West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
22
S1
2021
02
01
Anticancer Activity of Natural Compounds
1
2
EN
Abdul Q
Khan
Translational Research Institute and Dermatology Institute, Academic Health System, Hamad Medical Corporation, Doha 3050,
State of Qatar.
akhan42@hamad.qa
Shahab
Uddin
0000-0003-1886-6710
Translational Research Institute and Dermatology Institute, Academic Health System, Hamad Medical Corporation, Doha 3050,
State of Qatar.
skhan34@hamad.qa
10.31557/APJCP.2021.22.S1.1
Human malignancies,Natural compound,anticancer agents
https://journal.waocp.org/article_89192.html
https://journal.waocp.org/article_89192_f6dafa591a5c1e3e542f10383fc70943.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
22
S1
2021
02
01
Andrographolide, an Antioxidant, Counteracts Paraquat- Induced Mutagenesis in Mammalian Cells
3
8
EN
Preechaya
Tajai
0000-0002-8706-4976
Faculty of Pharmacy, Payap University, Chiang Mai 50000, Thailand.
tapreechaya@gmail.com
Tawit
Suriyo
Laboratory of Pharmacology, Chulabhorn Research
Institute, Bangkok 10210, Thailand.
tawit@cri.or.th
Nuchanart
Rangkadilok
Laboratory of Pharmacology, Chulabhorn Research
Institute, Bangkok 10210, Thailand.
nuchanart@cri.or.th
Bogdan
Fedeles
Departments of Biological Engineering and Chemistry, and Center for Environmental Health Sciences,
Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
bogdan@mit.edu
John M
Essigmann
Departments of Biological Engineering and Chemistry, and Center for Environmental Health Sciences,
Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
jessig@mit.edu
Jutamaad
Satayavivad
Laboratory of Pharmacology, Chulabhorn Research
Institute, Bangkok 10210, Thailand.
jutamaad@cri.or.th
10.31557/APJCP.2021.22.S1.3
Paraquat (1,1’-dimethyl, 4,4’-bipyridinium dichloride; PQ), a commonly used herbicide worldwide, is both toxic and mutagenic. The mutagenic effect of PQ stems from its ability to redox-cycle, generating oxidative stress and subsequently oxidative DNA damage, which miscodes when replication is attempted. Andrographolide (AP1), the major constituent in the leaves of the herbaceous plant Andrographis paniculata, is a diterpenoid with reported antioxidant activity. The present study employed the mammalian cell line AS52 to investigate the protective effect of AP1 against PQ-induced mutagenesis. AP1 induced cytotoxicity in AS52 cells in a dose-dependent manner (IC50 = 15.7 µM), which allowed the selection of a non-lethal dose for the mutagenesis studies. While PQ was mutagenic in AS52 cells as evidenced by the increased levels of 6-TGr mutants, AP1 by itself did not increase the mutation frequency. However, co-treatment with AP1 (1-5 µM) or the antioxidant N-acetylcysteine (2 mM) almost completely counteracted the mutagenicity of PQ (10-100 µM) in AS52 cells. Taken together, these findings suggest that AP1, and likely by extension, A. paniculata extracts, are effective antioxidants that can protect against PQ-induced mutations, and thus could be a promising alternative treatment for PQ poisoning.
andrographolide,Antioxidant,paraquat,environmental mutagen,Carcinogen
https://journal.waocp.org/article_89193.html
https://journal.waocp.org/article_89193_cb26dd80bbb7a405b00f023580eb6cec.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
22
S1
2021
02
01
Influence of Static Magnetic Field on HeLa and Huo2 Cells in the Presence of Aloe vera Extract
9
15
EN
Mohammad
Satari
Department of Biophysics, Malayer University, Malayer, Iran.
m.satari66@yahoo.com
Fatemeh
Javani Jouni
Department of Biomedical Engineering, Faculty of Health, Tehran
Medical Sciences, Islamic Azad University, Tehran, Iran.
javani_f@yahoo.com
Parviz
Abolmaleki
Department of Biophysics, Faculty of Biological Sciences, Tarbiat
Modares University, Tehran, Iran.
parviz@modares.ac.ir
Homa
Soleimani
0000-0001-6944-2462
Department of Medical physics and physiology, School of Medicine, Arak University of
Medical Sciences, Arak, Iran.
dr.hsoleimani@arakmu.ac.ir
10.31557/APJCP.2021.22.S1.9
This research aimed to assess the impact of static magnetic field (SMF) on apoptosis rate and cell cycle progression in the presence of Aloe vera Crude Extract (ACE) in normal (Huo2) and cancer cells (HeLa). The specimens were split into one untreated group (control) and two experimental groups, including treatment with ACE (Alo) and compound treatment with SMF and ACE (Alo+SMF). MTT assay determined the IC50 value, and flow cytometry was employed to evaluate cell cycle distribution and apoptosis rates. Statistical analysis was carried out through a two-way ANOVA followed by Tukey’s post hoc test. Our results showed that combination treatment with SMF (10 mT) and ACE (Alo+SMF) significantly inhibited the cell proliferation. This increased the cell number in G2/M stage and early apoptosis in cancer cells compared to ACE treated cells after 24 and 48h but reduced the number of Huo2 cells in G2/M phase and early apoptosis after 24h. The effect of AEC on HeLa cells was intensified with increasing the SMF exposure time, such that the early apoptosis rate in Alo+SMF group had an approximate 4-fold increase compared to Alo group. This research proposes that the combination treatment accelerates the apoptosis induction of HeLa cell. During the interphase, there were significant differences between the cancer and healthy cells concerning the cell cycle. Moreover, exposure time may play an important role in the impact SMF on both healthy and cancer cells in the presence of AEC.
