TY - JOUR ID - 89699 TI - Anti-Glioma Effect of Pseudosynanceia melanostigma Venom on Isolated Mitochondria from Glioblastoma Cells JO - Asian Pacific Journal of Cancer Prevention JA - APJCP LA - en SN - 1513-7368 AU - Ramezani, Maral AU - Samiei, Fatemeh AU - Pourahmad, Jalal AD - Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Y1 - 2021 PY - 2021 VL - 22 IS - 7 SP - 2295 EP - 2302 KW - Stone fish venom KW - mitochondria KW - Glioblastoma KW - Anticancer KW - Pseudosynanceia Melanostigma DO - 10.31557/APJCP.2021.22.7.2295 N2 - Background: Glioblastoma is the most common primary malignant tumor of the central nervous system thatoccurs in the spinal cord or brain. Pseudosynanceia melanostigma is a venomous stonefish in the Persian Gulf, whichour knowledge about is little. This study’s goal is to investigate the toxicity of stonefish crude venom on mitochondriaisolated from U87 cells. Methods: In the first stage, we extracted venom stonefish and then isolated mitochondria haveexposed to different concentrations of venom. Finally, mitochondrial toxicity parameters (Succinate dehydrogenase(SDH) activity, Reactive oxygen species (ROS), cytochrome c release, Mitochondrial Membrane Potential (MMP),and mitochondrial swelling) have evaluated. Results: To determine mitochondrial parameters, we used 115, 230, and460 μg/ml concentrations. The results of our study show that the venom of stonefish selectively increases upstreamparameters of apoptosis such as mitochondrial swelling, cytochrome c release, MMP collapse and ROS. Conclusion:This study suggests that Pseudosynanceia melanostigma crude venom has selectively caused toxicity by increasingactive mitochondrial oxygen radicals. This venom could potentially be a candidate for the treatment of glioblastoma. UR - https://journal.waocp.org/article_89699.html L1 - https://journal.waocp.org/article_89699_0d202163c5c49c14358cdd210a6768f6.pdf ER -