TY - JOUR ID - 26480 TI - Mechanism of P-glycoprotein Expression in the SGC7901 Human Gastric Adenocarcinoma Cell Line Induced by Cyclooxygenase-2 JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 Y1 - 2012 PY - 2012 VL - 13 IS - 5 SP - 2379 EP - 2383 KW - COX-2 KW - P-gp KW - NF-kappa B KW - Paclitaxel KW - gastric adenocarcinoma cell DO - N2 - Objective: To investigate possible signal pathway involvement in multi-drug resistant P-glycoprotein (P-gp)expression induced by cyclooxygenase-2 (COX-2) in a human gastric adenocarcinoma cell line stimulated withpacliaxel (TAX). Methods: The effects of TAX on SGC7901 cell growth with different doses was assessed byMTT assay, along with the effects of the COX-2 selective inhibitor NS-398 and the nuclear factor-ΚB (NF-ΚB)pathway inhibitor pyrrolidine dithiocarbamate (PDTC). Influence on COX-2, NF-ΚB p65 and P-gp expressionwas determined by Western blotting. Results: TAX, NS-398 and PDTC all reduced SGC7901 growth, with dosedependence.With increasing dose of TAX, the expression of COX-2, p65 and P-gp showed rising trends, thisbeing reversed by NS-398. PDTC also caused decrease in expression of p65 and P-gp over time. Conclusion:COX-2 may induce the expression of P-gp in SGC7901 cell line via the NF-kappa B pathway with pacliaxelstimulation. UR - https://journal.waocp.org/article_26480.html L1 - https://journal.waocp.org/article_26480_e895329ba18a0ccc71fa444bcf74096d.pdf ER -