TY - JOUR ID - 88930 TI - Inhibitory Effect of Polyclonal Antibodies Against HER3 Extracellular Subdomains on Breast Cancer Cell Lines JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Mansouri-Fard, Samaneh AU - Ghaedi, Mojgan AU - Shokri, Mohammad-Reza AU - Bahadori, Tannaz AU - Khoshnoodi, Jalal AU - Golsaz-Shirazi, Forough AU - Jeddi-Tehrani, Mahmood AU - Amiri, Mohammad Mehdi AU - Shokri, Fazel AD - Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. AD - Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. AD - Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. Y1 - 2020 PY - 2020 VL - 21 IS - 2 SP - 439 EP - 447 KW - breast cancer KW - Fusion protein KW - HER3 KW - Polyclonal antibody DO - 10.31557/APJCP.2020.21.2.439 N2 - Objective: Human epidermal growth factor receptor 3 (HER3) is a unique member of the tyrosine kinase receptors with an inactive kinase domain and is the preferable dimerization partner for HER2 which lead to potent tumorigenic signaling. Methods: In this study, the expression plasmids coding for the human HER3 subdomains were transfected into CHO-K1 cells. Produced proteins were characterized by ELISA and SDS-PAGE. Rabbits were immunized and produced polyclonal antibodies (pAbs) that were characterized by ELISA, Immunoblotting and flowcytometry and their inhibitory effects were assessed by XTT on BT-474 and JIMT-1 breast cancer cell lines. Result: The recombinant subdomains were highly immunogenic in rabbits. The pAbs reacted with the recombinant subdomains as well as commercial HER3 and the native receptor on tumor cell membranes and could significantly inhibit growth of Trastuzumab sensitive (BT-474) and resistant (JIMT-1) breast cancer cell lines in vitro. Conclusion: It seems that HER3 extra cellular domains (ECD) induce a strong anti-tumor antibody response and may prove to be potentially useful for immunotherapeutic applications.   UR - https://journal.waocp.org/article_88930.html L1 - https://journal.waocp.org/article_88930_1c08f40a1bc59e9b3cd2126aa53ba476.pdf ER -