TY - JOUR ID - 87870 TI - C-MYC and BCL2: Correlation between Protein Over-Expression and Gene Translocation and Impact on Outcome in Diffuse Large B Cell Lymphoma JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Mohammed, Amrallah A AU - Rashed, Hayam E AU - Abdelrahman, Aziza E AU - Obaya, Ahmed A AU - Toam, Mostafa AU - Abdel Nour, Hanim M AU - Abdelhamid, Mohamed I AU - Elsayed, Fifi Mostafa AD - Department of Medical Oncology, Faculty of Medicine, Zagazig University, Egypt. AD - Department of Pathology, Faculty of Medicine, Zagazig University, Egypt. AD - Department of Clinical Oncology, Faculty of Medicine, Zagazig University, Egypt. AD - Department of Biochemistry, Faculty of Medicine, Zagazig University, Egypt. AD - Department of General Surgery, Faculty of Medicine, Zagazig University, Egypt. AD - Department of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Suez Canal University, Egypt. Y1 - 2019 PY - 2019 VL - 20 IS - 5 SP - 1463 EP - 1470 KW - Keyword: Double expressor lymphoma KW - Double hit lymphoma KW - immunohistochemistry KW - Myc KW - BCL2 DO - 10.31557/APJCP.2019.20.5.1463 N2 - Background: Due to lack of availability of gene expression profiling (GEP) for most developing countries andclinicians; the immunohistochemistry (IHC) is mostly used in the clinical application. The aim of our study is to checkthe possibility of using IHC to detect MYC and BCL2 in our patients with diffuse large B-cell lymphoma (DLBCL)instead of GEP to stratify them into high and low-risk groups. This will help in a proper treatment choice of subsequentimprovement in the survival outcome. Method: During the study period, 90 DLBCL patients were eligible. MYC andBCL2 evaluated by IHC and gene rearrangement by real-time PCR (RT-PCR) and correlated with clinical-pathologicalfeatures and survival. Results: Through IHC, the expression of MYC, BCL2, and double expression was detectedin 35.6%, 46.7% and 30% of patients, respectively. While by RT-PCR, it was 4.53±0.74 for MYC compared with2.18±0.78 for BCL-2. Most patients with BCL2+/MYC+; double-expressor and double-hit lymphomas (DEL andDHL) had high stage (III, IV), more extra-nodal involvement, (P value <0.001) and intermediate to high InternationalPrognostic Index (IPI) risk profile (P-value <0.001). The median overall survival was 14 months and 6 months for DELand DHL, respectively. While all patients with DHL died during the follow-up period, the median PFS were only 2months for DEL. There was a statistically significant correlation between mRNA of MYC and BCL2 with their proteinexpression (p<0.001). Conclusion: Our results confirmed the unique characters and poor outcome associated withDEL and DHL mandated the need for more intense therapy and not the standard protocol. Moreover, the significantcorrelation between protein overexpression and gene rearrangement may open the door for the possibility to use IHCinstead of RT-PCR in developing countries. UR - https://journal.waocp.org/article_87870.html L1 - https://journal.waocp.org/article_87870_09b8cf89526f0321d498a48002600378.pdf ER -