Magnetic field,plant extract,Apoptosis,Cell cycle,HeLa cells
https://journal.waocp.org/article_89194.html
https://journal.waocp.org/article_89194_4a8350449aced014d1327180e1867ff0.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
22
S1
2021
02
01
Xylocarpus Moluccensis Induces Cytotoxicity in Human Hepatocellular Carcinoma HepG2 Cell Line via Activation of the Extrinsic Pathway
17
24
EN
Gul-e-Saba
Chaudhry
Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Terengganu, Malaysia.
sababiochem@gmail.com
Nur Khairina Ahmed
Sohimi
Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Terengganu, Malaysia.
Habsah
Mohamad
Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Terengganu, Malaysia.
Muhammad Naveed
Zafar
Department of Chemistry,
Quaid-i-Azam University, Islamabad, 45320, Pakistan.
Aziz
Ahmed
School of Fundamental Science, Universiti Malaysia Terengganu, 21030
Kuala Nerus, Terengganu, Malaysia.
Yeong Yik
Sung
Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Terengganu, Malaysia.
Tengku Sifzizul Tengku
Muhammad
Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Terengganu, Malaysia.
10.31557/APJCP.2021.22.S1.17
Objective: Liver cancer is one of the most common causes of cancer death, with reduced survival rates. The development of new chemotherapeutic agents is essential to find effective cytotoxic drugs that give minimum side effects to the surrounding healthy tissues. The main objective of the present study was to evaluate the cytotoxic effects and mechanism of cell death induced by the crude and diethyl ether extract of Xylocarpus mouccensis on the human hepatocellular carcinoma cell line. Methods: The cytotoxicity activity was measured using the MTS assay. The mode of cell death determined by the apoptosis study, DNA fragmentation analysis done by using the TUNEL system. The pathway study or mechanism of apoptosis observed by study caspases 8, 9, 3/7 Glo-caspases method. Results: In this study, the methanol extracts prepared from leaf Xylocarpus mouccensis leaf produced cytotoxicity effect with IC50 (72hr) < 30µg/ml. The IC50 value at 72 hours exerted by diethyl ether extract of Xylocarpus moluccensis leaf was 0.22 µg/ml, which was more cytotoxic than to that of crude methanol extract. The results obtained by the colorimetric TUNEL system suggest that methanol crude extract of Xylocarpus moluccensis (leaf), diethyl ether extract of Xylocarpus moluccensis (leaf) and methanol extract of Xylocarpus granatum (bark) induced DNA fragmentation in the HepG2 cell line. Besides, the caspase-Glo assay demonstrated that diethyl ether leaf extract of Xylocarpus moluccensis triggered apoptotic cell death via activation of caspases -8, and -3/7 However, no visible activation was noticed for caspase -9. Furthermore, TLC indicates the presence of potential metabolites in an extract of Xylocarpus moluccensis. Conclusion: Thus, the present study suggests the remarkable potential of active metabolites in the extract of Xylocarpus moluccensis as a future therapeutic agent for the treatment of cancer.<br />
Apoptosis,Cytotoxicity,DNA Fragmentation,Mangroves,Xylocarpus moluccensis
https://journal.waocp.org/article_89195.html
https://journal.waocp.org/article_89195_6be04f34c401efdec3b4d60f644b7ad2.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
22
S1
2021
02
01
The Ethanol Extract of Marine Sponge Aaptos suberitoides Suppress Cell Viability, Cell Proliferation and Cell Migration in HER2-Positive Breast Cancer Cell Line
25
32
EN
Muhammad Hasan
Bashari
0000-0001-7298-0317
Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
bashari@unpad.ac.id
Fadli Zaky
Arsydinilhuda
Undergraduate Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
fadli.zaky98@gmail.com
Re Septian
Ilhamsyah
Undergraduate Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
reseptianilham@gmail.com
Annisa Dewi
Nugrahani
Undergraduate Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
annisanugrahani99@gmail.com
Ryzkianty Annis
Nurdin
Undergraduate Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
ryzkmail@gmail.com
Ajeng
Kartika
Biomedical Sciences Master Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
akuajengkartika@gmail.com
Fathul
Huda
0000-0002-4818-1862
Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
fathul@unpad.ac.id
Maman
Abdurahman
Department of Surgery, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
mamanar99@gmail.com
Tenny
Putri
Laboratory of Advanced Biomedicine, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
tenny.putri2013@gmail.com
Nurul
Qomarilla
Laboratory of Advanced Biomedicine, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
nurulqomarilla13@gmail.com
Harold
Atmaja
Laboratory of Advanced Biomedicine, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
harold.atmaja@gmail.com
Ikhwan Resmala
Sudji
Laboratory of Biomedicine, Faculty of Medicine, Universitas Andalas, Padang, Indonesia.
