia. In species level, a total of 2369 species were identified across all smokeless Tobacco products. In T1 chewable Tobacco products, predominant species was staphylococcus whereas in T2 and T3, Bacillus subtilis and pumilus were predominant. In T4 chewable Tobacco product, Virgibacillus was predominant followed by halodentrificans, staphylococcus epidermidis. In snus 1 and 2, Bacillus pumilus and subtilis were predominant. In snuff, Bacillus cereus was predominant. Snus products had the highest moisture content (15.4% and 14.3%) compared to the chewable Tobacco and snuff products. The snus products analyzed had alkaline values (pH 8.50 and 8.15) and snuff and chewable Tobacco had acidic values ranging from 5.62 to 6.09. Conclusion: The current study demonstrates that ST products differ qualitatively, quantitatively, and in their bacterial composition. There is a possibility that some of these species may contribute to oral carcinogenesis, either by influencing levels of TSNAs or directly inducing chronic inflammation.
Bacterial Population,Smokeless Tobacco Products,Oral Carcinogenicity,16S rRNA
https://journal.waocp.org/article_88982.html
https://journal.waocp.org/article_88982_d8e7ed6277c8c0213519755fd5e49b85.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Response Rate of Cisplatin Plus Docetaxel as Primary Treatment in Locally Advanced Head and Neck Carcinoma (Squamous Cell Types)
825
830
EN
Maryum
Nouman
https://orcid.org/0000-0002-2778-0743
Department of Medical Oncology, Jinnah Postgraduate Medical Center, Karachi, Pakistan.
maryum.nouman@yahoo.com
Ghulam
Haider
Department of Medical Oncology, Jinnah Postgraduate Medical Center, Karachi, Pakistan.
dr_haider1@yahoo.com
Neelma
Bukhari
0000-0002-4728-9687
Department of Medical Oncology, Jinnah Postgraduate Medical Center, Karachi, Pakistan.
neelma_bukhari@yahoo.com
Aveen
Yousuf
Department of Medical Oncology, Jinnah Postgraduate Medical Center, Karachi, Pakistan.
neeva007@hotmail.com
Rabeea
Nouman
Department of Medical Oncology, Jinnah Postgraduate Medical Center, Karachi, Pakistan.
rabeenouman@gmail.com
Mehwish Roshan
Shaikh
Department of Medical Oncology, Jinnah Postgraduate Medical Center, Karachi, Pakistan.
mahwishshaikh338@gmail.com
Shahid
Hussain
Department of Medical Oncology, Jinnah Postgraduate Medical Center, Karachi, Pakistan.
drshahidhc@gmail.com
Bhunisha
Pavan
Department of Medical Oncology, Jinnah Postgraduate Medical Center, Karachi, Pakistan.
pavan_dr@hotmail.com
Raja
Rahool
Department of Medical Oncology, Jinnah Postgraduate Medical Center, Karachi, Pakistan.
dr.rahulamani@gmail.com
Paras
Memon
Department of Medical Oncology, Jinnah Postgraduate Medical Center, Karachi, Pakistan.
dr.paras_memon@hotmail.com
Saima
Zahoor
Department of Medical Oncology, Jinnah Postgraduate Medical Center, Karachi, Pakistan.
drsaimamubbashir@gmail.com
Khalil
Mehar
Department of Medical Oncology, Jinnah Postgraduate Medical Center, Karachi, Pakistan.
dr_khalil_mehar@gmail.com
Abdus
Sami
Department of Medical Oncology, Jinnah Postgraduate Medical Center, Karachi, Pakistan.
