TY - JOUR ID - 88694 TI - Combined Hyperthermia and Radiation Therapy for Treatment of Hepatocellular Carcinoma JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Talaat, Roba M AU - Abo-Zeid, Tamer M AU - Abo-Elfadl, Mahmoud T AU - El-Maadawy, Eman A AU - Hassanin, Mona M AD - Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt. AD - Cancer Biology and Genetics Laboratory, Center of Excellence for Advanced Sciences, National Research Center, Egypt. AD - Egyptian Atomic Energy Authority, Department of Radioisotope, Cairo, Egypt. Y1 - 2019 PY - 2019 VL - 20 IS - 8 SP - 2303 EP - 2310 KW - Hyperthermia KW - radiotherapy KW - Cytotoxicity KW - Angiogenesis KW - Apoptosis DO - 10.31557/APJCP.2019.20.8.2303 N2 - Background: There is no doubt that hyperthermia is one of the powerful radiosensitizers. Finding a proper mechanismworking in hyperthermia/radiation combination is still pronounced challenge. Objectives: This study is focusing onthe anti-cancer activities (anti-proliferative, anti-angiogenic and antiapoptotic) of thermoradiotherapy. Materials andMethods: Liver cancer cell line (HepG2) was treated by 37oC, 40oC and 43oC hyperthermia degrees combined withthree radiation doses (2 Gy, 4 Gy and 8 Gy) for 24, 48 and 72 hrs. Cell viability, apoptotic/necrotic cell screening,apoptotic (BAX and FasL) and antiapoptotic (BCL-2 and GRP78) genes, and pro-angiogenic mediators [vascularendothelial- (VEGF) and Platelet derived-growth factors (PDGF) ware investigated. Results: Our data showed that 40oCtemperature combined with 4 Gy radiation gives a significant decrease (p<0.05) in cell viability. Maximum cytotoxicitywas reported 48 hr post-treatment followed by slight restoration of cell viability after 72 hr. Compared with untreatedcells, only 5% of viable cells with a high percentage of apoptotic (31%) and necrotic (63%) cells were demonstratedin 40oC/4 Gy/48 hr group. Expression of pro-apoptotic genes (BAX and FasL) were increased after hyperthermia withapparent elevation in 40oC/4 Gy/48 hr group coincides with moderate expression of antiapoptotic BCL-2 and GRP78genes. A significant reduction (p<0.001; p<0.05) in VEGF and PDGF levels; respectively was shown at 40oC/4 Gy/48hr group. Conclusions: This pilot study proposed 40oC mild temperature hyperthermia as a favorable hyperthermalcondition with 4 Gy radiotherapy in HCC treatment. A further research has to be performed considering an applicationof more than one session of radiothermal therapy at 40oC/4 Gy for total abrogation of cancer cells. UR - https://journal.waocp.org/article_88694.html L1 - https://journal.waocp.org/article_88694_ebdbab067bf732dcbe383ef0824efc45.pdf ER -