TY - JOUR ID - 29318 TI - miR-200a Inhibits Tumor Proliferation by Targeting AP-2γ in Neuroblastoma Cells JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 Y1 - 2014 PY - 2014 VL - 15 IS - 11 SP - 4671 EP - 4676 KW - miR-200a KW - AP-2γ KW - neuroblastoma KW - Cell proliferation DO - N2 - Background: MicroRNA-200a (miR-200a) has been reported to regulate tumour progression in severaltumours but little is known about its role in neuroblastoma. Our aim was to investigate the potential role andmechanism of miR-200a in neuroblastomas. Materials and Methods: Expression levels of miR-200a in tissueswere determined using RT-PCR. The effect of miR-200a and shAP-2γ on cell viability was evaluated using MTSassays, and target protein expression was determined using Western blotting and RT-PCR. Luciferase reporterplasmids were constructed to confirm direct targeting. Results were reported as mean±S.E.M and differenceswere tested for significance using the 2-tailed Students t-test. Results: We determined that miR-200a expressionwas significantly lower in neuroblastoma tumors than the adjacent non-cancer tissue. Over-expression of miR-200are reduced cell viability in neuroblastoma cells and inhibited tumor growth in mouse xenografts. We identifiedAP-2γ as a novel target for miR-200a in neuroblastoma cells. Thus miR-200a targets the 3’UTR of AP-2γ andinhibits its mRNA and protein expression. Furthermore, our result showed that shRNA knockdown of AP-2γin neuroblastoma cells results in significant inhibit of cell proliferation and tumor growth in vitro, supportingan oncogenic role of AP-2γ in neuroblastoma. Conclusions: Our study revealed that miR-200a is a candidatetumor suppressor in neuroblastoma, through direct targeting of AP-2γ. These findings re-enforce the proposalof AP-2γ as a therapeutic target in neuroblastoma. UR - https://journal.waocp.org/article_29318.html L1 - https://journal.waocp.org/article_29318_58a9636938b9cddef4904d3cb3886ca8.pdf ER -