TY - JOUR ID - 30070 TI - Detection of PIK3CA Gene Mutations with HRM Analysis and Association with IGFBP-5 Expression Levels in Breast Cancer JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 Y1 - 2014 PY - 2014 VL - 15 IS - 21 SP - 9327 EP - 9333 KW - PIK3CA KW - PI3K KW - IGFBP5 KW - HRM assay KW - Turkish breast cancer KW - Mutation DO - N2 - Breast cancer is the second most common cancer and second leading cause of cancer deaths inwomen. Phosphatidylinositol-3-kinase (PI3K)/AKT pathway mutations are associated with cancer andphosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) gene mutations have beenobserved in 25-45% of breast cancer samples. Insulin growth factor binding protein-5 (IGFBP-5) can showdifferent effects on apoptosis, cell motility and survival in breast cancer. We here aimed to determine theassociation between PIK3CA gene mutations and IGFBP-5 expressions for the first time in breast cancerpatients. Frozen tumor samples from 101 Turkish breast cancer patients were analyzed with high resolutionmelting (HRM) for PIK3CA mutations (exon 9 and exon 20) and 37 HRM positive tumor samples were analyzedby DNA sequencing, mutations being found in 31. PIK3CA exon 9 mutations (Q546R, E542Q, E545K, E542Kand E545D) were found in 10 tumor samples, exon 20 mutations (H1047L, H1047R, T1025T and G1049R)in 21, where only 1 tumor sample had two exon 20 mutations (T1025T and H1047R). Moreover, we detectedone sample with both exon 9 (E542Q) and exon 20 (H1047R) mutations. 35% of the tumor samples with highIGFBP-5 mRNA expression and 29.4% of the tumor samples with low IGFBP-5 mRNA expression had PIK3CAmutations (p=0.9924). This is the first study of PIK3CA mutation screening results in Turkish breast cancerpopulation using HRM analysis. This approach appears to be a very effective and reliable screening method forthe PIK3CA exon 9 and 20 mutation detection. Further analysis with a greater number of samples is needed toclarify association between PIK3CA gene mutations and IGFBP-5 mRNA expression, and also clinical outcomein breast cancer patients. UR - https://journal.waocp.org/article_30070.html L1 - https://journal.waocp.org/article_30070_3c8d06d5430de59f06a7a0ae8de68b05.pdf ER -