ir.sudji@gmail.com
Beginer
Subhan
0000-0002-2179-0721
Department of Marine Science and Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor, Indonesia.
beginer.subhan@gmail.com
Hermin Aminah
Usman
Department of Pathology Anatomy, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
hermin@unpad.ac.id
Yunisa
Pamela
Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
yunisa.pamela@unpad.ac.id
Eko Fuji
Ariyanto
Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
fuji@unpad.ac.id
Edy
Meiyanto
0000-0002-0886-6322
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.
meiyan_e@ugm.ac.id
10.31557/APJCP.2021.22.S1.25
Objective: This study aimed to investigate the cytotoxicity, anti-proliferation and anti-migration effect of the ethanol extract of Aaptos suberitoides on trastuzumab-resistant HER2+ breast cancer cell line. Methods: Aaptos suberitoides was collected from Tinjil Island, Banten, Indonesia, and was processed with maceration and ethanol extraction. HCC-1954 cells were treated with the ethanol extract and then followed by 3- [4, 5-dimethylthiazol-2-yl] -2.5 diphenyl tetrazolium bromide (MTT) assay to assess cytotoxicity, clonogenic assay and three-dimensional (3D) spheroid assay to evaluate anti-proliferative effect in two-dimensional and 3D model, respectively, and wound healing assay to determine anti-cell migration effect. Four parametric regression was used to analyse the IC50. Results: This study revealed that the ethanol extract of Aaptos suberitoides suppressed cell viability in correlation with cell death induction. The IC50 values of the ethanol extract of Aaptos suberitoides using MTT assay and clonogenic assay were 12.0 ppm and 4.36 ppm, respectively. The extract demonstrated an inhibition effect on spheroid growth. In low concentration, the extract of Aaptos suberitoides inhibited cell migration. Furthermore, MS analysis showed that the most abundant compounds in this extract has molecular weight m/z 229.81 [M+H]+. Conclusion: This study revealed that the ethanol extract of Aaptos suberitoides demonstrates cytotoxicity, anti-proliferation and anti-migration effect as well as inhibition effect on three-dimensional spheroid growth in trastuzumab-resistant HER2+ breast cancer cell line.<br />
Aaptos suberitoides,anti-cancer,HER2+ breast cancer cell line,marine sponge
https://journal.waocp.org/article_89196.html
https://journal.waocp.org/article_89196_fbe224b8d3cdbed4e14f0b285bc03713.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
22
S1
2021
02
01
Cytotoxicity of Simvastatin in Human Breast Cancer MCF-7 and MDA-MB-231 Cell Lines
33
42
EN
Andri
Rezano
0000-0003-2550-8794
Department of Biomedical Sciences, Division of Cell Biology, Faculty of Medicine Universitas Padjadjaran, Bandung, Indonesia.
andri.rezano@unpad.ac.id
Firda
Ridhayanti
0000-0002-8418-9466
Undergraduate Medical Program, Faculty of Medicine Universitas Padjadjaran, Indonesia.
firdaridhayanti.firda@gmail.com
Athaya Riski
Rangkuti
0000-0001-5727-5011
Undergraduate Medical Program, Faculty of Medicine Universitas Padjadjaran, Indonesia.
athaya16001@mail.unpad.ac.id
Taufik
Gunawan
0000-0003-0752-8855
Biomedical Sciences Master
Program, Faculty of Medicine Universitas Padjadjaran, Bandung, Indonesia.
taufik.gunawaniskandar@gmail.com
Gatot Nyarumenteng A
Winarno
0000-0001-8537-5415
Department of Obstetrics and Gynecology, Faculty
of Medicine, Padjadjaran University/Dr. Hasan Sadikin General Hospital, Bandung, Indonesia.
gatotnaw@yahoo.com
Indra
Wijaya
Department of Internal Medicine,
Faculty of Medicine Universitas Padjadjaran/Hasan Sadikin General Hospital, Bandung, Indonesia.
