TY - JOUR ID - 57589 TI - ADH1B, ALDH2, GSTM1 and GSTT1 Gene Polymorphic Frequencies among Alcoholics and Controls in the Arcadian Population of Central India JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Mansoori, Abdul Anvesh AU - Jain, Subodh Kumar AD - Department of Biotechnology, Dr. Harisingh Gour University, Sagar 470 003 M.P. India. Y1 - 2018 PY - 2018 VL - 19 IS - 3 SP - 725 EP - 731 KW - Keywords:- Alcoholic KW - Xenobiotic machinery KW - gene polymorphism KW - reactive oxygen species DO - 10.22034/APJCP.2018.19.3.725 N2 - Background: Epidemiological research has highlighted the global burden of primary liver cancer cases due toalcohol consumption, even in a low consumption country like India. Alcohol detoxification is governed by ADH1B,ALDH2, GSTM1 and GSTT1 genes that encode functional enzymes which are coordinated with each other to removehighly toxic metabolites i.e. acetaldehyde as well as reactive oxygen species generated through detoxification processes.Some communities in the population appears to be at greater risk for development of the liver cancer due to geneticpredispositions. Methods: The aim of this study was to screen the arcadian population of central India in order toinvestigate and compare the genotype distribution and allele frequencies of alcohol metabolizing genes (ADH1B,ALDH2, GSTM1 and GSTT1) in both alcoholic (N=121) and control (N=145) healthy subjects. The gene polymorphismanalysis was conducted using PCR and RFLP methods. Results: The allele frequency of ALDH2 *1 was 0.79 and ofALDH2*2 was 0.21 (OR:1.12; CI (95%): 0.74-1.71). The null allele frequency for GSTM1 was 0.28 (OR:0.85; CI(95%): 0.50-1.46) and for GSTT1 was 0.20 (OR:1.93; CI (95%): 1.05-3.55). No gene polymorphism for ADH1B wasnot observed. The total prevalence of polymorphisms was 3.38% for ALDH2, GSTM1 and GSTT1. Conclusion: Theresults of this study suggested that individuals of the Central India population under study are at risk for liver disordersdue to ALDH2, GSTM1 and GSTT1 gene polymorphisms. This results may have significance for prevention of alcoholdependence, alcoholic liver disorders and the likelihood of liver cancer. UR - https://journal.waocp.org/article_57589.html L1 - https://journal.waocp.org/article_57589_57d7e16baab190f385e2ebe57b996c48.pdf ER -