TY - JOUR ID - 69703 TI - Gene Silencing of TGFβRII Can Inhibit Glioblastoma Cell Growth JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Ordoni aval, Farzane AU - Askarian Amiri, Shaghyegh AU - Azadmehr, Abbas AU - Oladnabi, Morteza AU - Saadat, Payam AU - Ebrahimi, Hadi AU - Baradaran, Behzad AU - Mansoori, Behzad AU - Pourabdolhossein, Fereshteh AU - Torabian, Pedram AU - Hajiahmadi, Mahmoud AD - Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. AD - Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran. AD - Mobility Impairment Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. AD - Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. AD - Department of Physiology, Babol University of Medical Sciences, Babol, Iran. AD - Department of Biostatistics and Epidemiology, Babol University of Medical Sciences, Babol, Iran. Y1 - 2018 PY - 2018 VL - 19 IS - 9 SP - 2681 EP - 2686 KW - TGF-β RII KW - siRNA KW - U-373 MG cell line KW - PDGF-BB KW - Glioblastoma DO - 10.22034/APJCP.2018.19.9.2681 N2 - Objective: Glioblastoma (GBM) is the most malignant and aggressive type of glioma, associated with a high rateof mortality. The transforming growth factor-β receptor II (TGFβ RII) is involved in glioma initiation and progression.On the other hand, TGFβ RII silencing is critical to the inhibition of GBM. Therefore, we aimed to determine theeffects of specific TGFβ RII siRNA on the survival of U-373MG cells. Methods: TGFβ RII siRNA was transfected,and qRT-PCR was performed to examine TGFβ RII mRNA expression. Cell survival was determined using colorimetricMTT assay, and platelet-derived growth factor-BB (PDGF-BB) level was measured in the culture supernatant usingELISA assay. Result: Our findings indicated that specific siRNAs could dose-dependently suppress TGFβ RII mRNAexpression after 48 hours. In addition, treatment with TGFβ RII siRNA significantly reduced tumor cell survival anddecreased the amount of PDGF-BB protein in the cell culture supernatant. Conclusion: Our results suggest that TGFβRII silencing can be a promising complementary treatment for glioma. UR - https://journal.waocp.org/article_69703.html L1 - https://journal.waocp.org/article_69703_26ccef11ecaea4d646f54cedd56f5433.pdf ER -