TY - JOUR ID - 89058 TI - Promoter Hypermethylation of LATS2 Gene in Oral Squamous Cell Carcinoma (OSCC) Among North Indian Population. JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Goel, Harsh AU - Singhal, Saloni AU - Mathur, Runjhun AU - Syeda, Saima AU - Gupta, Rishi Kumar AU - Kumar, Anshuman AU - Shrivastava, Anju AU - Jha, Abhimanyu Kumar AD - Department of Biotechnology, Institute of Applied Medicines and Research Ghaziabad, Uttar Pradesh, India. AD - Dr. A.P.J. Abdul Kalam Technical University, Lucknow, Uttar Pradesh, India. AD - Department of Zoology, Delhi University, India. AD - Sh. Jagannath Charitable Cancer Hospital, Ghaziabad, Uttar Pradesh, India. AD - Dharamshila Cancer Hospital and Research Centre, New Delhi, India. Y1 - 2020 PY - 2020 VL - 21 IS - 5 SP - 1283 EP - 1287 KW - Keywords: Oral squamous cell carcinoma (OSCC) KW - Epigenetic changes KW - Promoter hypermethylation KW - Methylation-Specific PCR (MSP) KW - Biomarker DO - 10.31557/APJCP.2020.21.5.1283 N2 - ABSTRACTLarge Tumor Suppressor (LATS2) gene are Tumor Suppressor gene, linked with epigenetic modifications. LATS2 promoter hypermethylation is an important epigenetic silencing mechanism leading to cancer. Cancer is the most common, vicious and dangerously increasing diseases of the world today, associated with high morbidity and mortality. Oral cancers (OC) are the blazing universal dilemma and is the sixth most frequent cancer observed in Indian population. Tobacco consumption is the main cause of the increase in OSCC. The association between LATS2 in the pathogenesis of cancers propose that their combination might be studied as a possible molecular marker for particular subgroups of patients. Therefore, the present study tried to investigate whether LATS2 promoter methylation was associated with oral squamous cell carcinoma (OSCC) in North Indian subjects. DNA methylation quantitative studies of LATS2 Tumor Suppressor genes were performed by methylation-specific polymerase chain reaction (MSP). 38 out of 70 patients (55 %) were found to be methylated for LATS2 gene, a statistically significant result was obtained (p-value < 0.005) for LATS2 genes. The results suggest that epigenetic changes may be related to the down-regulation of LATS2 expression. It can be concluded that LATS2 gene plays a significant role in the diagnosis of cancer and provide a better alternative as a diagnostic biomarker. Our data infer that a low LATS2 expression due to methylation may contribute to the cancer progression and could be useful for the diagnosis of OSCC. Therefore, investigation of promoter methylation in such genes may provide a biomarker which may prove to be useful in early detection of Oral Cancer.Keywords: Oral squamous cell carcinoma (OSCC), Epigenetic changes, LATS2 gene, Promoter Hypermethylation, Methylation-Specific PCR (MSP), BiomarkersAbbreviations: OSCC- Oral Squamous Cell Carcinoma; DNA- Deoxyribonucleic acid; LATS-Large Tumor Suppressor (gene); MSP-Methylation-Specific Polymerase Chain Reaction UR - https://journal.waocp.org/article_89058.html L1 - https://journal.waocp.org/article_89058_f30fc799f59fa1e35d49d2a7f1538af5.pdf ER -