TY - JOUR ID - 81620 TI - Evaluation of Cisplatin Efficacy on HepG2 and E. coli Cells under Acidic Conditions JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Babaei, Faezeh AU - Ebrahimi Shahmabadi, Hasan AU - Rajabi, Mohammad Reza AU - Haddad Kashani, Hamed AU - Izadpanah, Fatemeh AD - Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran. AD - Department of Microbiology, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. AD - Faculty of Medicine, Shahed University of Medical Sciences, Tehran, Iran. AD - Food and Drug Laboratory Research Center and Food and Drug Reference Control Laboratories Center, Food and Drug Administration of Iran, MOH and ME, Tehran, Iran. Y1 - 2019 PY - 2019 VL - 20 IS - 3 SP - 723 EP - 726 KW - Acidic cisplatin KW - HepG2 KW - MIC assay KW - MTT assay KW - Standard cisplatin DO - 10.31557/APJCP.2019.20.3.723 N2 - Background: Cisplatin (Cispt) is a common anticancer drug for the treatment of several malignancies, includinghepatocarcinoma. However, this drug suffers from instability in aqueous solutions. The study aimed to evaluate cisplatinefficacy on HepG2 and E. coli cells under an acidic condition. Methods: Acidic Cispt was prepared via incubation inacidic condition (pH=2) for a month duration. The chemical structure of the acidic Cispt was evaluated by using FourierTransform Infrared Spectroscopy (FTIR) method. The cytotoxicity of the standard and acidic Cispt were then determinedby 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and minimum inhibitory concentration (MIC)assays on HepG2 and E. coli cells, respectively. Results: After preparing of acidic Cispt, its chemical structure wasdetermined by FTIR method. In addition, cytotoxicity effects of Cispt in the standard and acidic forms were calculated58 ± 2.9 and 65 ± 3.25 μM, respectively. MIC results also confirmed the results of MTT assay. MIC results for thestandard and acidic Cispt were estimated 9.5 ± 0.47 and 9.8 ± 0.49 μM, respectively. Conclusion: Preparing Cispt inacidic condition not only did not degrade the drug, but also kept the potency of the drug approximately equal to parentdrug. Regarding the instability issues of Cispt, keeping Cispt in acidic condition could be a promising approach topreserve its efficacy. UR - https://journal.waocp.org/article_81620.html L1 - https://journal.waocp.org/article_81620_e525d17e475a1f8d0a0a9686e674cc4f.pdf ER -