TY - JOUR ID - 30851 TI - Prognostic Relevance of Human Telomerase Reverse Transcriptase (hTERT) Expression in Patients with Gall Bladder Disease and Carcinoma JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 Y1 - 2015 PY - 2015 VL - 16 IS - 7 SP - 2923 EP - 2928 KW - Gall bladder carcinoma KW - cholelithiasis KW - Cholecystitis KW - human telomerase reverse transcriptase KW - PCR DO - N2 - Background: Gallbladder carcinoma (GBC) has been stated as an Indian disease, with the highest numberof cases being reported from certain districts of northeast India, which has an ethnically distinct population.Unfortunately there are no scientific reports on the underlying molecular mechanisms associated with thepathogenesis of the disease from this region. Aim: The present study evaluated the role of differential expressionof human telomerase reverse transcriptase (hTERT) in the development of gall bladder anomalies. Materialsand Methods: Blood and tissue samples were collected from patients undergoing routine surgical resection forclinically proven cases of gallbladder disease {cholelithiasis (CL, n=50), cholecystitis (CS, n=40) and GBC (n=30)along with adjacent histopathologically proved non-neoplastic controls (n=15)} with informed consent. Wholeblood was also collected from age and sex matched healthy controls (n=25) for comparative analysis. DifferentialhTERT mRNA expression was evaluated by semi-quantitative rt-PCR and real-time PCR based analysis usingβ-actin as an internal control. Evaluation of differential hTERT protein expression was studied by Western blotanalysis and immunoflourescence. Statistical analysis for differential expression and co-relation was performedby SPSSv13.0 software. Results: Gallbladder anomalies were mostly prevalent in females. The hTERT mRNAand protein expression increased gradiently from normal