indrawijayaipd@gmail.com
10.31557/APJCP.2021.22.S1.33
Objective: Statins, 3-hydroxy-3-methylglutaryl co-enzyme A (HMG-CoA) reductase inhibitors, have been shown to be effective in the treatment of cardiovascular disease. Recent reports demonstrate an anticancer effect induced by statins on lung and prostate cancer cells. The present study aimed to investigate the therapeutic potential of Simvastatin can serve as chemotherapeutic agent against human breast cancer MCF-7 and MDA-MB-231 cell lines. Methods: The cytotoxic effect of simvastatin against breast cancer cells were evaluated using MTT assay. The related mechanism of cell death was further determined by trypan blue staining, morphological changes observation, and drug combination index. Results: The results showed that simvastatin treatment substantially induced cell death in a dose-dependent and time-dependent manner on MCF-7 and MDA-MB-231 cells. Simvastatin exhibited a highly cytotoxic effect on MCF7 and MDA-MB-231 with half-maximal (50%) inhibitory concentration (IC50) 8.9 μM and 4.5 μM respectively. Consistently, we observed antiproliferative effect of Simvastatin was associated with apoptosis on breast cancer cell lines by determination of morphological changes. Moreover, this drug demonstrated a synergistic activity with doxorubicin on triggering cell death in MCF7 cells, but not in MDA-MB-231. Conclusion: Simvastatin has a potent cytotoxic effect resulting in the death of human breast cancer MCF-7 and MDA-MB-231 cell lines, demonstrating its potential as a new candidate for cancer drug.<br />
Simvastatin,cell death,breast cancer,Antiproliferative,Chemosensitive
https://journal.waocp.org/article_89197.html
https://journal.waocp.org/article_89197_2ceb397865489e02f716851309e7629a.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
22
S1
2021
02
01
Trace Elements in Children with Acute Lymphoblastic Leukemia
43
47
EN
Omid Reza
Zekavat
Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
ozekavat@gmail.com
Mehran
Karimi
Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
mkarimi820@gmail.com
Fereshteh
Majidi
Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
fereshteh_2325@yahoo.com
Mohammadreza
Bordbar
Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
bordbarm@sums.ac.ir
Sezaneh
Haghpanah
Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
haghpanah@sums.ac.ir
Shirin
Parand
Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
shparand@gmail.com
Haleh
Bozorgi
0000-0001-8329-2843
Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
bozorgihaleh@yahoo.com
10.31557/APJCP.2021.22.S1.43
Objective: Although combined chemotherapy regimen leads to 80% remission in children with acute lymphocytic leukemia (ALL), malnutrition and altered serum trace elements as a consequence of chemotherapy agents, have become the new issue to deal with. With the aim to evaluate each trace element in childhood ALL, we investiguâtes six main trace elements before and after induction chemotherapy while considering age, gender and chemotherapy protocol as confounding factors. Methods: Thirty-six newly diagnosed ALL children were recruited, and trace elements were assessed by atomic absorption spectrometry technique. Trace elements (Zinc, Copper, Manganese, Magnesium, Chromium and Iron) decreased significantly after induction chemotherapy. Results: Considering the confounding factors, mean difference of elements decreased significantly, except for Chromium. Its mean difference was only significant in children younger than 10 and those who had received standard risk chemotherapy. Conclusion: In conclusion, all the studied trace elements decreased significantly after induction chemotherapy session in ALL children. This highlights the importance of complementary and supplementary management. A larger cohort study with longer follow up is warranted to elucidate the long-term effect of chemotherapy on these trace elements on the general health status, quality of life or risk of relapse in ALL children.
trace elements,Acute,Lymphoblastic leukemia,Chemotherapy
https://journal.waocp.org/article_89198.html
https://journal.waocp.org/article_89198_99fa4d9df10f2198f1eb0a417fd6d30b.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
22
S1
2021
02
01
Persian Gulf Snail Crude Venom (Conus textile): A Potential Source of Anti-Cancer Therapeutic Agents for Glioblastoma through Mitochondrial-Mediated Apoptosis
49
57
EN
Ahmad
Salimi
0000-0003-3026-6398
Department of Pharmacology and Toxicology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran.
salimikd@yahoo.com
Niloofar
Rahimitabar
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences,
Tehran, Iran.
niloofarrahimitabar@gmail.com
Amir
Vazirizadeh
Persian gulf research institute, Marine biology and Fishery sciences Department, Persian gulf University, Iran.
vaziriamir@yahoo.com
Vahed
Adhami
Department of Pharmacology and Toxicology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran.
vahed.adhami@gmail.com
Jalal
Pourahmad
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences,
Tehran, Iran.
j.pourahmadjaktaji@utoronto.ca
10.31557/APJCP.2021.22.S1.49
Background: Research on animal toxins have shown toxicity potential on cancerous cell and tissues in the cultures. Conotoxins obtained from marine cone snails show the highest toxicity potential, so that several human deaths have been attributed to this species of snail. These toxins have proven to be valuable agents to inhibit enzymes, channels and proteins, in the nervous systems of humans. Methods: We have studied the effects of Conus textile crude venom on U87MG human glioma cells and their mitochondria as main inducers of apoptosis and human embryonic kidney 293 cells (HEK293) as non-cancerous normal control cells. Cellular toxicity assessments including MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and measurement of caspase-3 activation as well as mitochondrial toxicity assays including measurement of the activity of succinate dehydrogenase (SDH) enzyme, mitochondrial swelling, reactive oxygen species (ROS) production, collapse of mitochondrial membrane potential (MMP) and cytochrome c release were performed in U87MG human glioma cells and HEK293 cells (as non-cancerous normal cells). Results: The results illustrated the significant cytotoxic effect of Conus textile crude venom on U87MG human glioma cells, that inhibits 50% (IC50=10µg/mL) of the cell growth after 12 h of exposure. Viability measurement showed which the Conus textile crude venom is selectively cytotoxic to U87MG human glioma cells, and induced activation of caspase-3 and induction of cell apoptosis via through mitochondrial signaling. Conus textile crude venom also selectively increased mitochondria swelling, ROS formation, cytochrome c release and MMP decrease in cancerous mitochondria but not normal mitochondria. Conclusion; Based on the obtained results from this investigation, it is concluded that the Conus textile crude venom contains promising natural compounds to fight U87MG human glioma cells through activation of apoptosis intrinsic pathways.