samiabdus971@gmail.com
10.31557/APJCP.2020.21.3.825
Objective: To evaluate the response rate of Cisplatin plus Docetaxel in the treatment of locally advanced head and neck squamous cell carcinomas (HNSCC) at a tertiary care hospital in Karachi, Pakistan. Materials and Methods: It was a longitudinal study, conducted at the Department of Medical Oncology, Jinnah Postgraduate Medical Center, Karachi, Pakistan from December 2018 to June 2019. One hundred patients of age 14-66 years of age of either gender with histologically proven Squamous Cell Carcinoma of Head and Neck, Stage III and IV (locally advanced) with no distant metastases were included in the study. Patients who were declared unresectable by the otolaryngologist and those with delayed appointment for radiation were given 3 cycles of Induction Chemotherapy with Cisplatin and Docetaxel, both at a dose of 75mg/m2 3 weekly. After 3 cycles, CT scan was repeated to assess the clinical response. Those patients who had partial or complete response as per RECIST criteria were re-assessed by the otolaryngologist and were planned for surgery if disease became resectable while other patients were referred for Concurrent Chemo-Radiation Therapy (CCRT). SPSS version 23 was used to analyze data. Results: The partial response was achieved in majority of the patients after Induction Chemotherapy with Docetaxel and Cisplatin (62%) with a complete response in 12 %. However, 22% showed progression of the disease, and 4% showed stable disease. The most frequent side effects observed were diarrhea (62%) and neutropenia (57%). Conclusion: Induction chemotherapy with Cisplatin and Docetaxel is a promising regimen with good response and favorable toxicity profile and can be considered as a potentially effective outpatient regimen for locally advanced squamous cell carcinoma of head and neck.
induction chemotherapy,Squamous Cell Carcinoma,response rate,Chemotherapy,head and neck cancer
https://journal.waocp.org/article_89001.html
https://journal.waocp.org/article_89001_6c7243be902d585cfa32afbc144db00a.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Fas Ligand (FasL) in Association with Tumor-Infiltrating Lymphocytes (TILs) in Early Stage Cervical Cancer
831
835
EN
Tricia Dewi
Anggraeni
0000-0002-8876-3138
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Indonesia,
Cipto Mangunkusumo Hospital, Indonesia.
anggi73@gmail.com
Primariadewi
Rustamadji
Department of Anatomic Pathology , Faculty of Medicine, University of Indonesia, Cipto Mangunkusumo Hospital, Indonesia.
primariadewi@yahoo.com
M Farid
Aziz
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Indonesia,
Cipto Mangunkusumo Hospital, Indonesia.
mfarid_aziz@yahoo.com
10.31557/APJCP.2020.21.3.831
Objective: To date, little is known about the roles of FasL and TILs in cervical cancer. This study aims to determine the correlation between FasL expression and TILs presence in cervical cancer. Methods: In this study, we analysed the FasL and TIL presence in 32 squamous cell carcinoma or adenocarcinoma that were obtained from early stage (≤ IIA2) cervical cancer patients using immunohistochemistry. The level of FasL and TIL was assessed qualitatively, and then quantified with the H-Score system. Results: Most of the patients were between 30 to 50 years old (59,4%), and had never taken pap smear examination before (96,9%). Based on the Pearson analysis of FasL and TIL presence, we found that FasL was inversely correlated with CD45 or TIL number when the level of FasL is above 140 and the CD45 is below 160. Based on Chi-Square test of FasL and TIL classification, there was a nine-fold odds ratio (OR) of lower TILs classification in high expression of FasL classification (OR 9, p=0.01). Conclusion: An inverse correlation between FasL expression and TILs level, that might indicate FasL-induced TILs apoptosis in tumor tissue, was observed. The strong inverse correlation between FasL and TILs presence showed some insight about the interactions between cancer cells and its surroundings inside of the cervical cancer tissue. This might also be further developed to tailor a prognostic marker that can predict the outcome of therapy in patients, not only in cervical cancer, but generally in all cancer.
