TY - JOUR ID - 26112 TI - Apoptin Induces Apoptosis in Human Bladder Cancer EJ and BIU-87 Cells JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 Y1 - 2012 PY - 2012 VL - 13 IS - 1 SP - 135 EP - 138 KW - Apoptin KW - Urinary Bladder Neoplasms KW - Apoptosis KW - therapy DO - N2 - Objective:To investigate whether apoptin is a apoptosis-inducing protein with a potential for bladder cancertherapy. Methods: We constructed a PCDNA3/Apoptin eukaryotic expression vector, and transfected this vectorinto bladder cancer cell lines BIU-87 and EJ, then observed the results by RT-PCR, transmission electronmicroscopy, MTT assay and the flow cytometry (TUNEL method). Results: PCDNA3/Apoptin successfullyinduced a high level apoptosis in both bladder cancer cell lines, compared with the controls (p<0.05). Conclusions:Apoptin can induce high level apoptosis in human bladder cancer EJ and BIU-87 cells, which suggests a potentialfor human bladder cancer therapy. UR - https://journal.waocp.org/article_26112.html L1 - https://journal.waocp.org/article_26112_2f0543c48bd3c2ca8c55348be754f083.pdf ER -