TY - JOUR ID - 86807 TI - Bioactive Compounds in the Ethanol Extract of Marine Sponge Stylissa carteri Demonstrates Potential Anti-Cancer Activity in Breast Cancer Cells JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Bashari, Muhammad Hasan AU - Huda, Fathul AU - Tartila, Tamia S AU - Shabrina, Sarah AU - Putri, Tenny AU - Qomarilla, Nurul AU - Atmaja, Harold AU - Subhan, Beginer AU - Sudji, Ikhwan Resmala AU - Meiyanto, Edy AD - Department of Biomedical Sciences, Division of Pharmacology and Therapy, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia. AD - Oncology and Stem Cell Working Group, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia. AD - Undergraduate Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia. AD - Laboratory of Advanced Biomedicine, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia. AD - Department of Marine Science and Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor, Indonesia. AD - Laboratory of Biomedicine, Faculty of Medicine, Universitas Andalas, Padang, Indonesia. AD - Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Y1 - 2019 PY - 2019 VL - 20 IS - 4 SP - 1199 EP - 1206 KW - anti-cancer activity KW - breast cancer KW - marine sponge KW - Stylissa carteri DO - 10.31557/APJCP.2019.20.4.1199 N2 - Objective: Despite advanced treatment options available, drug resistance develops in breast cancer (BC) patientsrequiring novel effective drugs. Stylissa carteri, a marine sponge predominantly living in Indonesia territories, hasnot been extensively studied as anti-cancer. Therefore, this study targeted to assess the anti-tumor activity of theethanol extract of S. carteri in BC cells. Methods: S. carteri was collected from Pramuka Island, at Kepulauan SeribuNational Park, Jakarta, Indonesia and extracted using ethanol. Different BC cells including MDA MB 231, MDAMB 468, SKBR3, HCC-1954 and MCF-7 cells were treated with this extract for cytotoxic analysis using MTT assay.Spheroid growth assay and apoptosis assay were conducted in HCC-1954 cells. In addition, cell migration analysis andsynergistic activity with doxorubicin or paclitaxel were conducted in MDA MB 231 cells. This extract was subjectedalso for GC-MS analysis. Results: The results show that ethanol extract of S. carteri demonstrated a cytotoxic activityin BC cells. The IC50 of this extract was lower 15 μg/ml in MDA MB 231, MDA MB 468, SKBR3, and HCC-1954cells. Moreover, this extract inhibited spheroids growth and induced apoptosis in HCC-1954 cells. It inhibited cellmigration and demonstrated a synergistic activity with doxorubicin or paclitaxel on triggering cell death in MDA MB231 cells. Furthermore, GC-MS analysis indicated that this extract contained 1,2-Benzenediol, Dibutyl phthalate and9,12-Octadecadienoic acid, ethyl ester. Conclusion: Our preliminary data indicate a potential anti-tumor activity ofethanol extract of S. carteri in breast cancer cells. UR - https://journal.waocp.org/article_86807.html L1 - https://journal.waocp.org/article_86807_cd42585884b6645b3c32e401648270d7.pdf ER -