TY - JOUR ID - 66112 TI - Derivative of Stevioside; CPUK02; Restores ESR1 Gene Methylation in MDA-MB 231 JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Khazayel, Saeed AU - Mokarram, Pooneh AU - Mohammadi, Zeinab AU - Ramezani, Fatemeh AU - Dayong, Zhang AD - Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. AD - Vice Director of Lianyungang Eco. & Tech. Development Zone Management Committee and Drug Research Institute, China Pharmaceutical University, China. Y1 - 2018 PY - 2018 VL - 19 IS - 8 SP - 2117 EP - 2123 KW - CPUK02 KW - DNMT KW - Gene expression KW - Methylation pattern KW - Estrogen Receptor DO - 10.22034/APJCP.2018.19.8.2117 N2 - Background: CPUK02 (15-Oxosteviol benzyl ester) is a new ent-kaurenoid derivative of stevioside and exhibitsstrong anti-cancer activity. Nowadays, the pattern of epigenetic in cancer has been topic of many studies and DNAmethylation targeting represents a relevant strategy for cancer treatment. Since, no study conducted to this mechanism, weattempt to evaluate whether CPUK02 induce its anti-cancer effects via alteration the level of mRNA DNMT3B, DNMT3Aexpression and ESR1 methylation pattern in breast cancer cells line. Methods: MCF-7 (ER +) and MDA-MB231 (ER-)cell lines were treated for 24, 48 hours with 1 μM CPUK02 and 5-AZA-CdR (DNA methyltransferase inhibitor).Quantitative expression of DNMT3B and DNMT3A genes and ESR1 promoter methylation was assessed by Real-TimePCR and MS-PCR, respectively. Results: CPUK02 restored ESR1 promoter unmethylated allele in MDA-MB 231cells. Also treatment with CPUK02 decreased the expression of both DNMT3A and DNMT3B genes like 5-AZA.The expression of DNMT genes were diminished by half compared with control cells. Conclusions: These results showedthat CPUK02 has an anticancer effect on MDA-MB 231 cells which this effect can be done through several pathways. UR - https://journal.waocp.org/article_66112.html L1 - https://journal.waocp.org/article_66112_cc0f0c9916ea739318c09edd6953a70c.pdf ER -