TY - JOUR ID - 67426 TI - Scrophularia Atropatana Extract Reverses TP53 Gene Promoter Hypermethylation and Decreases Survivin Antiapoptotic Gene Expression in Breast Cancer Cells JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Ghavifekr Fakhr, Mehrdad AU - Rezaie Kahkhaie, Kolsoum AU - Shanehbandi, Dariush AU - Farshdousti Hagh, Majid AU - Zarredar, Habib AU - Safarzadeh, Elham AU - Abdolrahimi Vind, Mina AU - Baradaran, Behzad AD - Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. AD - Department of Biochemistry, Faculty of Medical Sciences, Zabul University, Zabol, Iran. Y1 - 2018 PY - 2018 VL - 19 IS - 9 SP - 2599 EP - 2605 KW - BIRC5 KW - Epigenetics KW - p53 KW - Scrophularia atropatana KW - survivin   DO - 10.22034/APJCP.2018.19.9.2599 N2 - Background: In many cases of breast cancer, the aberrant methylation of TP53 gene leads to uncontrolled cellproliferation and apoptosis inhibition. Moreover, expression of oncogenes which are under the control of P53 proteincould be altered. Survivin as a conspicuous example of this category plays important roles in tumorigenesis, drugresistance and apoptosis inhibition. The present study was done to reveal the effects of Scrophularia atropatana extracton epigenetic situation of TP53 gene promoter and the expression levels of anti-apoptotic gene, survivin and its potentialfor production of cancer epi-drugs. Methods: Cytotoxic effect of dichloromethane extracts of Scrophularia plant onMCF-7 cell line was assessed in our previous study. Cell death ELISA (enzyme-linked immunosorbent assay) andTUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) tests were used to investigate the occurrence ofapoptosis in the treated cells. Methylation Specific PCR (MSP) was employed to assess the changes in methylationstatus of the TP53 gene promoter. Furthermore, quantitative real time PCR was utilized to evaluate the resulting changesin TP53 and survivin genes expression. Results: Cell death ELISA and TUNEL assays confirmed the occurrence ofapoptosis. MSP test revealed a significant change in the methylation status of TP53 promoter. QRT-PCR showedan increased TP53 gene expression in the treated cells while a significant decrease in survivin mRNA was evident.Conclusions: According to the outcomes, dichloromethane extract of S. atropatana returned the TP53 gene promoterhypermethylation to normal state. This plant could be a promising source for production of epi-drugs due to its apoptoticeffects and reversal of TP53 epigenetic alterations. UR - https://journal.waocp.org/article_67426.html L1 - https://journal.waocp.org/article_67426_c8703384d23471c5a79ec663eb656c75.pdf ER -