TY - JOUR ID - 25946 TI - Detection and Genotyping of High-Risk HPV and Evaluation of Anti-Oxidant Status in Cervical Carcinoma Patients in Tamil Nadu State, India - a Case Control Study JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 Y1 - 2011 PY - 2011 VL - 12 IS - 10 SP - 2689 EP - 2695 KW - human papillomavirus KW - cervical cancer KW - HPV genotyping KW - free radicals KW - antioxidants KW - India DO - N2 - Cervical cancer is the second common type of cancer among women worldwide, with the human papillomavirus (HPV) recognized as the major causative agent. The HPV 16/18 prevalance in cervical cancer patients from the Trichy and Coimbatore districts of Tamil Nadu state, India, was evaluated in addition to an assessment of oxidative stress and antioxidant status. MDA, GSH, GPx, GST, SOD, vitamin C and vitamin E were estimated in the plasma and erythrocytes of the twenty patients and an equal number of age matched normal subjects as controls. 119 paraffin embedded tissue samples were collected to perform DNA extraction and genotyping of HPV 16/18 using specific primers. Plasma and erythrocyte TBARS level was significantly elevated in the cervical cancer patients compared to normal. It was observed that SOD, GPx, GSH levels in the erythrocyte and plasma was significantly lower in cervical cancer patients, as well as GST and Vitamins E and C levels in the plasma and catalase enzyme levels in the erythrocytes. Genotyping showed 57% positive for HPV16 and 18% for HPV18, indicating that vaccination against these two will effectively reduce the burden associated with the disease. These findings suggest possible use of antioxidant supplementation as prophylactic agents for prevention and treatment of cervical cancer. UR - https://journal.waocp.org/article_25946.html L1 - https://journal.waocp.org/article_25946_575d64b760bb6dbf150f80558d02e9b0.pdf ER -