TY - JOUR ID - 82235 TI - Prevalence of Intestinal Parasitic Infection in Cancer, Organ Transplant and Primary Immunodeficiency Patients in Tehran, Iran JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Esteghamati, Abdoulreza AU - Khanaliha, Khadijeh AU - Bokharaei-Salim, Farah AU - Sayyahfar, Shirin AU - Ghaderipour, Masoomeh AD - Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran. AD - Departments of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Y1 - 2019 PY - 2019 VL - 20 IS - 2 SP - 495 EP - 501 KW - Immunodeficiency KW - Transplant KW - cancer KW - opportunistic KW - parasite infection DO - 10.31557/APJCP.2019.20.2.495 N2 - Background: Intestinal parasitic infection in immunodeficient patients especially those with impaired cellularimmunity, like neoplasia, renal or heart transplant needs careful consideration. The objective of this study is to evaluatethe prevalence of intestinal parasites in different group of patients including cancer patients; organ transplants recipients,and primary immunodeficiency patients. Methods: Stool samples from 190 patients including 80 patients with PrimaryImmunodeficiency, 85 cancer patients and 25 organ transplant recipients were collected; a direct examination withPhosphate buffered saline (PBS) and formalin ether concentration was performed. The DNA was extracted fromparasitologically confirmed patients and nested PCR and sequencing was performed and new obtained sequences ofCryptosporidium parvum and Enterocytozoon bieneusi were compared with deposited ones. Results: In general, theprevalence of parasites was 26/80 (32.5%) in primary immunodeficiency, 22/85(25.9%) in cancer group, and 7/25(28%) in organ transplant. The prevalence of intestinal parasitic infections in primary immunodeficiency patientswere Blastocystis hominis 13 (16.2%), Giardia lamblia 10 (12.5%), Cryptosporidium 1(1.2%), Chilomastix mesnilii 1(1.2%), Dientamoeba fragilis 1(1.2%). Of 25 organ transplants, 6 (24%) Cryptosporidium sp were found, all of whichwere confirmed as Cryptosporidium parvum and one case of Microspora in a heart transplant recipient was confirmedas Enterocytozoon bieneusi by PCR sequencing. The predominant intestinal parasitic infection in cancer patients was19 (22.3%) Blastocystis hominis followed by two (2.3%) Giardia lamblia and one Dientamoeba fragilis 1 (1.1%).Conclusion: The high rate of infection with Blastocystis hominis was found in cancer patients especially colorectalcancer patients, so careful consideration should be given by physicians. Cryptosporidium sp was found to be the majorcause of parasitic intestinal infection in patients with organ transplant compared to primary immunodeficiency patients;so transplant recipients undergoing immunosuppressive therapy should be considered as a risk group for acquiringmicrosporidiosis and Cryptosporidium infection. UR - https://journal.waocp.org/article_82235.html L1 - https://journal.waocp.org/article_82235_2e78ab85e3af55dafe92c00799f51a99.pdf ER -