TY - JOUR ID - 88717 TI - Effect of Pentoxifylline on Staurosporine-Induced Neurite Elongation in PC12 Cells JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Diojan, Loghman AU - Zhaleh, Hossein AU - Azadbakht, Mehri AU - Bidmeshkipour, Ali AU - Khodamoradi, Ehsan AD - Department of Radiology and Nuclear Medicine, School of Allied Medical Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran. AD - Substance Abuse Prevention Research Center, Institute of Health, Kermanshah University of Medical Sciences, Kermanshah, Iran. AD - Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran. Y1 - 2019 PY - 2019 VL - 20 IS - 9 SP - 2633 EP - 2638 KW - pentoxifylline KW - cell death KW - Neurite KW - staurosporine DO - 10.31557/APJCP.2019.20.9.2633 N2 - Objective: Pentoxifylline enhances neurite elongation in PC12 cells. This study investigated the effects of pentoxifylline on staurosporine-induced neurite elongation in PC12 cells. Materials and Methods: There were five treatment groups, including treatment group I (1 nM), treatment group II (10 nM), treatment group III (100 nM), treatment group IV (1uM), and treatment group V (10 mM of pentoxifylline), together with 214 nM staurosporine for a range of time (6, 12 and 24 hours). Cells only treated with staurosporine at a concentration of 214 nM were used as the control group. Cell proliferation, cell death, immunocytochemistry assay, and Total Neurite Length were assessed. Results: The results showed that pentoxifylline increased cell viability (p<0.05) in a dose- and time-dependent manner, and cell death assay showed that cell death decreased in a dose- and time-dependent manner (p<0.05). TNL increased significantly compared with control cells (p<0.05). Immunocytochemistry assay showed that pentoxifylline at low and high concentrations enhanced β-tubulin III and GFAP protein expression compared with control cells. Conclusion: It can be concluded that pentoxifylline has positive effects on the staurosporine-induced neurite outgrowth process in PC12 cells. UR - https://journal.waocp.org/article_88717.html L1 - https://journal.waocp.org/article_88717_a7c6332218503da89e1a00c1c2765411.pdf ER -