TY - JOUR ID - 33071 TI - MiR-675 Promotes the Growth of Hepatocellular Carcinoma Cells Through Cdc25A Pathway JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Yu, Ya-Qun AU - Weng, Jun AU - Li, Shu-Qun AU - Li, Bo AU - Lv, Jun AD - Department of Hepatobiliary Surgery, the Affiliated Hospital of Guilin Medical College, Guilin, China AD - Y1 - 2016 PY - 2016 VL - 17 IS - 8 SP - 3881 EP - 3885 DO - N2 -   Background: MicroRNAs (miRNAs) have fundamental roles in tumorigenesis. MiR-675 is upregulated in hepatocellular carcinoma(HCC) cells. However, the roles of miR-675 in hepatocellular carcinogenesis are still not fully elucidated. In this study, we focus on investigating the effect and mechanism of miR-675 in proliferation of HCC cells. Materials and Methods: The cell proliferation was measured by MTT assays after transfection with miR-675 inhibitor and miR-675 mimics in HCC cells. The expression level of miR-675 was detected by real-time quantitative reverse transcription polymerase chain reaction. Protein expression of Cdc25A was measured by western blotting analysis. Results: In MTT assays, overexpression of miR-675 promoted the proliferation of HCC cells(PPPPp<0.05) after treatment with miR-675 mimics. The expression level of Cdc25A was significantly decreased (p<0.05) after treatment with miR-675 inhibitor. Conclusions: Our results indicate that miR-675 promotes the proliferation in human hepatocellular carcinoma cells by associating with Cdc25A signaling pathway. UR - https://journal.waocp.org/article_33071.html L1 - https://journal.waocp.org/article_33071_2f5a13c3e41fe1dfaca7cc2687e67c83.pdf ER -