TY - JOUR ID - 88790 TI - Comparative Study of Blood, Tissue and Serum Levels of Carcinoembryonic Antigen (CEA) Detection in Breast Cancer JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Moazzezy, Neda AU - Bouzari, Saeid AU - Oloomi, Mana AD - Molecular Biology Unit, Pasteur Institute of Iran, Tehran, Iran. Y1 - 2019 PY - 2019 VL - 20 IS - 10 SP - 2979 EP - 2985 KW - CEA KW - RT-PCR KW - breast cancer KW - Prognosis DO - 10.31557/APJCP.2019.20.10.2979 N2 - Background: Carcinoembryonic antigen (CEA) detection was evaluated in breast cancer (BC). The statistical correlation between the CEA mRNA and clinico-pathological features in the peripheral blood (PB) and tissue samples of BC was assessed. Materials and Methods: RT-PCR (Reverse transcription-polymerase chain reaction) analysis was applied to study the expression of CEA in PB of 30 healthy females and 30 patients with operable BC before receiving any therapy, as well as in the tissue of 30 BC patients. Results: CEA was observed in a number of normal subjects, but there was a significant difference between the patients and controls. The detected CEA mRNA from tissue samples were the same as PB of patients and a correlation was observed between the CEA mRNA in PB and tissue samples (Pearson chi-square = 8.62, P=0.003). In the PB, CEA mRNA was significantly different in HER-2 (-)/HR (+) compare with HER-2(+)/HR (-) tumor group (p=0.026). Finally, CEA in serum was also significantly different in HER-2(-)/HR (+) compared with HER-2(+)/HR (+) and HER-2(+)/HR (-) subtypes (p=0.008 and p=0.043, respectively). Conclusion: CEA mRNA evaluation is diagnostically valuable as a breast cancer marker. Additionally, CEA can significantly improve the sensitivity of diagnosis. UR - https://journal.waocp.org/article_88790.html L1 - https://journal.waocp.org/article_88790_a8d1b1172d2f2a5d0c0d0281b38db1a4.pdf ER -