TY - JOUR ID - 58018 TI - Synergistic Growth Inhibitory Effects of Chrysin and Metformin Combination on Breast Cancer Cells through hTERT and Cyclin D1 Suppression JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Rasooli, Sara AU - Zarghami, Nosratollah AD - Department of Genetics, Faculty of Sciences, Islamic Azad University, Tabriz Branch, Tabriz, Iran. AD - Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Y1 - 2018 PY - 2018 VL - 19 IS - 4 SP - 977 EP - 982 KW - breast cancer KW - Cyclin D1 KW - hTERT KW - Metformin KW - silibinin DO - 10.22034/APJCP.2018.19.4.977 N2 - Objective: To explore the possibility of a novel chemopreventive strategy for improving breast cancer treatment,the anticancer effects of a combination two natural compounds, Chrysin and Metformin, against T47D breast cancercells were investigated. Materials and Methods: After treatment of T47D cells with Metformin, Chrysin and the twodrugs in combination, toxicity to cancer cells was evaluated by MTT assay. Real time PCR was then used to determinethe expression levels of hTERT and cyclin D1 genes. Results: The MTT test findings showed that the combination ofmetformin and chrysin had high synergistic effects in killing cancer cells. In addition PCR demonstrated a significantdecrease in cyclin D1 and hTERT gene expression in the T47D breast cancer cell line. Conclusion: The conmbinationof metformin and chrysin suppressing hTERT and cyclin D1 gene expression might offer an appropriate approach forbreast cancer therapy. UR - https://journal.waocp.org/article_58018.html L1 - https://journal.waocp.org/article_58018_84338d012ec11e848ac9e5a36b0e6c10.pdf ER -