TY - JOUR ID - 88605 TI - Establishment and Characterization of a New Triple Negative Breast Cancer Cell Line from an Iranian Breast Cancer Tissue JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Ghaderi, Farzaneh AU - Mehdipour, Fereshteh AU - Hosseini, Ahmad AU - Talei, Abdolrasoul AU - Ghaderi, Abbas AD - Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. AD - Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. AD - Department of Surgery, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Y1 - 2019 PY - 2019 VL - 20 IS - 6 SP - 1683 EP - 1689 KW - breast cancer KW - Cell line establishment KW - Triple-negative DO - 10.31557/APJCP.2019.20.6.1683 N2 - Breast cancer is the most common malignancy and the leading cause of cancer-related death among womenworldwide. The underlying mechanisms for breast cancer development, especially in young women, are not completelyunderstood. Although there are several experimental models to understand the biology of breast cancer such asimmortalized cell lines, many of these cell lines have been in culture for decades and most of them have been derivedfrom Caucasians or African-Americans. So, it is required to establish a new cell line derived from primary tumors andAsian women. In this study Pari-Institute for Cancer Research (Pari-ICR) was derived from the primary breast tumorof a 36-years old patient with invasive ductal carcinoma. We characterized the cell line by examining morphology,expression of different markers, and functional profile. Immunocytochemistry showed that this cell line does not expressestrogen and progesterone receptors as well as human epidermal growth factor receptor 2 (HER2). Pari-ICR cell lineexpresses high levels of Vimentin, Ezrin, and S100 but does not express EpCAM, Cytokeratin19, Pan-cytokeratin,Nestin, and Desmin. Its doubling time of Pari-ICR was about 22h and was able to grow as colonies in soft agar. Itdisplayed a higher ability of migration and invasion in comparison with MCF-7 cell line. This breast cancer cell linecan serve as a model for understanding the molecular mechanisms of breast carcinogenesis. Moreover, it can be usedas an appropriate resource to find novel biomarkers or assess new drugs. UR - https://journal.waocp.org/article_88605.html L1 - https://journal.waocp.org/article_88605_409efb77569a52246255986bba038925.pdf ER -