TY - JOUR ID - 28489 TI - Serum miR-19a Predicts Resistance to FOLFOX Chemotherapy in Advanced Colorectal Cancer Cases JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 Y1 - 2013 PY - 2013 VL - 14 IS - 12 SP - 7421 EP - 7426 KW - advanced colorectal cancer KW - miRNA KW - FOLFOX resistance KW - serum biomarker DO - N2 - Background: Colorectal cancer is the fourth most common cancer worldwide and the second leading causeof cancer-related death. FOLFOX is the most common regimen used in the first-line chemotherapy in advancedcolorectal cancer, but only half of the patients respond to this regimen and we have almost no clue in predictingresistance in such first-line application. Methods: To explore the potential molecular biomarkers predicting theresistance of FOLFOX regimen as the first-line treatment in advanced colorectal cancer, we screened microRNAsin serum samples from drug-responsive and drug-resistant patients by microarrays. Then differential microRNAexpression was further validated in an independent population by reverse transcription and quantitative realtimePCR. Results: 62 microRNAs expressing differentially with fold-change >2 were screened out by microarrayanalysis. Among them, 5 (miR-221, miR-222, miR-122, miR-19a, miR-144) were chosen for further validationin an independent population (N=72). Our results indicated serum miR-19a to be significantly up-regulated inresistance-phase serum (p=0.009). The ROC curve analysis showed that the sensitivity of serum miR-19a todiscriminate the resistant patients from the response ones was 66.7%, and the specificity was 63.9% when theAUC was 0.679. We additionally observed serum miR-19a had a complementary value for cancer embryonicantigen (CEA). Stratified analysis further revealed that serum miR-19a predicted both intrinsic and acquireddrug resistance. Conclusions: Our findings confirmed aberrant expression of serum miR-19a in FOLFOXchemotherapy resistance patients, suggesting serum miR-19a could be a potential molecular biomarker forpredicting and monitoring resistance to first-line FOLFOX chemotherapy regimens in advanced colorectalcancer patients. UR - https://journal.waocp.org/article_28489.html L1 - https://journal.waocp.org/article_28489_5343f96afbab502bea37ab2c4496efa5.pdf ER -