TY - JOUR ID - 89490 TI - Underexpression of miR-126-3p in Patients with Cholangiocarcinoma JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Poleto Spinola, Lucas AU - F Vieira, Gabriel AU - Fernandes Ferreira, Rafael AU - Calastri, Maria C J AU - D Tenani, Graciele AU - Aguiar, Franciana L AU - Santana Ferreira Boin, Ilka F AU - B E da Costa, Larissa AU - Chaim Correia, Maria Fernanda AU - Zanovelo, Eliane M AU - B de Souza, Daniele C AU - Martins Alves da Silva, Rita C AU - Ferreira da Silva, Renato AU - Coelho Abrantes, Ana Margarida AU - R R Botelho, Maria Filomena AU - L R Tralhão, Jose Guilherme AU - R S Souza, Doroteia AD - Department of Molecular Biology, São José do Rio Preto Medical School (FAMERP), São Paulo, Brazil. AD - School of Medical Sciences of the State University of Campinas (UNICAMP), Campinas, Brazil. AD - Base Hospital (HB), São José do Rio Preto, São Paulo, Brazil. AD - Medical School of the University of Coimbra, Coimbra, Portugal. Y1 - 2021 PY - 2021 VL - 22 IS - 2 SP - 573 EP - 579 KW - cholangiocarcinoma KW - miR-126-3p KW - Subexpression KW - Life habits KW - Comorbidities DO - 10.31557/APJCP.2021.22.2.573 N2 - Objectives: To evaluate the expression of miR-126-3p and its potential as a biomarker for cholangiocarcinoma (CCA) and to better understand the prognosis, comorbidities, and lifestyle habits associated with the disease. Methods: Fifty-nine individuals were distributed into either the study group (38 CCA patients) or the control group (21 individuals without liver diseases). Total RNA was extracted, cDNA synthesis was performed, and miR-126-3p expression was assessed using real-time PCR. For statistical analysis, alpha error was set at 5%. Results: MiR-126-3p was found to be underexpressed in the study group relative to the controls (0.42; P=0.001). Additionally, marked underexpression was found in the study group in when associated with smoking (0.28; P=0.0001), alcoholism (0.19; P=0.0001), hypertension (0.29; P=000.1), and diabetes (0.12; P=0.0003) relative to the controls. No association was found between miR-126-3p expression and tumor subtypes (iCCA=0.42; pCCA=0.45; dCCA=0.72; P=0.9155). A total of 67% of dCCA patients were event-free at 16 months of follow up, while both pCCA and iCCA exhibited event-free survival rates of 25%, though there was no significant difference between these subgroups (P=0.273). Conclusion: The underexpression of mir-126-3p is associated with cholangiocarcinoma and can be potentiated by alcoholism, hypertension, diabetes, and smoking, the latter of which is an independent risk factor for this cancer. Furthermore, dCCA patients exhibit higher survival rates relative to patients with pCCA and iCCA.   UR - https://journal.waocp.org/article_89490.html L1 - https://journal.waocp.org/article_89490_e55a157bb456bf5f284550c361af3dda.pdf ER -