TY - JOUR ID - 27355 TI - Construction and Expression of an Eukaryotic Expression Vector Containing the IER3 Gene JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 Y1 - 2013 PY - 2013 VL - 14 IS - 1 SP - 507 EP - 510 KW - IER3 gene KW - eukaryotic expression vector KW - Tumor DO - N2 - Background: More and more research indicate that the immediately early response gene 3 (IER3) is involvedinmany biological provesses, such as apoptosis and immunoreaction, as well as viral infection, tumorigenesisand tumour progression. Methods: Here we describe the construction of an eukaryotic expression vectorcontaining IER3 gene and its expression in A549 cells as assessed through fluorescence microscopyand Westernblotting.Results: Fluorescence detection displayed that GFP in cytoplasm was high during 48 and 72 hourspost-transfection. In addition, Western blotting showed significant increase in IER3 gene expression in thetransfected cells compared with controls. Conclusion: The recombinate plasmid expression vector was constructedsuccessfully, which may provide a basis for further exploration of function of IER3 in lung cancer. UR - https://journal.waocp.org/article_27355.html L1 - https://journal.waocp.org/article_27355_2d3a4e48045ede89e707418f9e3861ba.pdf ER -