Conus Textile Crude Venom,Conotoxins,mitochondria,Apoptosis,Glioblastoma
https://journal.waocp.org/article_89199.html
https://journal.waocp.org/article_89199_ba2e7bd2e5399940a2e0acdd1829516b.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
22
S1
2021
02
01
2-Methoxy-1,4-Naphthoquinone (MNQ) Inhibits Glucose Uptake and Lactate Production in Triple-Negative Breast Cancer Cells
59
65
EN
Syukriyah Mat
Daud
Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan,
Malaysia.
syukii92@yahoo.com
Nik Soriani
Yaacob
Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan,
Malaysia.
niksoriani@usm.my
Agustine Nengsih
Fauzi
0000-0002-7636-544X
Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan,
Malaysia.
agustine@usm.my
10.31557/APJCP.2021.22.S1.59
Objective: The persistent activation of aerobic glycolysis in cancer cells results in accumulation of lactate and other metabolic intermediates that contribute to tumorigenesis. Increased glycolysis is frequently dysregulated in triple-negative breast cancer (TNBC), which promotes tumor growth and immune escape. This study was conducted to investigate the effect of 2-methoxy-1, 4-naphthoquinone (MNQ), compound extracted from Impatiens balsamina on glycolytic activities in human breast adenocarcinoma, MDA-MB-231 cells. Methods: Initially, MTT proliferation assay was used to test the cell viability with various doses of MNQ (5-100 µM). As the half maximal inhibitory concentration (IC50) was obtained, glucose uptake and lactate assays of the cells were tested with IC50 dose of MNQ. The treated cells were also subjected to gene and protein analysis of glycolysis-related molecules (GLUT1 and Akt). Results: The results showed that MNQ decreased the percentage of MDA-MB-231 cell viability in a dose-dependent manner with the IC50 value of 29 µM. The percentage of glucose uptake into the cells and lactate production decreased significantly after treatment with MNQ as compared to untreated cells. Remarkably, the expressions of GLUT1 and Akt molecules decreased in MNQ-treated cells, suggesting that the inhibition of glycolysis by MNQ is GLUT1-dependent and possibly mediated by the Akt signaling pathway. Conclusion: Our findings indicate the ability of MNQ to inhibit the glycolytic activities as well as glycolysis-related molecules in MDA-MB-231 cells, suggesting the potential of MNQ to be further developed as an effective anticancer agent against TNBC cells.
Glycolysis,GLUT1,Akt
https://journal.waocp.org/article_89200.html
https://journal.waocp.org/article_89200_53c804847333d9a07b3568798e70a5ee.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
22
S1
2021
02
01
Evaluation of Cytotoxic Effects of Methanolic Extract of Pergularia tomentosa L Growing Wild in KSA
67
72
EN
Essam Nabih
Ads
Department of Chemistry, Faculty of Science, University of Hail, Hail, Saudi Arabia.
essam.adss@yahoo.com
Amr S
Abouzied
Department of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail, Kingdom of Saudi
Arabia.
amrabou.zied@yahoo.com
Mohamed K
Alshammari
Department of Pharmaceutical Chemistry, College of Pharmacy, University of Hail, Hail, Kingdom of Saudi
Arabia.
