TY - JOUR ID - 66598 TI - In Vitro Effect of the Histone Deacetylase Inhibitor Valproic Acid on Viability and Apoptosis of the PLC/PRF5 Human Hepatocellular Carcinoma Cell Line JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Sanaei, Masumeh AU - Kavoosi, Fraidoon AU - Roustazadeh, Abazar AU - Shahsavani, Hossein AD - Research Center for Non-Communicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran. AD - Student of Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran. Y1 - 2018 PY - 2018 VL - 19 IS - 9 SP - 2507 EP - 2510 KW - Valproic Acid KW - Proliferation KW - Apoptosis KW - Hepatocellular carcinoma DO - 10.22034/APJCP.2018.19.9.2507 N2 - The nucleosome is the fundamental building block of eukaryotic chromatin formed by DNA and histone proteins.Chromatin modifications such as acetylation, methylation, and phosphorylation are necessary for protection,replication, and gene transcription. Histone deacetylases (HDACs) are a group of enzymes that remove acetyl groupsto re-establish positive charges on histones and aberrant deacetylation may lead to tumorigenesis in different tissues.Histone deacetylase inhibitors (HDACIs) are a class of chemotherapeutic agent that can reactivate gene expressionand inhibit the growth of tumor cells by histone deacetylase inhibition. HDACI valproic acid (VPA) has shown potentanticancer effects in vitro and in vivo. Previously, we reported that VAP can inhibit the growth and induce apoptosisof human colon carcinoma HT 29 and hepatocellular carcinoma HepG 2 cells. The aim of the present study was toaccess the effect of VPA on proliferation and apoptosis of the human hepatocellular carcinoma (HCC) PLC/PRF5 cellline. Materials and Methods: PLC/PRF5 cells were treated with VPA and then MTT and flow cytometry assays wereused to determine the effects on viability and apoptosis, respectively. Results: VPA inhibited cell growth and inducedapoptosis in PLC/PRF5 cells significantly. Discussion: Our results clearly demonstrated that VPA has inhibitory andapoptotic effects. Conclusion: VPA can significantly inhibit the growth of HCC cells and play a significant role inapoptosis induction. UR - https://journal.waocp.org/article_66598.html L1 - https://journal.waocp.org/article_66598_38fb138dba975ff8af558f7f2340c34d.pdf ER -