TY - JOUR ID - 82234 TI - Anti-Proliferative Activity of Triterpenes Isolated from Clinicanthus nutans on Hep-G2 Liver Cancer Cells JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Zakaria, Khairun Najwa AU - Amid, Azura AU - Zakaria, Zubaidah AU - Jamal, Parveen AU - Ismail, Azli AD - Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Kuala Lumpur, Malaysia. AD - International Institute for Halal Research and Training, Level 3, KICT Building, International Islamic University Malaysia, Kuala Lumpur, Malaysia. AD - Haematology Unit, Cancer Research Centre, Institute for Medical Research, Kuala Lumpur, Malaysia. Y1 - 2019 PY - 2019 VL - 20 IS - 2 SP - 563 EP - 567 KW - Clinicanthus nutans KW - anti-proliferative KW - triterpenes KW - Liver cancer DO - 10.31557/APJCP.2019.20.2.563 N2 - Problem statement: Clinicanthus nutans has been used by Malaysian since long time ago. It is used to treat manydiseases including cancer. Many studies carried out on its crude extract but no clear report on the specific secondarymetabolites responsible for its nature in treating selected diseases. Objective: This study aims to confirm the practicecarried out by many people on the usage of Clinicanthus nutans in treating cancer. Methods: C. nutans leaves wereextracted by methanol. Thin layer chromatography was used to identify the suitable solvent for fractions separation.The fractions were then separated at larger volume using gravity column chromatography. Each fraction was tested onits anti-proliferative activity on Hep-G2 liver cancer cells by MTT assay. The phytochemical screening was carried outto identify the bioactive compound based on qualitative analysis. Results: The fraction 2 (F2) of C. nutans showed thelowest IC50 value of 1.73 μg/ml against Hep-G2 cancer cells, and it is identified as triterpenes. Conclusion: The fractionF2 identified as triterpenes isolated from C. nutans has potential as an anti-proliferative agent against liver cancer. UR - https://journal.waocp.org/article_82234.html L1 - https://journal.waocp.org/article_82234_4aeb6476e19fdef38c4f4f32642152be.pdf ER -