TY - JOUR ID - 88668 TI - Bevacizumab and CCR2 Inhibitor Nanoparticles Induce Cytotoxicity-Mediated Apoptosis in Doxorubicin-Treated Hepatic and Non-Small Lung Cancer Cells JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Abd-Rabou, Ahmed A AU - Ahmed, Hanaa H AD - Hormones Department, Medical Research Division, National Research Centre, Giza, Egypt. Y1 - 2019 PY - 2019 VL - 20 IS - 7 SP - 2225 EP - 2238 KW - Bevacizumab (avastin) KW - CCR2 antagonist KW - non-small cell lung cancer KW - Hepatocellular carcinoma KW - Cytotoxicity DO - 10.31557/APJCP.2019.20.7.2225 N2 - Non-small cell lung cancer (NSCLC) and hepatocellular carcinoma (HCC) are very common in certain populationaround the world. Despite the recent advances in their diagnosis and therapy, their prognosis remains poor due to thedevelopment resistance to drug. Although doxorubicin (DOX) is considered to be one of the most anti-solid tumordrugs, developed resistance is contributing to unsuccessful outcome. The rationale of the current study is to explorethe sensitizing capability of the DOX-treated cancer cells using the anticancer agents; bevacizumab (avastin; AV) andCCR2 inhibitor (CR) in their free- and nano-formulations. Here, the average size, polydispersity index (PDI), zetapotential, and entrpment effeciency (EE%) of the synthesized nanoparticles were measured. We investigated the effectof these platforms on the proliferation, apoptosis, necrosis, nitric oxide (NO), malondialdehyde (MDA), and zinc levelsof human HCC (HepG2 and Huh-7) and NSCLC (A549) cancer cell lines. Glucose consumption rates using Huh-7and A549 cancer cells were tested upon treatments. We demonstrated that AV and CR nano-treatments significantlysuppressed A549 cell viability and activated apoptosis by NO level elevation. We concluded that AVCR NP plusDOX significantly induces A549 cytotoxicity-mediated apoptosis more than Huh-7 and HepG2 cells. This drug-drugnano-combination induced Huh-7 cytotoxicity-mediated apoptosis more than HepG2 cells. In conclusion, AVCR NPsensitized DOX-treated A549 and Huh-7 cells through reactive oxygen species (ROS)-stimulated apoptosis. Takentogether, our data suggested that the CR plus AV nano-platforms would be a potential personalized medicine-basedstrategy for treating CCR2-positive NSCLC and HCC patients in the near future. UR - https://journal.waocp.org/article_88668.html L1 - https://journal.waocp.org/article_88668_4d1966c1d8f30a9abdc826e8274d41e3.pdf ER -