TY - JOUR ID - 30084 TI - Apoptotic Effects of Eugenol-loaded Nanoemulsions in Human Colon and Liver Cancer Cell Lines JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 Y1 - 2014 PY - 2014 VL - 15 IS - 21 SP - 9159 EP - 9164 KW - reactive oxygen species KW - Liver KW - colon cell line KW - Eugenol KW - eugenol nanoemulsion DO - N2 - Background: In this study eugenol (EU) loaded nanoemulsions (NEs) emulsified with modified starch wereprepared and their apoptotic potential against liver and colon cancer cells was examined in comparison withbulk EU. Materials and Methods: We prepared stable EU loaded NEs whcih were characterized by dynamiclight scattering, centrifugation and gas chromatography. Furthermore, cell viability was determined using MTTassay, and apoptosis and cell cycle analysess by flow cytometry. Results: HB8065 (liver) and HTB37 (colon) cellswhen treated with EU:CA NEs demonstrated greater apoptotic cells percentages as evidenced by microscopicimages and flow cytometric evaluations. It was observed that EU and EU:CA NE induced apoptosis in both celllines via reactive oxygen species (ROS) generation. Conclusions: The present study demonstrated that ROSplays a critical role in EU and EU:CA NE induced apoptosis in HB8065 and HTB37 cells. This is the first reporton the antiproliferative mechanisms of EU loaded NE. UR - https://journal.waocp.org/article_30084.html L1 - https://journal.waocp.org/article_30084_668c1e69773b96f3eef7a8e09033332b.pdf ER -