TY - JOUR ID - 50406 TI - Potential Diagnostic and Prognostic Value of Lymphocytic Mitochondrial DNA Deletion in Relation to Folic Acid Status in HCV-Related Hepatocellular Carcinoma JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - N Zekri, Abdel-Rhaman AU - Salama, Hosny AU - Medhat, Eman AU - Hamdy, Sherif AU - Hassan, Zeinab K AU - Bakr, Yasser Mabrouk AU - Youssef, Amira Salah El-Din AU - Saleh, Doaa AU - Saeed, Ramy AU - Omran, Dalia AD - Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, Egypt. AD - Endemic Medicine Department, Faculty of Medicine, Cairo University, Cairo, Egypt. AD - Department of public health, Faculty of Medicine, Cairo University, Cairo, Egypt. AD - Yassin Abdel Ghaffar Charity Hospital, Cairo University, Cairo, Egypt. Y1 - 2017 PY - 2017 VL - 18 IS - 9 SP - 2451 EP - 2457 KW - HCC KW - mitochondrial DNA deletions KW - serum folic acid KW - Diagnosis KW - Prognosis DO - 10.22034/APJCP.2017.18.9.2451 N2 -   Objective: We assessed the possibility of using mitochondrial (mt) DNA deletion as a molecular biomarker for disease progression in HCV-related hepatocellular carcinoma (HCC) and to identify its association with folic acid status. Methods: Serum folic acid and lymphocytic mtDNA deletions were assessed in 90 patients; 50 with HCC, 20 with liver cirrhosis (LC), and 20 with chronic hepatitis C (CHC) compared to 10 healthy control subjects. The diagnostic accuracy of mtDNA deletions frequency was evaluated using receiver-operating characteristic (ROC) curve analysis Survival analysis was performed using the Kaplan-Meier method. Differences in the survival rates were compared using log-rank test. Result: Our data revealed a significant elevation of mtDNA deletions frequency in the HCC group compared to the other groups (P-value <0.01). Also, our data showed a significant correlation between folate deficiency and high frequency of mtDNA deletions in patients with HCV-related HCC when compared to the other groups (r= -0.094 and P-value <0.05). Moreover, the size of the hepatic focal lesion in the HCC patients was positively correlated with mtDNA deletions (r= 0.09 and P-value <0.01). The median survival time for the HCC patients with high frequency of mtDNA deletions (ΔCt ≥3.9; 5.7+ 0.6 months) was significantly shorter than those with low mtDNA deletions frequency (ΔCt < 3.9; 11.9+ 0.04 months, P-value <0.01). Conclusion: Our data provided an evidence that lymphocytic mtDNA deletion could be used as non-invasive biomarker for disease progression and patients’ survival in HCV-related HCC. Also, our findings implied a causal relationship between the folate deficiency and the high mtDNA deletions frequency among Egyptian patients with HCV related HCC. UR - https://journal.waocp.org/article_50406.html L1 - https://journal.waocp.org/article_50406_6152aace09e84aa662c42bcbdcbaf3ec.pdf ER -