TY - JOUR ID - 30662 TI - Hep88 mAb-Mediated Paraptosis-Like Apoptosis in HepG2 Cells via Downstream Upregulation and Activation of Caspase-3, Caspase-8 and Caspase-9 JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 Y1 - 2015 PY - 2015 VL - 16 IS - 5 SP - 1771 EP - 1779 KW - Hepatocellular carcinoma KW - Hep88 monoclonal antibody KW - paraptosis KW - caspase DO - N2 - Hepatocellular carcinoma (HCC) is a leading cause of cancer death worldwide. Presently, targeted therapyvia monoclonal antibodies to specific tumor-associated antigens is being continuously developed. Hep88 mAb hasproven to exert tumoricidal effects on the HepG2 cell via a paraptosis-like morphology. To verify the pathway, wethen demonstrated downstream up-regulation of caspase-3, caspase-8 and caspase-9, assessingmRNA expressionby real-time PCR and associated enzyme activity by colorimetric assay. Active caspase-3 determination wasalso accomplished by flow cytometry. Active caspase-3 expression was increased by Hep88 mAb treatment in adose-and time-dependent manner. All of the results indicated that Hep88 mAb induced programmed cell deathin the HepG2 cell line from paraptosis-like to apoptosis by downstream induction of caspases. These conclusionsimply that Hep88mAb might be a promising tool for the effective treatment of HCC in the future. UR - https://journal.waocp.org/article_30662.html L1 - https://journal.waocp.org/article_30662_14c7c3e6609d835860401827f242f455.pdf ER -