TY - JOUR ID - 69119 TI - Orthosiphon stamineus Extracts Inhibits Proliferation and Induces Apoptosis in Uterine Fibroid Cells JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Pauzi, Norzilawati AU - Mohd, Khamsah Suryati AU - Abdul Halim, Nor Hidayah AU - Ismail, Zhari AD - Faculty of Bioresources and Food Industry, University Sultan Zainal Abidin, Besut Campus, Besut, Terengganu, Malaysia. AD - School of Pharmaceutical Science, University Sains Malaysia, Penang, Malaysia. Y1 - 2018 PY - 2018 VL - 19 IS - 10 SP - 2737 EP - 2744 KW - Orthosiphon stamineus Benth KW - SK-UT-1 cells KW - Apoptosis KW - growth factor DO - 10.22034/APJCP.2018.19.10.2737 N2 - Objectives: The effects of water and 50% ethanolic-water extracts of Orthosiphon stamineus Benth (OS) on cellproliferation and apoptotic activity against uterine leiomyosarcoma (SK-UT-1) cells were investigated. Methods:Anti-proliferation effect was evaluated through cell cycle analysis whereas apoptotic activity was determined viascreening and quantifying using fluorescence microscopy and flow cytometric analysis, respectively. The effect ofextracts on molecular mechanism was studied using real-time reverse transcription polymerase chain reaction andWestern blotting. Results: Cell cycle flow cytometric analysis showed the induction of cell cycle arrests were behavesin a p53-independent manner. The examination using fluorescence microscopy and Annexin V flow cytometry revealedthe presence of morphological features of apoptotic bodies. Downregulation of anti-apoptotic gene (Bcl-2) supportsthe apoptotic activity of OS extracts although poorly induce PARP-1 cleavage in Western blot analysis. The extractsalso inhibit the SK-UT-1 growth by suppressing VEGF-A, TGF-β1 and PCNA genes, which involved in angiogenesisand cell proliferation. Conclusion: This study demonstrates that O. stamineus extracts are able to inhibit proliferationand induced apoptosis of uterine fibroid cells and is worth further investigation. UR - https://journal.waocp.org/article_69119.html L1 - https://journal.waocp.org/article_69119_a7e83405738ce760358f177bbbd50571.pdf ER -