Cervical cancer,FASL,TIL,Apoptosis
https://journal.waocp.org/article_89004.html
https://journal.waocp.org/article_89004_e312c321a33331aa81e25c7e290a37c8.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Candida albicans Beta-Glucan Induce Anti- Cancer Activity of Mesenchymal Stem Cells against Lung Cancer Cell Line: An In-Vitro Experimental Study
837
843
EN
Fatemeh
Peymaeei
Department of Medical Mycology and Parasitology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
ava_mod@yahoo.com
Fatemeh
Sadeghi
Department of Medical Mycology and Parasitology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
tootfarang588@yahoo.com
Elahe
Safari
Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
el.safari@yahoo.com
Samaneh
Khorrami
Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
khoramis@yahoo.com
Mehraban
Falahati
Department of Medical Mycology and Parasitology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
mehrabanfalahti@yahoo.com
Shahla
Roudbar Mohammadi
Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran,Iran
sh.mohammadi@modares.ac.ir
Maryam
Roudbary
Department of Medical Mycology and Parasitology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
m_roudbary@yahoo.com
10.31557/APJCP.2020.21.3.837
Objective: β-glucan, glucopyranosyl polymers of fungi cell wall, represent an immune stimulating effects with potential anti-cancer activity. Mesenchymal stem cells (MSC) have immunomodulating properties in cancer microenvironment. The aim of this study was to investigate the anti-cancer effect of Candida albicans (C. albicans) beta-glucan on MSCs supernatant for apoptosis assay of lung cancer cells in vitro. Methods: Beta-glucan was extracted from cell wall of C.albicans. MSC isolated from adipose tissue of patients and confirmed using specific surface markers expression which examined by flow cytometry. MSCs treated with various concentrations of β-glucans for 48 hours. Cytotoxic effect of β-glucans was evaluated using MTT assay. MSC and lung cancer line cocultured and treated with β-glucans and apoptosis assay was done by flow cytometry. Results: Cytotoxicity findings showed a significant decrease in MSC viability during 48h, however it was dose-dependent (P<0.05). According to the obtained findings, supernatant of mesenchymal stem cells treated with β-glucans increased cancer cells apoptosis (P<0.05). Conclusion: Beta glucan may highlight a potential and novel promising candidate in future strategies to cause apoptosis of cancer cells and consider as therapeutic agent against tumor growth as well. Definitely, more in vitro and in vivo studies are required to understand its functions.
β-glucans,Lung cancer,Mesenchymal Stem Cells,Apoptosis
https://journal.waocp.org/article_88979.html
https://journal.waocp.org/article_88979_579bab803334d2c810677fb24ceb30c7.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Palliative Care in the Health System of Iran: A Review of the Present Status and the Future Challenges
845
851
EN
Salman
Barasteh
Medicine, Quran and Hadith Research Center, Nursing Faculty, Baqiyatallah University of Medical Sciences, Tehran, Iran.
s.barasteh@gmail.com
Maryam
Rassouli
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
rassouli.m@gmail.com
Akram
Parandeh
Medicine, Quran and Hadith Research Center, Nursing Faculty, Baqiyatallah University of Medical Sciences, Tehran, Iran.
akram.parandeh@yahoo.com
Amir
Vahedian-Azimi
Trauma Research Center, Nursing Faculty, Baqiyatallah University of Medical Sciences, Tehran, Iran.
amirvahedian63@gmail.com
Rohallah
Zaboli
Health Management Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
rouhollah.zaboli@gmail.com
Morteza
Khaghanizadeh
Medicine, Quran and Hadith Research Center, Nursing Faculty, Baqiyatallah University of Medical Sciences, Tehran, Iran.
s.barasteh@yahoo.com
10.31557/APJCP.2020.21.3.845
Objectives: In the near future, the health system of Iran will face serious public health challenges means increase in the elderly population and the rate of chronic diseases. Therefore, it is anticipated that providing palliative care for chronic diseases will be one of the priorities of the country’s health system. The purpose of the present study was to explain the present status and the future challenges of providing palliative care in the health system of Iran and help policy-makers to create a future roadmap by presenting a picture of the present status. Methods: In this qualitative study, 17 semi-structured interviews were conducted with policy-makers, researchers, and managers of the centers providing palliative care in 2018-2019. Interviews were analyzed using directed content analysis based on the Public Health Strategy and framework analysis. Results: According to the WHO Public Health Strategy, palliative care challenges categorized in 13 subcategory and four main category include policy-making, program implementation, comprehensive education and drug availability. Conclusion: Providing palliative care that is currently dispersed in some centers does not meet the needs of chronic diseases. Establishing the palliative care system as one of the major goals of the health system of Iran is possible through reforming the fourfold structure of policy-making, implementation, education and drug availability. Therefore, it is suggested that authorities perform comprehensive and systematic management of challenges using foresight methods.<br />
palliative care,health system of Iran,non-communicable disease,elderly and Public Health Model
https://journal.waocp.org/article_89006.html
https://journal.waocp.org/article_89006_69fbc81da785d6c63732f2d9bc78f5ba.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Effectiveness of a Health Education Program to Improve Knowledge and Attitude Towards Cervical Cancer and Pap Smear: A Controlled Community Trial in Malaysia
853
859
EN
Rodziah
Romli
Craniofacial and Biomaterials Sciences Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam,
Pulau Pinang, Malaysia.