mariam.essam2006@gmail.com
10.31557/APJCP.2021.22.S1.67
Background: Pergularia tomentosa is a member of the Apocynaceae family found in a wide geographical region including the Gulf region, North Africa and the Middle East. It is known as Fattaka, Ghalqa or Am Lebina in Saudi Arabia, It is used as a remedy for the treatment of skin sores, asthma, and bronchitis. Objective: This study aims to investigate the cytotoxic effects of methanolic extract and Latex (milky secretion) extract. Methods: The stem of Pergularia tomentosa was cut, air dried and soaked for 72 h with methanol repeatedly three times. The crude latex (milk extract) was collected from healthy stem parts of P. tomentosa L by cutting the petiole of leaves, and left to flow where a thick white liquid (Milky) were secreted, collected in amber glass tube and extracted with methanol. Further, the methanolic extract was fractionated by subsequent extraction with various solvents, viz. n-hexane, ethyl acetate and methanol. The cytotoxic effects of Pergularia tomentosa L were evaluated using three cancer cell lines of colon carcinoma (HCT-116), hepatocellular carcinoma (HepG2) and breast carcinoma (MCF-7). The cytotoxic effects of Pergularia tomentosa L extracts against HCT-116, HepG2, and MCF-7 were determined by crystal violet staining method. Results: The potency of plant extract to decrease the cell viability of human cancer cells was arranged in descending order as follows: Methanol extract (IC50 = 10.2 μg/ml, 13.6 μg/ml and 29.5μg/ml, respectively). > Milky secretion extract (IC50 = 52.6 μg/ml, 58.6 μg/ml and 120 µg/ml, respectively). Methanolic extract was strong cytotoxic activity against HCT-116 and HepG2 (IC50= 10.2, 13.6 µg/ml, respectively) and moderately activity against MCF-7 (IC50= 29.5 µg/ml). The Milky extract exhibited moderate activity against HCT-116 and HepG2 (IC50= 52.6-58.6 µg/ml, respectively) and weak activity against MCF-7 (120.0 µg/ml). Conclusion: The methanol extract of Pergularia tomentosa L showed higher cytotoxic effect as compared to the Latex (Milky secretion) extract. These extracts can be used as natural antitumor. In Future modern chromatographic separations are needed to get more quantity of metabolites. Further detailed investigation of the isolated metabolites is required to identify the phytoconstituents responsible for antioxidant and cytotoxic effects.<br />
Pergularia tomentosa L,Cytotoxicity,Antitumor,Anticancer,Column Chromatography
https://journal.waocp.org/article_89244.html
https://journal.waocp.org/article_89244_9bf3d0d00ea3cf8331b93cc8ad3a97c3.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
22
S1
2021
02
01
Selective Cytotoxicity of Kaempferia parviflora Extracts in Human Cell Lines
73
79
EN
Janpen
Tangjitjaroenkun
0000-0001-5250-0232
Faculty of Science at Si Racha, Kasetsart University, Si Racha Campus, Chonburi, Thailand.
xjanpen@yahoo.com
Waraporn
Yahayo
0000-0003-1365-0056
Faculty of Medicine, Srinakharinwirot
University, Bangkok, Thailand.
waraporn.yahayo@gmail.com
Suangson
Supabphol
0000-0002-4295-6751
Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
ssupabphol@hotmail.com
Roongtawan
Supabphol
0000-0001-8361-4611
Faculty of Medicine, Srinakharinwirot
University, Bangkok, Thailand.
roongtawans@gmail.com
10.31557/APJCP.2021.22.S1.73
Objective: Aims of this study were to (1) compare anti-proliferative activity between aqueous and ethanol Kaempferia parviflora (KP) extracts in both cancer (Human urinary bladder cancer cell, T24) and normal cell lines (Human umbilical vein endothelial cell, HUVEC). (2) confirm selective cytotoxicity of ethanol KP extract to normal and different cancer cell lines (3) investigate its cellular mechanism through p53 and SIRT1 gene expression. Methods: Phytochemical difference between aqueous and ethanol extract was determined by thin layer chromatography (TLC). Screening for cytotoxic activity in human cell lines was performed by cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent. P53 and SIRT1 gene expression were quantified using RT-PCR. Results: Results from the cell viability assay were shown as follows: (1) ethanol extract possessed higher toxicity to cancerous cells than aqueous extract (2) ethanol extract exhibited higher cytotoxicity to cancerous cells than normal cells (3) ethanol extract also showed cytotoxicity, with different levels, to three prostate cancer cell lines varying in aggressiveness. (4) ethanol KP extract induced cell death in T24 via p53 gene expression and prolonged cell survival in HUVEC through SIRT1 gene expression. Conclusion: These findings implied that ethanol KP extract might possibly be an alternative for cancer adjuvant therapy through the mechanism of selective p53 and SIRT1 gene expression.
Kaempferia parviflora,thin layer chromatography (TLC),cell survival,p53,sirt1
https://journal.waocp.org/article_89245.html
https://journal.waocp.org/article_89245_e5d1746cf155ba4b58404f9ac0bf184a.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
22
S1
2021
02
01
Anti-proliferative effects of Ziziphus spina-christi and Phlomis russeliana leaf extracts on HEK293 and MCF-7 Cell Lines and Evaluation of Bax and Bcl-2 Genes Expression Level in MCF-7 Cells
81
87
EN
Kimia
Ghaffari
0000-0002-8626-7691
Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
kimia.ghfr@gmail.com
Rahim
Ahmadi
Department of Physiology, Faculty of basic Science, Hamedan Branch, Islamic Azad University, Hamedan, Iran.
drrahahmadi@yahoo.com
Behrooz
Saberi
Department of Microbiology, Faculty of bio Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.
behrooz-saberi@yahoo.com
Pooria
Moulavi
Department of Biotechnology, Factualy of BioScineces, North Tehran Branch, Islamic Azad University, Tehran, Iran.
