TY - JOUR ID - 28280 TI - Effects of PTTG Down-regulation on Proliferation and Metastasis of the SCL-1 Cutaneous Squamous Cell Carcinoma Cell Line JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 Y1 - 2013 PY - 2013 VL - 14 IS - 11 SP - 6245 EP - 6248 KW - Cutaneous Squamous Cell Carcinoma KW - pituitary tumor KW - transforming gene KW - Proliferation KW - Metastasis DO - N2 - Aims: To study effects of down-regulation of pituitary tumor-transforming gene (PTTG) on proliferationand metastasis ability of the SCL-1 cutaneous squamous cell carcinoma (CSCC) cell line and explore relatedmechanisms. Methods: SCL-1 cells were divided into 3 groups (untreated, siRNA control and PTTG siRNA). Cellproliferation assays were performed using a CCK-8 kit and proliferation and metastasis ability were analyzedusing Boyden chambers. In addition, expression of MMP-2 and MMP-9 was detected by r-time qPCR andWestern blotting. Results: Down-regulation of PTTG could markedly inhibit cell proliferation in SCL-1 cells,compared to untreated and control siRNA groups (P < 0.05). Real-time qPCR demonstrated that expressionlevels of PTTG, MMP-2 and MMP-9 in the PTTG siRNA group were 0.8%, 23.2% and 21.3% of untreatedlevels. Western blotting revealed that expression of PTTG, MMP-2 and MMP-9 proteins in the PTTG siRNAgroup was obviously down-regulated. The numbers of migrating cells (51.38±4.71) in the PTTG siRNA groupwas obviously lower than that in untreated group (131.33±6.12) and the control siRNA group (127.72±5.20) (P< 0.05), suggesting that decrease of proliferation and metastasis ability mediated by PTTG knock-down maybe closely correlated with down-regulation of MMP-2 and MMP-9 expression. Conclusion: Inhibition of PTTGexpression may be a new target for therapy of CSCC. UR - https://journal.waocp.org/article_28280.html L1 - https://journal.waocp.org/article_28280_c624a26d7e83438c9cc79c2a6f2394d5.pdf ER -