TY - JOUR ID - 82579 TI - Study on JV Virus in Patients with Colon Cancer Type Adenocarcinoma JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Haghi Navand, Azadeh AU - Teimoori, Ali AU - Makvandi, Manoochehr AU - Nisi, Nilofar AU - Seyedian, Seyed Saeid AU - Ranjbari, Nastaran AU - Ahmadi Angali, Kambiz AU - Keyani, Hadis AU - Tabasi, Maryam AU - Pourjabari, keyvan AD - Infectious and Tropical Diseases Research Center Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. AD - Virology Department, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. AD - Alimentary Tract Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. AD - Department of Pathology, Imam Khomeini Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. AD - Department of Biostatistic, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Y1 - 2019 PY - 2019 VL - 20 IS - 4 SP - 1147 EP - 1151 KW - JC Virus KW - colorectal adenocarcinoma KW - Polymerase Chine Reaction DO - 10.31557/APJCP.2019.20.4.1147 N2 - Colorectal cancer is the most repetitious malignancies with high mortality worldwide. JC virus (JCV) is ubiquitousPolyomavirus, with seroprevalence rates ranging from 70% to 90% in adult population. Recently the role of JCV havebeen reported in many malignant tumors worldwide. The association of JCV was reported in patients with colon andrectum cancers. Thus this study was conducted to evaluate the association of JCV DNA in patients with colon cancertype Adenocarcinoma. Material and Methods: A total of 120 formalin-fixed paraffin-embedded tissue blocks sampleswere collected including 20/40(50%) males, 20/40(50%) females patients with Colorectal Cancer(CRC), and 80 (50%males, 50% females) patients with benign tumor as a control. DNA was extracted for all the samples. Nested PCR wascarried out for detection of Vp1/T-Ag junction genome in JCV genome by Nested-PCR assay. Randomly, PCR productsof 6 samples were sequenced to analysis the partial JCV DNA. The phylogeny tree was constructed to determinehomology identity with other JCV. Results: 4/40(10%) samples of test group and 10/80 (12.5%) of control sampleswere positive for JCV DNA (P= 0.69). Out of 4 samples positive for JC DNA, 3(7.5%) were males and 1(2.4%) female(P=0.29). The frequency of JCV DNA in age group> 50 years was 4/32(10%), while in age group (0%) (p= 0.29). Conclusion: prevalence of JCV DNA was among 10% patients with CRC and 12.5% benign tumors(p=0.69). The distribution of JCV DNA was among 7.5% male and 2.5% female (p= 0.29). The frequency of JCVDNA was among 10% cases of age group >50 years and 0% of age group protein expression might explain the increased risk of colorectal cancer and requires further investigation. UR - https://journal.waocp.org/article_82579.html L1 - https://journal.waocp.org/article_82579_7cc3daa760515bee500a2ca0a5efd705.pdf ER -