TY - JOUR ID - 77411 TI - Molecular Targeting of Notch Signaling Pathway by DAPT in Human Ovarian Cancer: Possible Anti Metastatic Effects JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Akbarzadeh, Maryam AU - Majidinia, Maryam AU - Fekri Aval, Sedigheh AU - Mahbub, Soltanali AU - Zarghami, Nosratollah AD - Department of Biochemistry, Higher Education Institute of Rab-Rashid, Tabriz, Iran. AD - Solid Tumor Research Center, Urmia University of Medical Science, Urmia, Iran. AD - Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Y1 - 2018 PY - 2018 VL - 19 IS - 12 SP - 3473 EP - 3477 KW - Notch signaling pathway KW - Metalloproteinases KW - Ovarian Cancer DO - 10.31557/APJCP.2018.19.12.3473 N2 - Background: Ovarian cancer is one of the most important gynecological malignancies, causing significant mortality.Recently, there has been extensive attention to the involvement of signaling cascades in its initiation/progression. In thisstudy, we focused on the possible role of Notch signal transduction in proliferation and metalloproteinase 2 and 9 functionin human ovarian cancer OVCAR-3 cells. Methods: MTT proliferation assays were used to evaluate effects of a DAPTinhibitor on cell proliferation. For measurement of Hes-1 mRNA levels, quantitative reverse transcription polymerasechain reaction (qRT-PCR) was applied following 48 h incubation with the inhibitor. In addition, metalloproteinase(MMPs) activity was assessed by zymography. Results: Inhibition of Notch signaling resulted in a significant reductionin OVCAR-3 cell proliferation. Additionally, DAPT treatment of cells significantly decreased Hes-1 mRNA levels(p < 0.05) as well as activity of MMP-2 and -9 (p < 0.05). Conclusion: Our results suggested that suppression of Notchsignaling by a specific inhibitor can effectively decrease proliferation and the potential for metastasis of OVCAR-3 cellsvia a reduction in the activity of metalloproteinases 2 and 9. Thus, pharmacological targeting of the Notch signalingpathway could be a promising future treatment for ovarian cancer. UR - https://journal.waocp.org/article_77411.html L1 - https://journal.waocp.org/article_77411_084e1b8c027dd7fa5f7a3ed82a6ace46.pdf ER -