TY - JOUR ID - 89172 TI - Anti-Migration and Invasion Effects of Astaxanthin against A172 Human Glioblastoma Cell Line JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Siangcham, Tanapan AU - Vivithanaporn, Pornpun AU - Sangpairoj, Kant AD - Faculty of Allied Science, Burapha University, Chonburi, Thailand. AD - Chakri Naruebodindra Medical Institute, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Samut Prakan, Thailand. AD - Division of Anatomy, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani, Thailand. Y1 - 2020 PY - 2020 VL - 21 IS - 7 SP - 2029 EP - 2033 KW - astaxanthin KW - Glioblastoma KW - migration KW - Invasion KW - matrix metalloproteinase DO - 10.31557/APJCP.2020.21.7.2029 N2 - Objectives: The study was to investigate anti-migration and invasion effects of astaxanthin (ATX), a natural carotenoid derivative distributed in marine environments, against A172 human glioblastoma cells. Materials and Methods: Cell viability after ATX treatment was measured by MTT assays. Tumor cell migration and invasion were observed by scratch and Boyden chamber assays, respectively. Expression of MMP-2 and activity of MMP-9 were observed by immunoblotting and gelatin zymography, respectively. Results: ATX up to 150 µM was not toxic to A172 cells at 48 h post-treatment. In contrast, ATX at 50 and 100 µM significantly decreased migration and invasion of A172 cells at 24 and 48 h post-treatment. Metastatic-reducing effect of ATX is associated with the reduction of MMP-2 and MMP-9 expressions in a dose-dependent manner. Conclusion: This finding indicated that ATX has anti-migration and invasion effects against human glioblastoma cells and might be applicable for the protection against metastasis of glioblastoma. UR - https://journal.waocp.org/article_89172.html L1 - https://journal.waocp.org/article_89172_568fd2d0847135cc8c4320943bcdb66a.pdf ER -