pooria.moulavi@gmail.com
10.31557/APJCP.2021.22.S1.81
To investigate the effects of Phlomis russeliana and Ziziphus spina-christi leaf extracts on apoptosis in breast cancer MCF-7 cells. Cell lines were divided into a control group and the groups exposed to 0.001, 0.01, 0.1, 1, and 10 mg/ml of Ziziphus spina-christi and Phlomis russeliana leaf extracts. Cell viability was quantified by the MTT assay. The expression of Bax and Bcl-2 genes was evaluated by Real-time PCR analysis. Statistical analysis was performed using ANOVA. HEK293 cell viability significantly increased in the groups exposed to 0.001, 0.01, and 0.1 mg/ml of Z.christi leaf extract and decreased in the group exposed to 10 mg/ml of P.russeliana leaf extract. MCF-7 cells viability significantly decreased in the groups exposed to 0.001, 0.01, 0.1, 1 and 10 mg/ml of Z.christi leaf extract and increased in the groups exposed to 0.001 and 0.01 mg/ml of P.russeliana leaf extract. The exposure of MCF-7 cells to 1 and 10 mg/ml of P.russeliana leaf extract also led to a significant decrease in cell viability. The cytotoxic effect of Z.christi was higher than P.russeliana leaf extracts on MCF7 cells. 1 mg/ml of Z.christi leaf extracts also significantly increased the expression level of Bax and Bcl-2 genes in MCF7 cells. Bcl-2 gene expression significantly increased in the group exposed to 10 mg/ml of P.ruseliana leaf extract.Despite P.russeliana leaf extract, lower Z.christi leaf extract concentrations inhibited MCF-7 cells proliferation. Ziziphus spina-christi and phlomis russeliana leaf extracts mechanism of action has occurred through the Bax-independent apoptotic pathway on MCF-7 cells.
breast cancer,Proliferat,Apoptosis
https://journal.waocp.org/article_89148.html
https://journal.waocp.org/article_89148_d41d8cd98f00b204e9800998ecf8427e.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
22
S1
2021
02
01
Effect of Valproic Acid on the Class I Histone Deacetylase 1, 2 and 3, Tumor Suppressor Genes p21WAF1/CIP1 and p53, and Intrinsic Mitochondrial Apoptotic Pathway, Pro- (Bax, Bak, and Bim) and anti- (Bcl-2, Bcl-xL, and Mcl-1) Apoptotic Genes Expression, Cell Viability, and Apoptosis Induction in Hepatocellular Carcinoma HepG2 Cell Line
89
95
EN
Massumeh
Sanaei
Research Center for Non-communicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran.
msnaei86@gmail.com
Fraidoon
Kavoosi
Research Center for Non-communicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran.
kavoosifraidoon@gmail.com
10.31557/APJCP.2021.22.S1.89
Backgrounds: Hepatocellular carcinoma (HCC), Primary liver cancer, is the fifth most common cancer in men. Histone deacetylation causes chromatin condensation resulting in gene silencing and tumorigenesis. These enzymes have become a novel target for the treatment of cancer. Histone deacetylase inhibitors (HDACIs) can reactivate tumor suppressor genes (TSGs) by inhibition of histone deacetylases (HDACs) activity leads to apoptosis induction in cancer cells. Further, these compounds can induce apoptosis through the intrinsic/mitochondrial pathway. Previously, we reported the effect of valproic acid (VPA) and trichostatin A (TSA) on TSGs p21WAF1/CIP1 (p21), p27Kip1 (p27), and p57Kip2 (P57) and also HDAC1 in colon cancer. The present study was designed to investigate the effect of VPA on the class I histone deacetylase (HDAC) 1, 2 and 3, TSGs p21and p53, and intrinsic mitochondrial pathway, pro- (Bax, Bak, and Bim) and anti- (Bcl-2, Bcl-xL, and Mcl-1) apoptotic genes, viability, and apoptosis in HCC HepG2 cell line. Materials and Methods: The HepG2 cells were cultured and treated with VPA. To determine viability, apoptosis, and the relative expression level of the mentioned genes, MTT assay, cell apoptosis assay, and qRT-PCR were done respectively. Results: VPA downregulated class I histone deacetylase (HDAC) 1, 2, and 3, Bcl-2, Bcl-xL, and Mcl-1 and upregulated p21, p53, Bax, Bak, and Bim resulting in apoptosis induction. Conclusion: VPA can induce apoptosis via activation of the intrinsic mitochondrial apoptotic pathway and also epigenetic reactivation of p21 and p53 through inhibition of class I HDAC 1, 2 and 3, activity.<br />
Extrinsic,Intrinsic,pathway,Apoptosis
https://journal.waocp.org/article_89454.html
https://journal.waocp.org/article_89454_90119c93b4850b8af1b0c62c2e51c98b.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
22
S1
2021
02
12
Antitumour Activities of Selected Pure Compounds Identified from the Serum of Crocodylus porosus, Malayopython reticulatus, Varanus salvator and Cuora kamaroma amboinensis
97
106
EN
Shareni
Jeyamogan
Department of Biological Sciences, School of Science and Technology, Sunway University, Bandar Sunway, Malaysia.
