TY - JOUR ID - 28891 TI - Alternative Messenger RNA Splicing of Autophagic Gene Beclin 1 in Human B-cell Acute Lymphoblastic Leukemia Cells JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 Y1 - 2014 PY - 2014 VL - 15 IS - 5 SP - 2153 EP - 2158 KW - Beclin 1 KW - alternative splicing KW - Autophagy KW - Acute leukemia DO - N2 - Beclin 1 is a key factor for initiation and regulation of autophagy, which is a cellular catabolic processinvolved in tumorigenesis. To investigate the role of alternative splicing of Beclin1 in the regulation of autophagyin leukemia cells, Beclin1 mRNA from 6 different types of cell lines and peripheral blood mononuclear cellsfrom 2 healthy volunteers was reversely transcribed, subcloned, and screened for alternative splicing. Newtranscript variants were analyzed by DNA sequencing. A transcript variant of Beclin 1 gene carrying a deletionof exon 11, which encoded a C-terminal truncation of Beclin 1 isoform, was found. The alternative isoform wasassessed by bioinformatics, immunoblotting and subcellular localization. The results showed that this variabletranscript is generated by alternative 3’ splicing, and its translational product displayed a reduced activity ininduction of autophagy by starvation, indicating that the spliced isoform might function as a dominant negativemodulator of autophagy. Our findings suggest that the alternative splicing of Beclin 1 might play important rolesin leukemogenesis regulated by autophagy. UR - https://journal.waocp.org/article_28891.html L1 - https://journal.waocp.org/article_28891_62bd15d279d11b7c86f620fe2eb08ba4.pdf ER -