TY - JOUR ID - 26108 TI - Antitumor Effects of Hyperthermic CO2 Pneumoperitoneum on Human Gastric Cancer Cells JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 Y1 - 2012 PY - 2012 VL - 13 IS - 1 SP - 117 EP - 122 KW - Gastric cancer KW - peritoneal carcinomatosis KW - pneunoperitoneum KW - Apoptosis KW - invasion and metastasis DO - N2 - Aim: To elucidate the effects of hyperthermic CO2 pneumoperitoneum on human gastric AGS cells. Methods:Based on a newly devised in vitro study model, we evaluated the anti-cancer effects of HT-CO2 (42-44˚C for2-4h) on human gastric cancer cells, and also the corresponding mechanisms. Results: HT-CO2 (42-44˚C for2-4h) severely inhibited cell proliferation as assessed by Cell Counting Kit-8 assay, while inducing apoptosis ina temperature- and time-dependent manner demonstrated by annexin-V/PI flow cytometry and morphologicalanalysis (Hoechst/PI fluorescence). In addition, it was found that HT-CO2 (42-44˚C for 2-4h) promoted theup-regulation of Bax by western blotting. Significantly, it could also suppress gastric cancer cell invasion andmetastasis by in vitro invasion and motility assay. Conclusion: In conclusion, HT-CO2 had an efficacious cytotoxiceffect on gastric cancer cells through Bax-induced mitochondrial apoptotic signaling. Our studies indicate thatit may serve as a potential therapy for peritoneal carcinomatosis of gastric cancer. Further investigations in vivousing animal models are now urgently needed. UR - https://journal.waocp.org/article_26108.html L1 - https://journal.waocp.org/article_26108_60b97e59bd142b3b2961221677b958a8.pdf ER -