shareni@gmail.com
Naveed Ahmed
Khan
0000-0001-7667-8553
Department
of Basic Medical Sciences, College of Medicine, University of Sharjah, United Arab Emirates.
naveed5438@gmail.com
Ruqaiyyah
Siddiqui
College of Arts and Sciences,
American University of Sharjah, University City, Sharjah, United Arab Emirates.
rsiddiqui@aus.edu
10.31557/APJCP.2021.22.S1.97
Objectives: Here we determined antitumour effects of purified compounds such as Valdecoxib, Rofecoxib, L-Methionine and Artocarpin against cancer cell lines. Methods: Using purified compounds, assays were performed to determine their effects against cancer cell lines using growth inhibition assays, cytotoxicity assays, and cell survival assays against HeLa, PC3 and MCF7 cells. Results: The results showed that the selected small molecules L-Methionine, Rofecoxib, and Artocarpin suppressed the growth of more than 90% PC3 cells at 40µM. Similarly, Valdecoxib alone and in combination with other molecules exhibited potent growth inhibition and cytotoxicity against cancer cells tested. Peptide from the serum of M. reticulatus, demonstrated selective cytotoxicity against cancer cells without inhibiting the growth of normal cells. Conclusion: These findings are significant and provide a basis for the rational development of therapeutic anticancer agents, however intensive research is needed to determine in vivo effects of the identified molecules together with their mode of action to realize these expectations. <br />
Cytotoxicity,growth inhibition,HeLa,PC3,MCF7
https://journal.waocp.org/article_89455.html
https://journal.waocp.org/article_89455_0ca46e2ae077e28d1f015564551a64a2.pdf
West Asia Organization for Cancer Prevention (WAOCP), APOCP's West Asia Chapter.
Asian Pacific Journal of Cancer Prevention
1513-7368
2476-762X
22
S1
2021
02
01
A Comparative Study to Evaluate Efficacy of Curcumin and Aloe Vera Gel along with Oral Physiotherapy in the Management of Oral Submucous Fibrosis: A Randomized Clinical Trial
107
112
EN
Ashwini
Nerkar Rajbhoj
0000-0002-1285-9088
Department of Oral Medicine and Radiology, DY Patil Dental School, Pune, India.
dr.ashwini.nerkar@gmail.com
Tejas Mukund
Kulkarni
Department of Oral Medicine and Radiology,
SMBT Institute of Dental Science and Research, Nandi Hills, Dhamangaon, Igaturi, Nashik, India.
dr.tejas84@gmail.com
Anagha
Shete
0000-0003-4070-1963
Department of Oral Medicine
and Radiology, DY Patil Dental School, Pune, India.
dranaghashete@yahoo.com
Mrinal
Shete
0000-0003-1304-9658
Department of Oral Pathology, DY Patil Dental School, Pune, India.
shete.mrinal@gmail.com
Rucha
Gore
Department of Oral Pathology, DY Patil Dental School, Pune, India.
gorerucha03@gmail.com
Rashmi
Sapkal
0001-5158-5887
Department of Oral Medicine and Radiology, M.A.Rangoonwala College of Dental Science and Research Center , Pune, India.
drrashmisarkaar@gmail.com
10.31557/APJCP.2021.22.S1.107
Rationale (Hypothesis): The antioxidant,anti-inflammatory,immunomodulatory and anti-tumorigenic properties of natural plant’s extracts like aloe Vera and curcumin may produce beneficial therapeutic effects on OSMF patients and may lead to their symptomatic relief. Also, increase in the tissue elasticity with the help of oral physiotherapy excercises , would help in reinforcing increment in mouth opening. Aim: The aim of the study is to compare the efficacy of Curcumin gel with Aloe Vera gel when both the gel are supplemented along with oral physiotherapy in the management of OSMF. Materials and methods: A study of parallel group trial design, using simple randomization technique, was conducted on confirmed cases of OSMF. Patients were divided into two groups, one group(30 patients) was given curcumin gel(Curenext) and other group (30 patients) aloe Vera gel (Aloe Vera 100% relief) and each group was asked to do same oral physiotherapy excercises supplementally. Follow-up was done for 6 weeks and patients were assessed on the basis of improvement in mouth opening and burning sensation at 2, 4, and 6 weeks. Results: There was an improvement in both the parameters at subsequent visits, but the Aloe Vera gel showed better improvement than curcumin gel in burning sensation after 6 weeks of treatment which was highly statistically significant p < 0.01. Conclusion: Curcumin gel and Aloe Vera gel are effective in improving OSMF symptoms, but aloe Vera gel is more efficacious in burning sensation improvement without any side effects. Hence, we can advocate these drugs as adjuvant treatment in addition to the recommended treatment.
Keywords: Aloe vera,curcumin,Oral submucous fibrosis,Oral Physiotherapy
https://journal.waocp.org/article_89456.html
https://journal.waocp.org/article_89456_e9ba2b03f4af8bcd52b11ef6e773788f.pdf