TY - JOUR ID - 69904 TI - Effect of a Copper (II) Complex on The Induction of Apoptosis in Human Hepatocellular Carcinoma Cells JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Rezaei, Azadeh AU - Khanamani Falahati-pour, Soudeh AU - Mohammadizadeh, Fatemeh AU - Hajizadeh, Mohammad Reza AU - Mirzaei, Mohammad Reza AU - Khoshdel, Alireza AU - Fahmidehkar, Mohammad Ali AU - Mahmoodi, Mehdi AD - Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. AD - Pistachio Safety Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. AD - Research Center of Advanced Technologies in Medicine, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran. AD - Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Y1 - 2018 PY - 2018 VL - 19 IS - 10 SP - 2877 EP - 2884 KW - Apoptosis KW - [Cu(L)(2imi)] complex KW - Cytotoxicity KW - Hepatocellular carcinoma KW - Mouse fibroblast L929 cells DO - 10.22034/APJCP.2018.19.10.2877 N2 - Objectives: In the present study, we aimed to identify the anti-proliferative potential of [Cu(L)(2imi)] complex[L = 2-(((5-chloro-2-oxyphenyl)imino)methyl)phenolato) and 2imi = 2-methyl imidazole] against HepG2 cells as anin vitro model of human hepatocellular carcinoma and normal mouse fibroblast L929 cells. Methods: The cytotoxicand apoptotic effects of [Cu(L)(2imi)] complex on HepG2 cells and normal fibroblasts (L929) were examined by MTTassay and flow cytometry, respectively. Results: Cytotoxicity induced by [Cu(L)(2imi)] complex was time dependent.Also, there was a positive correlation between cytotoxicity and an increase in Cu complex concentration. For HepG2cells, the cell viability percentage was 50% at 58 μg/mL after 24 h treatment, whereas in the same concentration andconditions, the viability percentage was surprisingly higher (about 100%) for L929 cells. Also, after 48 h treatment,the viability percentage of HepG2 cells at 55 μg/mL concentration was 50% in contrast with 89.3% for L929 cells inthe same conditions. Flow cytometry findings suggest that [Cu(L)(2imi)] complex is capable of decreasing cancer cellviability through apoptosis and did not efficiently activate the necrosis process. Conclusions: Finally, we found that[Cu(L)(2imi)] complex possess the potential for development as an anti-cancer drug for human hepatocellular carcinoma. UR - https://journal.waocp.org/article_69904.html L1 - https://journal.waocp.org/article_69904_531a2d8bddc07a4bf128db39d3160c10.pdf ER -