TY - JOUR ID - 27263 TI - Clinical Significance of Quantitative Analysis of Plasma Epstein- Barr Virus DNA in Patients of Xinjiang Uygur Nationality with Hodgkin’s Lymphoma JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 Y1 - 2012 PY - 2012 VL - 13 IS - 12 SP - 6379 EP - 6384 KW - Hodgkin’s lymphoma KW - Uygur nationality KW - Nested-PCR KW - Epstein-Barr virus p1asma viral load DO - N2 - Objective: To investigate the relationship between plasma EBV-DNA concentration and clinicopathologicfeatures of Hodgkin’s lymphoma cases. Methods: At first, the positive rate of plasma EBV-DNA was determinedwith a nested-PCR method using 45 specimens from Uygur HL patients, as well as 110 healthy people sampledas normal controls. Secondly, using fluorescent quantitative nested-PCR, EBV viral load was assessed in theEBV-DNA positive plasma samples. Then, relationships between plasma EBV viral load and clinicopathologicfeatures of HL patients were analyzed. Results: The positive rate of plasma EBV-DNA of HL patients wassignificantly higher than that of normal controls (53.3%vs26.4%, P=0.001). There was no significant differenceabout plasma EBV viral load between EBV–associated HL and EBV-DNA positive normal people (P=0.490).Looking at patients’ characteristics, plasma EBV viral load in 10-20 years EBV–associated HL was higher than inEBV cases which were less than 10 years or more than 35 years (P=0.025). Furthermore, in EBV-associated HL,concentration of plasma EBV-DNA was significantly higher in advanced stage disease (stages Ⅲ–IV; P=0.013),and with B-symptoms (P=0.020). Conclusion: EBV-DNA levels were associated with part of clinicopathologicfeatures of cases. It was of practical use to screen HL. Further etiological studies appear warranted. UR - https://journal.waocp.org/article_27263.html L1 - https://journal.waocp.org/article_27263_d8c23227037fe85f525e3d8e0b988b71.pdf ER -