rodziahromli@gmail.com
Sa’adiah
Shahabudin
0000-0001-9195-4683
Craniofacial and Biomaterials Sciences Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam,
Pulau Pinang, Malaysia.
saadiahsh@usm.my
Norkhafizah
Saddki
School of Dental Sciences, Universiti Sains Malaysia,
Health Campus, Kubang Kerian, Kelantan, Malaysia.
fizah@usm.my
Norehan
Mokhtar
Craniofacial and Biomaterials Sciences Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam,
Pulau Pinang, Malaysia.
norehanmokhtar@usm.my
10.31557/APJCP.2020.21.3.853
Objective: We examined the effectiveness of a health education program to improve; knowledge and attitude towards cervical cancer and Pap smear, and uptake of Pap smear test among female entrepreneurs in Kedah, a northern state of Malaysia. Methods: This controlled community trial involved 210 women from the districts of Alor Setar and Sungai Petani. Simple random sampling was applied to select 105 women from each district. Self-administered questionnaires were used to obtain information about the variables of interest. Health education intervention program included educational talk, demo video, experience sharing, pamphlet distribution, and text message reminders. Evaluation of outcomes was performed twice. The text message reminders acted as the cues to action that were sent between the two evaluation times at one-month interval. Women in the control group received educational talk alone. In the control group, evaluation of outcomes was done only once, which was one month after the educational talk. Results: Knowledge on cervical cancer and Pap smear, and attitude towards Pap smear among women in both intervention and control group improved significantly at Evaluation stage 1. However, no further improvements were observed in the intervention group at Evaluation stage 2. The uptake of Pap smear in the intervention group increased significantly from 48.0% at Baseline to 68.0% at Evaluation stage 1 (P<0.001), and from 68.0% to 79.0% at Evaluation stage 2 (P<0.001). A significant increase in Pap smear uptake was also seen in the control group from 63.0% at Baseline to 76.0% at Evaluation stage 1 (P=0.003). Conclusions: Educational talk alone was effective in improving knowledge on cervical cancer and Pap smear, attitude towards the test, and the actual uptake of the test. However, text reminders were more effective than having an educational talk alone in increasing uptake of Pap smear test among participants.<br />
Cervical cancer,Pap smear,Health Education,Knowledge,Attitude
https://journal.waocp.org/article_89008.html
https://journal.waocp.org/article_89008_43e4c190eccd0b671303244e1e93f758.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
21
3
2020
03
01
Epithelial Cell Adhesion Molecule mRNA Can be a Potential Marker to Predict Metastasis in Hepatocellular Carcinoma Patients
861
866
EN
Iman A
Abdelgawad
Department of Clinical Pathology, NCI, Cairo University, Cairo, Egypt.
imanaga70@yahoo.com
10.31557/APJCP.2020.21.3.861
Objectives: Epithelial cell adhesion molecule [EpCAM] is a surface marker of cancer stem cells that can maintain the capacity for malignant proliferation, invasion, metastasis, and tumor recurrence; hence its detection among hepatocellular carcinoma [HCC] patients may be an important prognostic factor. The aim of this study was to detect EpCAM mRNA expression in the whole blood of HCC patients and normal control subjects to elucidate its clinico-pathological significance among patients with HCC. Methods: This study was conducted on 74 newly diagnosed HCC patients and forty normal control subjects. Both groups were subjected to the detection of EpCAM mRNA in the whole blood using reverse transcriptase polymerase chain reaction [RT- PCR] technique. EpCAM expression was compared with some of the established prognostic factors of HCC. Results: EpCAM was detected in 17.5% of the HCC cases and was not expressed in any of the normal control subjects. EpCAM positive cases showed higher serum levels of alpha- feto protein [AFP] and carcinoembryonic antigen [CEA]. Prevalence of EpCAM positivity gave significant results with distant metastasis, lymph node metastasis, and portal vein thrombosis. Conclusion: EpCAM proved high specificity among HCC patients and its expression was associated with metastasis and portal vein thrombosis. Higher serum levels of CEA among the EpCAM positive patients may attract the attention to a subgroup of HCC patients who are more liable to develop metastasis.<br />
EpCAM,HCC,Metastasis,RT-PCR
https://journal.waocp.org/article_89003.html
https://journal.waocp.org/article_89003_ae9da9a9dabdf8371bd93b1e28545396.pdf