TY - JOUR ID - 25442 TI - Global DNA Methylation in Precancerous and Cancerous Lesions of the Uterine Cervix JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 Y1 - 2010 PY - 2010 VL - 11 IS - 6 SP - 1741 EP - 1744 KW - Uterine cervix KW - CIN KW - cancer KW - DNA hypomethylation DO - N2 - Introduction: Persistent human papillomavirus (HPV) infection is the primary causal agent in the developmentof the uterine cervix carcinoma. Nevertheless, only a minority of high-risk HPV-associated lesions progressto cervical cancer, suggesting involvement of other molecular alterations. Among putative changes, aberrantmethylation might be a crucial event. Design: Paraffin-embedded samples of benign lesions, cervical intraepithelialneoplasia (CIN) and invasive squamous cell carcinomas (SCC) were analyzed for DNA 5-methylcytosine contentby immunohistochemistry with anti-5-methylcytosine antibodies and by high-performance liquid capillaryelectrophoresis (HPCE). Results: No significant difference of DNA 5-methylcytosine content was observed betweennormal tissues, benign lesions, low-grade lesions and high-grade lesions (p=0.6). In contrast, DNAs extractedfrom invasive SCC were hypomethylated when compared with normal and preneoplastic lesions (p=0.0004). Anassociation between global DNA hypomethylation and the SCC stage was confirmed by HPCE. Conclusions: Thetransition from CIN lesions to invasive carcinoma seems to be closely linked to global DNA hypomethylation,which could be a useful marker of invasive uterine cervical lesions. UR - https://journal.waocp.org/article_25442.html L1 - https://journal.waocp.org/article_25442_d4c852be81ac376617b57773172422c2.pdf ER -