TY - JOUR ID - 88815 TI - The Role of the LY294002 - A Non-Selective Inhibitor of Phosphatidylinositol 3-Kinase (PI3K) Pathway- in Cell Survival and Proliferation in Cell Line SCC-25 JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Duarte, Andressa AU - Silveira, Giórgia Gobbi AU - Soave, Danilo Figueiredo AU - Costa, João Paulo Oliveira AU - Silva, Alfredo Ribeiro AD - Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School, University of São Paulo, Brazil. Y1 - 2019 PY - 2019 VL - 20 IS - 11 SP - 3377 EP - 3383 KW - PI3K KW - Akt KW - Cox KW - GLUT1 KW - Bcl-2 DO - 10.31557/APJCP.2019.20.11.3377 N2 - The activation of PI3K further activates subsequent regulatory pathways, which are activated via AKT phosphorylation. AKT is closely related to the Bcl-2 family, a protein known to be involved in cell survival. AKT also has a relationship with inflammatory and glycolytic mediators. The present work aimed to evaluate the relationship between the PI3K/AKT pathway, cell survival/proliferation, inflammatory mediators and the glycolytic pathway in oral squamous cell carcinoma. All experiments were performed in the SCC25 oral squamous cell carcinoma cell line. In the presence or absence of PI3K pathway inhibitors, we analyzed the protein expression of pAKT and AKT; X-linked inhibitor of apoptosis protein; Bcl-2-associated death promoter; Bcl-2-like protein two inhibitor; cyclooxygenase 1; cyclooxygenase-2; and glycoprotein-associated glucose transporter 1. For the functional characterization of treated or untreated cells, we also performed matrix invasion assays, cell migration assays, and cell proliferation assays. Our results demonstrated that activation of the PI3K/AKT pathway is directly related to members of the Bcl-2 family and GLUT1, but not the inflammatory mediators COX1 and COX2. Our data suggest that the PI3K/AKT pathway is related to cell survival and proliferation in oral squamous cell carcinoma through its interaction with Bcl-2 family members.   UR - https://journal.waocp.org/article_88815.html L1 - https://journal.waocp.org/article_88815_c3ebab02a5cf89d2c488de876b6f2e22.pdf ER -